Postnatal Exposure to Sodium Arsenite (NaAsO2) Induces Long Lasting Effects in Rat Testes

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  • Department of Anatomy, All India Institute of Medical Sciences, Ansari Nagar, New Delhi- 110029 ,IN
  • Department of Anatomy, All India Institute of Medical Sciences, Ansari Nagar, New Delhi- 110029 ,IN
  • Department of Anatomy, Srinivas Institute of Medical Science and Research Centre, Mukka, Manglore-574146 ,IN
  • Department of Anatomy, All India Institute of Medical Sciences, Ansari Nagar, New Delhi- 110029 ,IN


Arsenic, Postnatal period, Seminiferous tubules, sodium arsenite, spermatozoa, testis


Objective: The present study was undertaken to investigate the effects of early postnatal exposure to sodium arsenite (NaAsO2) on rat testis. Materials and Methods: Wistar rat pups were administered aqueous solution of NaAsO2, 1.5 mg/kg body weight (bw) (experimental) and distilled water (control), respectively, by intraperitoneal route (i.p.) from postnatal day (PND) 1 to 14. Testes were collected after 1, 7 and 36 days (at PND 15, 21 and 50) after the treatment period (PND1-14) from the animals and immersion fixed in Bouin's fluid followed by paraffin embedding. Seven micrometer thick serial sections were cut and stained with hematoxylin and eosin for light microscopic observations. At PND 50, morphological features of sperms and their counting was carried out besides processing the perfusion-fixed testes for electron microscopy (EM). Results and Conclusions: The observations revealed an altered morphology of the seminiferous tubules (ST) along with degeneration and dissociation of spermatogenic cells in the experimental animals at PND 15, 21 and 50. Also, increased number of sperms with abnormal morphology and decreased sperm count was noted in the experimental animals. These features together with electron microscopic observations of abnormal mitochondria and apoptotic nuclei of spermatogonia and spermatocytes could be indicative of long-lasting adverse effects on the rat testis induced by exposure to As during early postnatal period.


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Kaushal, P., Dhar, P., Shivaprasad, S. M., & Mehra, R. D. (2018). Postnatal Exposure to Sodium Arsenite (NaAsO<sub>2</sub>) Induces Long Lasting Effects in Rat Testes. Toxicology International, 19(2), 215–222. Retrieved from



Original Research
Received 2018-05-25
Accepted 2018-05-25
Published 2018-05-25



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