Protective Effect of Psidium guajava in Arsenic-induced Oxidative Stress and Cytological Damage in Rats



Histopahtology, oxidative stress, Psidium guajava, rats, sodium arsenite


This study was undertaken to evaluate the protective effect of aqueous extract of Psidium guajava leaves against sodium arsenite-induced toxicity in experimental rats. Animals were divided into four groups. Control group received arsenic free distilled water and three treatment groups (II, III, and IV) exposed to the arsenic (NaAsO2) (20 mg/kg b.wt) through drinking water. Group III and IV were administered a daily oral dose of P. guajava leaf extract 50 and 100 mg/kg b.wt. (AEPG50 and AEPG100) for the period of 6 weeks. Blood samples and organs were collected at the end of the experiment. Arsenic exposure resulted in significant rise in lipid peroxidation (LPO) levels in erythrocyte, liver, kidney, and brain. In addition toxin decreased (P100 (aqueous extract of P. guajava) @100 mg/kg body weight) significantly restored activities of oxidative stress markers like LPO levels, GSH levels, SOD, and CAT activities but having the limited protective activity of the herbal extract was observed on tissues architecture. It is therefore concluded that prophylactic co-administration of AEPG could provide specific protection from oxidative injury and to some extent on tissue damage.


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Ranft U, Miskovic P, Pesch B, Jakubis M, Nieuwenhuijsen MJ.EXPASCAN study group Association between arsenic exposure from a coal burning power plant and urinary arsenic concentrations in prievidza district. Slovakia. Environ Health Perspect 2003;111:864-9.

Roy M, Pandey PK, Roy S, Chauhan HV. Arsenic induced haematobiochemical and histopathological alterations and its level in certain biological samples. Toxicol Int 2008;25:57-62.

Sarkar M, Chaudhuri GR, Chattopadhyay A, Biswas NM. Effect of sodium arsenite on spermatogenesis, plasma gonadotrophins and testosterone in rats. Asian J Androl 2003;5:27-31.

Ramos O, Carrizales L, Yáñez L, Mejí­a J, Batres L, Ortí­z D, et al. Arsenic increased lipid peroxidation in rat tissues by a mechanism independent of glutathione levels. Environ Health Perspect 1995;103:85-8.

Droge W. Free radicals in the physiological control of cell function. Physiol Rev 2002;82:47-95.

Chen CJ, Hsueh YM, Lai MS, Shyu MP, Chen SY, Wu MM, et al.Increased prevalence of hypertension and long-term arsenic exposure. Hypertension 1995;25:53-60.

Waalkes MP. Target sites of carcinogenic metals. Metal Toxicology. In: Goyer RA, Klaassen CD, Waalkes MP, editors. New York: Academic Press; 1995. p. 54-6.

Kapoor R, Srivastava S, Kakkar P. Bacopa monnieri modulates antioxidant responses in brain and kidney of diabetic rats.Environ Toxicol Pharmacol 2009;27:62-9.

Placer ZA, Cushman LL, Johnson BC. Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical system.Anal Biochem 1966;16:359-64.

Utley HG, Bernheim F, Hochsein P. Effect of sulphydryl reagents on peroxidation of microsomes. Arch Biochem Biophys 1967;118:29-32.

Van Kampen EJ, Ziglstra WG. Colorimetric determination of hemoglobin. Clin Chem Acta 1961;6:53-88.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75.

Marklund S, Marklund G. Involvement of superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974;47:469-74.

Menami M, Yoshikawa H. A simplified assay method of superoxide dismutase activity of clinical use. Clin Chem Acta 1979;92:337-42.

Cohen G, Dembiec D, Marcus J. Measurement of catalase activity in tissue extract. Anal Biochem 1970;34:30-8.

Hershey JW, Oostdyk TS, Keliher PN. Detection of arsenic and selenium in environmental and agricultural samples by hydride generation atomic absorption spectrophotometry. J Assoc Off Anal Chem 1988;71:1090-3.

Snedecor GW, Cochran WG. Statistical methods. 8th ed. New Delhi: Affiliated East-West Press; 1994. p. 68.

Manna P, Sinha M, Sil PC. Arsenic-induced oxidative myocardial injury: Protective role of arjunolic acid. Arch Toxicol 2008;82:137-49.

Flora SJ, Chouhan S, Kannan GM, Mittal M, Swarnkar H. Combined administration of taurine and monoisoamyl DMSA protects arsenic induced oxidative injury in rats. Oxid Med Cell Longev 2008;1:39-45.

Chaudhuri AN, Basu S, Chattopadhyay S, Das Gupta S. Effect of high arsenic content in drinking water on rat brain. Indian J Biochem Biophys 1999;36:51-4.

Chouchane S, Snow ET. In vitro effect of arsenical compounds on glutathione-related enzymes. Chem Res Toxicol 2001;14;517-22.

Maiti S, Chatterjee AK. Effects on levels of gluathione and some related enzymes in tissues after an acute arsenic exposure ad their relationship to dietary protein deficiency. Arch Toxicol 2001;75:531-7.

Maiti S, Chatterjee AK. Differential response of cellular antioxidant mechanism of liver and kidney to arsenic exposure and its relation to dietary protein deficiency. Environ Toxicol Pharmacol 2000;8:227-35.

McCord JM, Keele BB, Fridovich I. An enzyme based theory of obligate anaerobis: The physiological functions of superoxide dismutase. Proc Natl Acad Sci U S A 1976;68:1024-31.

Gutteridge JM. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 1995;41:1819-28.

Nandi D, Patra RC, Ranjan R, Swarup D. Role of co-administration of antioxidants in prevention of oxidative injury following sub-chronic exposure to arsenic in rats. Veterinarski Arhiv 2008;78:113-21.

Flora SJ, Pant SC, Malhotra PR, Kankan GM. Biochemical and histopathological changes in arsenic intoxicated rats exposed to ethanol. Alcohol 1999;14:563-8.

Ferzand R, Gadahi JA, Saleha S, Ali Q. Histological and haematological disturbances caused by arsenic toxicity in mice model. Pak J Biol Sci 2008;11:1405-13.

Santra A, Chowdhury S, Ghatak S, Biswas A, Krishna G. Arsenic induces apoptosis in mouse liver is mitochondria dependent and disabrogated by N-acetyl cysteine. Gastroenterology 2007;220:146-55.

Schulz JB, Lindenau, J, Seyfried J, Dichgans J. Glutathione, oxidative stress and neurodegeneration. Eur J Biochem 2000;267:4904-11.

Gutierrez RM, Mitchell S, Solis RV. Psidium guajava: A review of its traditional uses, phytochemistry and pharmacology.J Ethnopharmacol 2008;117:1-27.



How to Cite

Tandon, N., Roy, M., Roy, S., & Gupta, N. (2018). Protective Effect of Psidium guajava in Arsenic-induced Oxidative Stress and Cytological Damage in Rats. Toxicology International, 19(3), 245–249. Retrieved from



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