Anti-inflammatory, Antibacterial and Acaricidal Activities of Various Leaf Extracts of Bitter Apple, <i>Citrullus colocynthis</i> Schrad (Cucurbitaceae)

Authors

  • Department of Veterinary Pharmacology and Toxicology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana–141004, Punjab ORCID logo http://orcid.org/0000-0002-0863-7714
  • Department of Veterinary Pharmacology and Toxicology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana–141004, Punjab
  • Department of Veterinary Parasitology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana–141004, Punjab
  • Department of Veterinary Parasitology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana–141004, Punjab
  • Department of Surgery and Radiology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana–141004, Punjab

DOI:

https://doi.org/10.18311/ti/2021/v28i1/22821

Keywords:

Antibacterial, Anti-inflammatory, <i>Citrullus colocynthis</i>, <i>Rhipicephalus microplus</i>, Wistar Rats

Abstract

The present study involved the investigation of various leaf extracts of Citrullus colocynthis (bitter apple) viz. alcoholic, acetone and chloroform for their in vivo anti-inflammatory and in vitro antibacterial and acaricidal activities. For in vivo evaluation, doses of the extracts were taken as 50 mg/kg and 100mg/kg and for in vitro experiments, concentrations @5% and 10 % were used in the study. The antibacterial activity was studied against gram +ve (Staphylococcus aureus) and gram –ve (Escherichia coli) bacteria and was compared with standard drug (ampicillin). For anti-inflammatory activity; fifty rats were divided into 10 groups of five animals each. Initially the normal paw volumes of all the animals were noted down. All animals were injected in the sub-planter region of the right hind paw with 1% carrageenan (in normal saline) @ 0.1 ml to induce inflammation. The acaricidal activity of the alcoholic leaf extract was investigated by Adult immersion test against engorged adult females of Rhipicephalus microplus (Canestrini, 1888) (Acari: Ixodidae). No dose-dependent acaricidal activity of the extracts was demonstrated as there was no mortality of the ticks and absence of significant inhibition of oviposition by any of the concentrations of the extracts. Also the results revealed no significant antibacterial activity but all the extracts demonstated significant anti-inflammatory activity with maximum activity in alcoholic extract at higher concentration.

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References

Basu SK. Herbal medicines concepts and perspectives. Environ Persp Human Resp. 2002; 3:27–44.

Prajapati ND, Prajapati T, Jajpura S. Advances in medicinal plants (Vol. 1). Asian Med Plants & Health Care Trust. 2005.

Joshi K, Chandra NP, Vandana AK, Kumar A. Antioxidant and antibacterial activities of methanol, ethanol and aqueous extracts of leaf and root of Gentiana kurroo. Ann Phytomed. 2017; 6(2):138–8. https://doi.org/10.21276/ap.2017.6.2.14

Ebadi MS. Herb-drug interactions. Pharmacodynamic Basis of Herbal Medicine. 2nd ed. Boca Raton, Flordia: CRC Press, Taylor and Francis; 2007. p. 37–46. https://doi.org/10.1201/9781420006452.ch4

Nooreen Z, Rai VK, Yadav NP. Phytopharmaceuticals: A new class of drug in India. Ann Phytomed. 2018; 7(1):27–37. https://doi.org/10.21276/ap.2018.7.1.4

Singh H, Dumka VK, Sagar R, Kumar V. Evaluation of antipyretic, anti-inflammatory and analgesic activities of various leaf extracts of Kigelia africana. J Pharmacogn Phytochem. 2017; 6:1024–27.

Roy RK, Thakur M, Dixit VK. Effect of Citrullus colo cynthis on hair growth in albino rats. Pharm Biol. 2007; 45(10):739–44. https://doi.org/10.1080/13880200 701585709

Tannin-Spitz, T, Grossman S, Dovrat S, Gottlieb HE, Bergman M. Growth inhibitory activity of cucurbitacin glycosides isolated from Citrullus colocynthis on human breast cancer cells. Biochem Pharmacol. 2007; 73(1):56–67. https://doi.org/10.1016/j.bcp.2006.09.012. PMid:17049494

Gurudeeban S, Ramanathan, T. Antidiabetic effect of Citrullus colocynthis in alloxon-induced diabetic rats. Inventi Rapid: Ethno pharmacol. 2010; 1:112.

Collin CH, Lyne PM, Grange JM. Microbiological methods. 7th edn., Butterwort - Heinemann Ltd, Britain; 1995. p. 175–190.

Winter CA, Risley EA, Nuss GW. Carrageenininduced edema in hind paw of the rat as an assay for anti inflammatory drugs. Proc Soc Exp Biol. 1962; 111(3):544–7. https://doi.org/10.3181/00379727-111-27849. PMid:14001233

Singh NK, Rath SS. Esterase mediated resistance against synthetic pyrethroids in field populations of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) in Punjab districts of India. Vet Parasitol. 2014; 204(3– 4):330–8. https://doi.org/10.1016/j.vetpar.2014.05.035. PMid:24927858

Marzouk B, Marzouk Z, Décor R, Edziri H, Haloui E, Fenina N, et al. Antibacterial and anticandidal screening of Tunisian Citrullus colocynthis Schrad from Medenine. J Ethnopharmacol. 2009; 125(2):344–9. https://doi.org/10.1016/j.jep.2009.04.025. PMid:19397972

Memon U, Brohi AH, Ahmed SW, Azhar I, Bano H. Antibacterial screening of Citrullus colocynthis. Pak J Pharm Sci. 2003; 16(1):1–6.

Di Rosa ML, Giroud JP, Willoughby DA. Studies of the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine. J Pathol. 1971; 104(1):15–29. https://doi.org/10.1002/ path.1711040103. PMid:4398139

Published

2021-03-24

How to Cite

Sagar, R., Dumka, V. K., Singh, N. K., Jyoti, ., & Mohindroo, J. (2021). Anti-inflammatory, Antibacterial and Acaricidal Activities of Various Leaf Extracts of Bitter Apple, <i>Citrullus colocynthis</i> Schrad (Cucurbitaceae). Toxicology International, 28(1), 1–6. https://doi.org/10.18311/ti/2021/v28i1/22821

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Section

Original Research