Arsenic Hyper-tolerance in Four Microbacterium Species Isolated from Soil Contaminated with Textile Effluent

Jump To References Section


  • Department of Life Sciences, The IIS University, Jaipur-302020 ,IN
  • Department of Life Sciences, The IIS University, Jaipur-302020 ,IN
  • Department of Life Sciences, The IIS University, Jaipur-302020 ,IN
  • Department of Life Sciences, The IIS University, Jaipur-302020 ,IN
  • Department of Life Sciences, The IIS University, Jaipur-302020 ,IN
  • Division of Pharmacology and Toxicology, Defence Research & Development Establishment, Gwalior - 474 002 ,IN
  • Division of Pharmacology and Toxicology, Defence Research & Development Establishment, Gwalior - 474 002 ,IN


Arsenic, bacteria, hyper-tolerance, metal-resistant


Arsenic-contaminated areas of Sanganer, Jaipur, Rajasthan, India were surveyed for the presence of metal resistant bacteria contaminated with textile effluent. Samples were collected from soil receiving regular effluent from the textile industries located at Sanganer area. The properties like pH, electrical conductivity, organic carbon, organic matter, exchangeable calcium, water holding capacity and metals like arsenic, iron, magnesium, lead and zinc were estimated in the contaminated soil. In total, nine bacterial strains were isolated which exhibited minimum inhibitory concentration (MIC) of arsenic ranging between 23.09 and 69.2mM. Four out of nine arsenic contaminated soil samples exhibited the presence of arsenite hyper-tolerant bacteria. Four high arsenite tolerant bacteria were characterized by 16S rDNA gene sequencing which revealed their similarity to Microbacterium paraoxydans strain 3109, Microbacterium paraoxydans strain CF36, Microbacterium sp. CQ0110Y, Microbacterium sp. GE1017. The above results were confirmed as per Bergey's Manual of Determinative Bacteriology. All the four Microbacterium strains were found to be resistant to 100μg/ml concentration of cobalt, nickel, zinc, chromium selenium and stannous and also exhibited variable sensitivity to mercury, cadmium, lead and antimony. These results indicate that the arsenic polluted soil harbors arsenite hyper-tolerant bacteria like Microbacterium which might play a role in bioremediation of the soil.


Download data is not yet available.



How to Cite

Kaushik, P., Rawat, N., Mathur, M., Raghuvanshi, P., Bhatnagar, P., Swarnkar, H., & Flora, S. (2018). Arsenic Hyper-tolerance in Four <i>Microbacterium</i> Species Isolated from Soil Contaminated with Textile Effluent. Toxicology International, 19(2), 188–194. Retrieved from



Original Research
Received 2018-05-24
Accepted 2018-05-24
Published 2018-05-25



Wong MF, Chua H, Lo W, Leung CK, Yu PH. Removal and recovery of copper (II) ions by bacterial biosorption. Appl Biochem Biotechnol 2001;91-93:447-57.

Lei W, Chua H, Lo WH, Yu PH, Zhao YG, Wong PK. A novel magnetite--immobilized cell process for heavy metal removal from industrial effluent. Appl Biochem Biotechnol 2000;8486:1113-26.

Mandal BK, Suzuki KT. Arsenic round the world: a review. Talanta 2002;58:201-35.

Flora SJ. Arsenic-induced oxidative stress and its reversibility. Free Radic Biol Med 2011;51:257-81.

Suresh K, Prabagaran SR, Sengupta S, Shivaji S. Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India. Int J Syst Evol Microbiol 2004;54:1369-75.

Maiti SK. Analysis of Chemical Parameters of soil. In book: Handbook of Methods in Environmental Studies: Air, Noise, Soil and Overburden analysis. Vol. 2. Chapter 11. Jaipur, India: ABD Publishers; 2003. p. 162-209.

Courvalin P, Goldstein F, Philippon A, Sirot J. Fiches techniques d'étude pratique des antibiotiques. In L'antibiogramme, 1st ed. Paris: MPC-Videom; 1985. p. 237-44.

Sacchi CT, Whitney AM, Mayer LW, Morey R, Steigerwalt A, Boras A, et al. Sequencing of 16S rRNA gene: a rapid tool for identification of Bacillus anthracis. Emerg Infect Dis 2002;8:1117-23.

Maniatis T, Sambrook J, Fritsch EF. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor: Cold Spring Harbor Laboratory; 1989.

Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406-25.

Felsenstein J. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 1985;39:783-91.

Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111-20.

Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007;24:1596-9.

Salam MA, Hossain MS, Ali ME, Asad MA, Ali MH. Isolation and characterization of arsenic resistant bacteria from different environment in South--West region of Bangladesh. Res J Environ Sci 2009;3:110-5.

Dave SR, Gupta KH, Tipre DR. Diversity of arsenite--resistant cocci isolated from Hutti Gold Mine and bioreactor sample. Curr Sci 2010;98:1229-33.

Chen S, Shao Z. Isolation and diversity analysis of arseniteresistant bacteria in communities enriched from deep--sea sediments of the Southwest Indian Ocean Ridge. Extremophiles 2009;13:39-48.

Patel PC, Goulhen F, Boothman C, Gault AG, Charnock JM, Kalia K, et al. Arsenate detoxification in a Pseudomonad hypertolerant to arsenic. Arch Microbiol 2007;187:171-83.

Anderson CR, Cook GM. Isolation and characterization of arsenate--reducing bacteria from arsenic-contaminated sites in New Zealand. Curr Microbiol 2004;48:341-7.

Cai J, Salmon K, DuBow MS. A chromosomal ars operon homologue of Pseudomonas aeruginosa confers increased resistance to arsenic and antimony in Escherichia coli. Microbiology 1998;144:2705-13.

Joshi DN, Patel JS, Flora SJ, Kalia K. Arsenic accumulation by Pseudomonas stutzeri and its response to some thiol chelators. Environ Health Prev Med 2008;13:257-63.

Most read articles by the same author(s)