Isoprenaline Induced Hepatic Alterations and Modulation by Hydroalcoholic Extract of Juglans regia hull in Wistar Rats

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  • Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Science and Animal Husbandry, R S Pura – 181102, Jammu, Jammu and Kashmir ,IN
  • Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Science and Animal Husbandry, R S Pura – 181102, Jammu, Jammu and Kashmir ,IN
  • Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Science and Animal Husbandry, R S Pura – 181102, Jammu, Jammu and Kashmir ,IN
  • Division of Veterinary Pathology, Faculty of Veterinary Science and Animal Husbandry, R S Pura – 181102, Jammu, Jammu and Kashmir ,IN
  • Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary and Animal Sciences, Institute of Agricultural Sciences, Rajiv Gandhi South Campus, Banaras Hindu University, Mirzapur – 231001, Uttar Pradesh ,IN
  • Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Science and Animal Husbandry, R S Pura – 181102, Jammu, Jammu and Kashmir ,IN



Hepatic Tissue, Isoprenaline, Juglans regia, Oxidative Damage


The present study aimed at determining isoprenaline (ISO) induced hepatic alterations and effect of hydroalcoholic extract of Juglans regia hull supplementation in modulating those alterations in Wistar rats. Various biochemical, histopathological and antioxidant biomarkers in hepatic tissue viz. transferases (aspartate and alanine aminotransferases), Total Plasma Proteins (TPP), Albumins (ALB), Total Antioxidant Status (TAS), Total Thiols (TTH), Catalase (CAT), Superoxide Dismutase (SOD), Acetylcholinesterase (AChE), Arylesterase (AE), Glutathione Peroxidase (GPX) and Glutathione Reductase (GR), and cellular damage indicators viz. Malondialdehyde (MDA) and Advanced Oxidation Protein Product (AOPP) were assessed. Intra-peritoneal administration of ISO produced significant increase in aspartate aminotransferase, AChE, MDA and AOPP levels and reduced TPP, ALB, TTH, TAS, AE and GPX, activities in hepatic tissue of rats. Histopathologically, the liver sections of ISO treated rats revealed congestion, vacuolar degeneration and necrosis of liver parenchyma, bile duct hyperplasia and edema along with cellular infiltration in portal triad areas. Pretreatment with hull extract restored TAS, AOPP, AChE, CAT, AE activities and significantly reduced severity of histomorphological changes in hepatic tissue of ISO treated rats. The protective effect may be attributed to phytochemical constituents present in hull. Overall, our results show that hydroalcoholic extract of J. regia hull confers partial protection to hepatic tissue against ISO induced oxidative changes in Wistar rats.


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Sharma, P., Kumar Verma, P., Raina, R., Sood, S., Prawez, S., & Manhas, L. (2020). Isoprenaline Induced Hepatic Alterations and Modulation by Hydroalcoholic Extract of <i>Juglans regia</i> hull in Wistar Rats. Toxicology International, 27(1&amp;2), 58–69.



Research Articles
Received 2020-05-12
Accepted 2020-06-24
Published 2020-10-01



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