Serological Changes Induced by Blend of Sunset Yellow, Metanil Yellow and Tartrazine in Swiss Albino Rat, Rattus Norvegicus

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Metanil yellow, sunset yellow, Swiss rat and blend, tartrazine


Objective: The present study was carried out to evaluate the toxic effect of blend of some food colors on Swiss albino rats. Materials and Methods: A blend (1:1:1) of sunset yellow, metanil yellow and tartrazine showed additive effects on serological parameters which indicate that addition of these dye together in food stuff may give rise to more toxic effects than are produced by each dye individually. Animals were divided into four groups (I, II, III, and IV). First group was treated as control and respective group of animals received 25, 50 and 75 mg/kg body weight blend of food colors by gavaging up to 30 days. Results: The serological study showed a decrease in total protein and albumin and an increase in alkaline phosphatase, SGPT and total bilirubin. The results revealed that oral administration of these blend did not affect the body weight gain. Conclusion: The prolonged consumption of the blend may cause adverse effect on human health.


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How to Cite

Saxena, B., & Sharma, S. (2018). Serological Changes Induced by Blend of Sunset Yellow, Metanil Yellow and Tartrazine in Swiss Albino Rat, Rattus Norvegicus. Toxicology International, 21(1), 65–68. Retrieved from



Original Research
Received 2018-04-24
Accepted 2018-04-24
Published 2018-04-25



Sharma A, Goyal RP, Chakravarty G, Sharma S. Haemotoxic effects of chocolate brown, a commonly used blend of permitted food colour on Swiss albino mice. Asian J Exp Sci 2005;19:93"‘103.

Kind PR, King EJ. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino"‘antipyrine. J Clin Pathol 1954;7:322"‘6.

Doumas BT, Perry BW, Sasse EA, Straumfjord JV Jr. Standardization in bilirubin assays: Evaluation of selected methods and stability of bilirubin solutions. Clin Chem 1973;19:984"‘93.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265"‘75.

Gautam D, Sharma G, Goyal RP. Evaluation of toxic impact of tartrazine on male Swiss albino mice. Pharmacologyonline 2010;1:133"‘40.

Hasan GM. Effects of some synthetic coloring additives on DNA damage and chromosomal aberrations of rats. Arab J Biotech 2010;13:13"‘24.

Chatterjee MN, Shinde R. Diet and nutrition. In: Textbook of Medical Biochemistry. 5th ed. New Delhi: Jaypee Brothers Medical Publishers (P) Ltd.; 2002. p. 683"‘5.

Osman MA, Afifi A, Hussien RM, Kamilia B, Aziz A, Salah SH. Long term biochemical and genotoxicity studies of four synthetic food and drug colourant in mice. Bull Fac Pharm 1995;33:12"‘3.

Sharma S, Goyal RP, Chakravarty G, Sharma A. Orange red, a blend of permitted food colour induced haematological changes in Swiss albino mice, Mus musculus. Bull Pure App Sci 2005; 24:99"‘103.

Sharma A, Goyal RP, Chakravarty G, Sharma S. Toxicological studies on effect of apple green"‘a permitted food colour on Swiss albino mice, Mus musculus. Ind J Environ Sci 2006;10:21"‘4.

Chakravarty G, Goyal RP, Sharma S, Sharma A. Haematolgical and serological studies on Swiss albino mice fed with lead chromate. J Ecot Environ Monit 2007;17:61"‘6.

Sharma G, Gautam D, Goyal RP. Tartrazine induced haematological and serological changes in female Swiss albino mice, Mus musculus. Pharmacologyonline 2009;3:774"‘88.

Abdel Aziz AH, Shouman SA, Attia AS, Saad SF. A study on the reproductive toxicity of erythrosine in male mice. Pharmacol Res 1997;35:457"‘62.

HelalEG, Zahkouk SA, Mekawy HA. Effect of some food colorants (synthetic and natural products) of young albino rats. Egypt J Hosp Med 2000;1:100"‘13.

Singh RL. Effect of protein malnutrition on biochemical parameters of serum and liver of metanil yellow exposed rats. Toxicol Environ Chem 1996;54:107"‘13.

Helal EG. Progressive effects of the interaction of sodium nitrite and sunset yellow on different physiological parameters in albino rats. Egypt J Hosp Med 2001;2:23"‘46.

Mahmoud NH. Toxic effect of the synthetic food dye brilliant blue on liver, kidney and testes function in rats. J Egypt Soc Toxicol 2006;34:77"‘84.

Sharma G, Sharma S, Dixit A, Gautam D and Goyal RP. Effect of kesari powder on haematological and serological parameters in female swiss albino mice. Pharmacologyonline 2010; 2: 425"‘444.

AshourAA, Abdelaziz I. Role of fast green on the blood of rats and the therapeutic action of vitamins C and E. Inter J Intergra Biol 2009;6:6"‘11.

Chakravarty G, Goyal RP, Sharma S, Sharma A. Heamatological changes induced by a common non"‘permitted food colour malachite green (MG) in Swiss albino mice. Ind J Env Sci 2005;9:113"‘7.

Latha BV, Jeevaratanm K. Thirteen"‘week oral toxicity study of carotenoid pigment from Rhodoturula glutinis DFR"‘PDY in rats. Indian J Exp Biol 2012;50:645"‘51.

Himri I, Bellahcen S, Souna F, Belmekki F, Aziz M, Bnouham M, et al. A 90"‘day oral toxicity study of tartrazine, a synthetic food dye, in wistar rats. Int J Pharm Pharm Sci 2011;3:159"‘69.