Bio-Antioxidants Protect the Buffalo Bone Marrow Derived Mesenchymal Stem Cells against Oxidative Stress Induced During Freeze-Thaw Cycle


  • College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Department of Veterinary Physiology and Biochemistry, Ludhiana, Punjab, 141001, India
  • Guru Angad Dev Veterinary and Animal Sciences University, Department of Veterinary Pharmacology and Toxicology, Ludhiana, Punjab, 141001, India
  • Guru Angad Dev Veterinary and Animal Sciences University, Department of Veterinary Surgery and Radiology, Ludhiana, Punjab, 141001, India


The present investigation was planned to evaluate the effect of curcumin (CUR) and resveratrol (RES) on oxidative stress indicators (LPO, O2- radical, ROS and TPC), antioxidant status (GSH, GPx, SOD, CAT and GST) and cytotoxicity markers (ALP, LDH, GGT and CK-MB) in mesenchymal stem cells (MSCs) derived from buffalo bone marrow during the freeze-thaw cycle of one month. MSCs were isolated and cultured in high glucose DMEM supplemented with 15% FBS. MSCs were found to be positive for alkaline phosphatase (AP) activity and stem cell markers (CD73 and OCT4). Results revealed a significant reduction in (p≤0.05) in oxidative stress parameters and cytotoxicity markers, while, the levels of cellular antioxidants were found to be significantly increased (p≤0.05) in CUR/RES alone and in combination groups as compared to the control group. In conclusion, the addition of bioantioxidants in the cryopreservation medium has improved the post-thaw cell recovery through suppression of oxidative stress-induced during the freeze-thaw cycle.


Antioxidants, Buffalo, Curcumin, Mesenchymal Stem Cells, Oxidative Stress, Resveratrol

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