In Vitro Studies on Antioxidant Potential of Apple (Malus domestica) Fructus Extract Nanoparticle

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Authors

  • Sri Utami
     Faculty of Medicine, YARSI University, Central Jakarta - 10510 ,ID
  • Ndaru Andri Damayanti
     Faculty of Medicine, YARSI University, Central Jakarta - 10510 ,ID
  • Nunung Ainur Rahmah
     Faculty of Medicine, YARSI University, Central Jakarta - 10510 ,ID
  • Said Nafik
     Directorate General of Intellectual Property, Ministry of Law and Human Rights, Republic of Indonesia, South Jakarta - 12940 ,ID
  • Betharie Cendera Arrahmani
     Pharmaceutical Bioprocessing Engineering, Technical University Munich, Freising ,DE
  • Anis Syabani Muthmainnah
     Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung - 40163 ,ID
  • Hanna Sari Widya Kusuma
     Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung - 40163 ,ID
  • Wahyu Widowati
     Faculty of Medicine, Maranatha Christian University, Bandung - 40164 ,ID

DOI:

https://doi.org/10.18311/jnr/2023/32238

Keywords:

Antioxidant, DPPH, FRAP, H2O2, Malus domestica, Nanotechnology

Abstract

Oxidative stress results from an imbalance of free radicals and antioxidants in the body. Antioxidants are needed to prevent oxidative stress. A diet rich in fruits and vegetables, which are high in antioxidants, should help avoid oxidative stress. One source of antioxidants is apples (Malus domestica) from the Rosaceae family because they have some bioactive compounds such as catechin, chlorogenic acid, quercetin, and phloridzin. Recently, many studies have used nanotechnology to formulate plant extracts. Due to their size and distinctive physicochemical properties, nanoparticles in plant extracts have various benefits. Analyzing apple extract nanoparticles’ antioxidant capacity was the goal of this work. The synthesized nanoparticles of apples were made by using chitosan, glacial acetic acid, propylene glycol, ethanol, DMSO, and Na-TPP. A dynamic light scattering particle size analyser was used to measure the zeta potential and particle size. Antioxidant activity was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging, Hydrogen Peroxide (H2O2) scavenging activities, and Ferric Reducing Antioxidant Power (FRAP) assay using colorimetric methods. At a concentration of 100 g/ml, the most DPPH was scavenged (80.35%). Apple extract nanoparticles have strong DPPH scavenging activity with IC50 = 12.16 ± 2.98 µg/ml and H2O2 scavenging activity with IC50 = 81.96 ± 7.23 µg/ml. The highest H2O2 scavenging activity was at 200 µg/ml concentration (84.47%) and the highest FRAP activity was at a concentration of 50 µg/ml (444.29%). The concentration is directly proportional to the antioxidant activity of apple extract nanoparticles. Based on this study, apple extract nanoparticle has strong antioxidant activity.

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Published

2023-11-10

How to Cite

Utami, S., Damayanti, N. A., Rahmah, N. A., Nafik, S., Arrahmani, B. C., Muthmainnah, A. S., Widya Kusuma, H. S., & Widowati, W. (2023). <i>In Vitro</i> Studies on Antioxidant Potential of Apple (<i>Malus domestica</i>) Fructus Extract Nanoparticle. Journal of Natural Remedies, 23(4), 1589–1598. https://doi.org/10.18311/jnr/2023/32238

Issue

Volume 23 Issue 4 October 2023

Section

Short Communication

License

Copyright (c) 2023 Sri Utami, Ndaru Andri Damayanti, Nunung Ainur Rahmah, Said Nafik, Betharie Cendera Arrahmani, Anis Syabani Muthmainnah, Hanna Sari Widya Kusuma, Wahyu Widowati (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Received 2022-12-27
Accepted 2023-09-14
Published 2023-11-10

 

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