Ameliorative Potential of Psidium guajava on Hemato-biochemical Alterations in Arsenic-exposed Wistar Rats

Jump To References Section


  • Departments of Veterinary BiocheCollege of Veterinary Science and Animal Husbandry, Anjora, Durg, Chhattisgarh ,IN
  • Departments of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Anjora, Durg, Chhattisgarh ,IN
  • Departments of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Anjora, Durg, Chhattisgarh ,IN


Hemato-biochemical, rats, sodium arsenite, toxicity Original Article products


The present study attempts to investigate the effects of Psidium guajava (P. guajava) when administered in combination with sodium arsenite @ 20 ppm in drinking water with the aim of achieving normalization of altered biochemical, hematological parameters suggestive of hepatic damage and depletion of inorganic arsenic following chronic arsenic exposure. Thirty adult Wistar rats were given 20 ppm arsenic for eight weeks along with hydro alcoholic leaf extract of P. guajava at a dose of 100 mg/kg body weight wt. (orally) (once daily for eight weeks). Arsenic exposure led to significant depletion of hemoglobin, red blood cells (RBC) and packed cell volume (PCV) but elevated leucocyte count (TLC). There was a significant increase (P<0.01/P<0.05) in serum aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphotase (ALP), acid phosphotase (ACP) and blood glucose whereas decrease in total protein level in arsenic-exposed untreated animals. The changes were accompanied by a significant elevation in blood and soft-tissue arsenic concentration. Co-administration of P. guajava was most effective not only in reducing arsenic-induced hematological and biochemical alterations but also in depleting arsenic from blood and soft tissues following arsenic exposure. We thus recommend combined leaf extract of P. guajava for achieving optimum effects of chelation therapy.


Download data is not yet available.



How to Cite

Tandan, N., Roy, M., & Roy, S. (2018). Ameliorative Potential of <i>Psidium guajava<i> on Hemato-biochemical Alterations in Arsenic-exposed Wistar Rats. Toxicology International, 19(2), 121–124. Retrieved from



Original Research



Cebrian ME, Albores A, Aguilae M, Blakely E. Chronic arsenic poisoining in the North Mexico. Hum Toxicol 1983;2:121-33.

Flora SJ, Chouhan S, Kannan GM, Mittal M, Swarnkar H. Combined administration of taurine and monoisoamyl DMSA protects arsenic induced oxidative injury in rats. Oxid Med Cell Longev 2008;1:39-45.

Snedeor GW, Cochran WG. Statistical methods. 8th ed. New Delhi: Affiliated East-West Press; 1994.

Ferzand R, Gadahi JA, Saleha S, Ali Q. Histological and haematological disturbances caused by arsenic toxicity in mice model. Pak J Biol Sci 2008;11:1405-13.

Padmaja B, Madhuri D, Kumar A, Anjaneyulu Y. Ameliorative efficacy of Emblica officinalis in arsenic induced toxicity in broilers: A haemato-biochemical study. Indian J Vet Pathol 2009;33:124-9.

Mittal M, Flora SJ. Vitamin E supplementation protects oxidative stress during arsenic and fluoride antagonism in male mice. Drug Chem Toxicol 2007;30:263-81.

Kaneko JJ. Clinical biochemistry of domestic animals. 3rd ed. New York: Academic Press; 1980.

Tseng CH, Tseng CP, Chiou HY, Hsueh YM, Chong CK, Chen CJ. Epidemiologic evidence of diabetogenic effect of arsenic. Toxicol Lett 2002;133:69-76.

Biswas S, Talukdar G, Sharma A. Protection against cytotoxic effects of arsenic by dietry supplementation with crude extract of Emblica officinalis fruit. Phytother Res 1999;13:513-6.

Roy M. Studies on biochemical effects of some environmental toxins and mitigation of adverse effects. Thesis submitted to Pt. Ravishankar Shukla University; 2006.

Santra A, Maiti A, Das S, Lahiri S, Charkaborty SK, Mazumder DN. Hepatic damage caused by chronic arsenic toxicity in experimental animals. J Toxicol Clin Toxicol 2000;38:395-405.

Banerjee P, Bhattacharya SS, Bhattacharjee N, Pathak S, Boujedaini N, Belon P, et al. Ascorbic acid combats arsenicinduced oxidative stress in mice liver. Ecotoxicol Environ Saf 2009;72:639-49.

Verma, RJ Vasu A, Saiyed AA. Arsenic toxicity in mice and its possible amelioration. J Environ Sci (China) 2004;16:447-53.

Golub MS, Keen CL, Commisso JF, Saocks CB, Hathaway TR. Arsenic tissue concentration of immature mice one hour after oral exposure to gold mine tailing. Environ Geochem Health 1999;21:199-209.

Kamaluddin M, Misbahuddin M. Zinc supplement on tissue arsenic concentration in rats. Bangladesh Med Res Counc Bull 2006;32:87-91.