Evaluation of Analgesic, Antidiarrheal and Hypoglycemic Activities of Wendlandia paniculata (Roxb.) DC Leaves Extract using Mice Model

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  • Department of Chemistry, JUST, Jashore – 7408 ,BD
  • Department of Environmental Science and Technology, JUST, Jashore – 7408 ,BD
  • Department of Pharmacy, SUB, 77 Satmasjid road, Dhanmandi, Dhaka – 1205 ,BD
  • Department of Chemistry, DU, Dhaka – 1000 ,BD




Analgesic, Antidiarrheal, Hypoglycemic, Wendlandia paniculata


The leaves of Wendlandia paniculata (Roxb.) DC is a rare folk medicine among tribal people even though there is no scientific literature available regarding its pharmacological potentials. This study aimed to evaluate the effects of crude methanol extract of W. paniculata (Roxb.) DC. Leaves in mice model. The in vivo glucose-lowering capacity of methanolic crude extract was analyzed utilizing the well-known tail tipping method. The central and peripheral analgesic activity was evaluated using the ‘tail flick' and writhing assay respectively. The anti-diarrheal activity was evaluated using the method of castor oil-induced diarrhea in mice. As a result, oral administration (400 mg/ kg) of the extract resulted significant (P<0.001) delay in response to pain and inhibition of acetic acid-induced writhing response. The results were comparable to the respective standards morphine (2 mg/kg) and diclofenac (50 mg/kg). Likewise, maximum reduction (p<0.05) of blood glucose (32.3%) was observed 180 min after oral intake(400 mg/kg) of the extract compared to the 47% of the standard glibenclamide. Moreover, a reduction of diarrheal feces was observed 45.83% (p<0.001) compared to standard loperamide 70.83%. The in vivo bioassays confirmed that the crude methanol extract of leaves of W. paniculata possesses significant analgesic (central, peripheral) and antidiarrheal activity with mild glucose-lowering activity. Isolation of bioactive compounds is needed to justify the molecule responsible for the activity.


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How to Cite

Mahmudul Hasan, M., Sabbir Hossain, M., Abdullah Taher, M., & Rahman, T. (2021). Evaluation of Analgesic, Antidiarrheal and Hypoglycemic Activities of <i>Wendlandia paniculata</i> (Roxb.) DC Leaves Extract using Mice Model. Toxicology International, 28(2), 155–163. https://doi.org/10.18311/ti/2021/v28i2/26775



Original Research
Received 2021-01-18
Accepted 2021-04-01
Published 2021-05-31



Ghani A. Medicinal plants of Bangladesh: chemical constituents and uses. Asiat Soc Bangladesh. 1998.

Das S, Choudhury MD. Plants used against gastrointestinal disorders and as anti-hemorrhagic by three tribes of North Tripura district, Tripura, India: A Report. Ethnobot leafl. 2010; 2010(4):10.

Behera KK. Ethnomedicinal plants used by the tribals of Similipal Bioreserve, Orissa, India: A pilot study. Ethnobot leafl. 2006; 2006(1):17.

Balick MJ. Transforming ethnobotany for the new millennium. Ann Mo Bot Gard. 1996.p. 58–66. https://doi.org/10.2307/2399968 DOI: https://doi.org/10.2307/2399968

Handa SS. Indian efforts on standardization and quality control of medicinal plants using scientific parameters. Amruth (The Traditional Healthcare Magazine). 1998; 2(10).

Schultes RE, Raffauf RF. The healing forest: medicinal and toxic plants of the Northwest Amazonia. Dioscorides Press; 1990.

Rojas A, Hernandez L, Pereda-Miranda R, Mata R. Screening for antimicrobial activity of crude drug extracts and pure natural products from Mexican medicinal plants. J Ethnopharmacol. 1992; 35(3):275– 83. https://doi.org/10.1016/0378-8741(92)90025-M DOI: https://doi.org/10.1016/0378-8741(92)90025-M

Prance GT. Richard Evans Schultes (12 January 1915- 10 April 2001): A tribute. Econ Bot. 2001; 55(3):347–62. https://doi.org/10.1007/BF02866558 DOI: https://doi.org/10.1007/BF02866558

Cox PA. The ethnobotanical approach to drug discovery: Strengths and limitations. Ethnobotany and the search for new drugs. 1994; 2541.

Farnsworth NR. The role of medicinal plants in drug development. Nat Prod Drug Dev. 1984; 17:30–4.

Rahman MA, Uddin SB, Wilcock CC. Medicinal plants used by Chakma tribe in Hill Tracts districts of Bangladesh. IJTK.2007.

Maiti R, Jana D, Das UK, Ghosh D. Antidiabetic effect of aqueous extract of seed of Tamarindus indica in streptozotocin-induced diabetic rats. J Ethnopharmacol. 2004; 92(1):85–91. https://doi.org/10.1016/j.jep.2004.02.002. PMid:15099853 DOI: https://doi.org/10.1016/j.jep.2004.02.002

Wadkar KA, Magdum CS, Patil SS, Naikwade NS. Antidiabetic potential and Indian medicinal plants. J Herb Med Toxicol. 2008; 2(1):45–50.

Hui H, Tang G, Go VL. Hypoglycemic herbs and their action mechanisms. Chin Med. 2009; 4(1):1–1. https://doi.org/10.1186/1749-8546-4-11. PM id:19523223. PMCid:PMC2704217 DOI: https://doi.org/10.1186/1749-8546-4-11

Masuda R, Ajimi J, Murata T. Pharmacotherapy for neuropathic pain in Japan. J Nippon Med Sch. 2017; 84(6):258–67. https://doi.org/10.1272/jnms.84.258. PM id:29279555 DOI: https://doi.org/10.1272/jnms.84.258

Tamba BI, Leon MM, Petreus T. Common trace elements alleviate pain in an experimental mouse model. J Neurosci Res. 2013; 91(4):554–61. https://doi.org/10.1002/jnr.23191. PMid:23362003 DOI: https://doi.org/10.1002/jnr.23191

Gedin F, Skeppholm M, Burström K, Sparring V, Tessma M, Zethraeus N. Effectiveness, costs and costeffectiveness of chiropractic care and physiotherapy compared with information and advice in the treatment of non-specific chronic low back pain: study protocol for a randomised controlled trial. Trials. 2017; 18(1):1–2. https://doi.org/10.1186/s13063-017-2351-3. PMid: 29273083. PMCid:PMC5741874 DOI: https://doi.org/10.1186/s13063-017-2351-3

Ullah R, Ahmad S, Atiq A, Hussain H, Rehman N, Abd Elsalam NM, Adnan M. Quantification and antibacterial activity of flavonoids in coffee samples. Afr J Tradit Complement Altern Med. 2015; 12(4):84– 6. DOI: https://doi.org/10.4314/ajtcam.v12i4.13

Ayaz M, Junaid M, Ullah F, Sadiq A, Subhan F, Khan MA, et al. Molecularly characterized solvent extracts and saponins from Polygonum hydropiper L. show high anti-angiogenic, anti-tumor, brine shrimp, and fibroblast NIH/3T3 cell line cytotoxicity. Front Pharmacol. 2016; 7:74. https://doi.org/10.3389/fphar.2016.00074 DOI: https://doi.org/10.3389/fphar.2016.00074

Yakubu MT, Salimon SS. Antidiarrhoeal activity of aqueous extract of Mangifera indica L. leaves in female albino rats. J Ethnopharmacol. 2015; 163:135–41. https://doi.org/10.1016/j.jep.2014.12.060. PMid:25575467 DOI: https://doi.org/10.1016/j.jep.2014.12.060

Schiller LR, Pardi DS, Sellin JH. Chronic diarrhea: Diagnosis and management. Clin Gastroenterol Hepatol. 2017; 15(2):182–93. https://doi.org/10.1016/j.cgh.2016.07.028. PMid:27496381 DOI: https://doi.org/10.1016/j.cgh.2016.07.028

Whyte LA, Al-Araji RA, McLoughlin LM. Guidelines for the management of acute gastroenteritis in children in Europe. Arch Dis Child Educ Pract. 2015; 100(6):308–12. https://doi.org/10.1136/archdis child-2014-307253. PMid:25939578 DOI: https://doi.org/10.1136/archdischild-2014-307253

Teke GN, Kuiate JR, Ngouateu OB, Gatsing D. Antidiarrhoeal and antimicrobial activities of Emilia coccinea (Sims) G. Don extracts. J Ethnopharmacol. 2007; 112(2):278–83. https://doi.org/10.1016/j.jep.2007.03.007. PMid:17433589 DOI: https://doi.org/10.1016/j.jep.2007.03.007

Kitaoka M, Miyata ST, Unterweger D, Pukatzki S. Antibiotic resistance mechanisms of Vibrio cholerae. J Med Microbiol. 2011; 60(4):397–407. https://doi.org/10.1099/jmm.0.023051-0. PMid:21252269 DOI: https://doi.org/10.1099/jmm.0.023051-0

Pérez GS. A study of the antidiarrheal properties of Loeselia mexicana on mice and rats. Phytomedicine. 2005; 12(9):670–4. https://doi.org/10.1016/j.phymed. 2004.01.010. PMid:16194055 DOI: https://doi.org/10.1016/j.phymed.2004.01.010

Kumar R, Sharma RJ, Bairwa K, Roy RK, Kumar A. Pharmacological review on natural antidiarrhoel agents. Der Pharma Chem. 2010; 2(2):66–93.

Wang GX, Han J, Zhao LW, Jiang DX, Liu YT, Liu XL. Anthelmintic activity of steroidal saponins from Paris polyphylla. Phytomedicine. 2010; 17(14):1102–5. https://doi.org/10.1016/j.phymed.2010.04.012. PMid:20576414 DOI: https://doi.org/10.1016/j.phymed.2010.04.012

Dubreuil JD. Antibacterial and antidiarrheal activities of plant products against enterotoxinogenic Escherichia coli. Toxins. 2013; 5(11):2009–41. https://doi.org/10.3390/toxins5112009. PMid:24212181. PMC id:PMC3847712 DOI: https://doi.org/10.3390/toxins5112009

Miah MM, Das P, Ibrahim Y, Shajib MS, Rashid MA. In vitro antioxidant, antimicrobial, membrane stabilization and thrombolytic activities of Dioscorea hispida Dennst. Eur J Integr Med. 2018; 19:121–7. https://doi.org/10.1016/j.eujim.2018.02.002 DOI: https://doi.org/10.1016/j.eujim.2018.02.002

Kayser MS, Bashar M.B, Ahmed T, Al Aman DA. In vivo Anti-diarrheal and CNS Depressant Activities of Hemigraphis hirta (Vahl) T. Anders. Bangladesh J Pharmacol. 2019; 22(2):176-80. https://doi.org/10.3329/bpj.v22i2.42301 DOI: https://doi.org/10.3329/bpj.v22i2.42301

Dürschlag M, Würbel H, Stauffacher M, Von Holst DI. Repeated blood collection in the laboratory mouse by tail incision-modification of an old technique. Physiol Behav. 1996; 60(6):1565–8. https://doi.org/10.1016/S0031-9384(96)00307-1 DOI: https://doi.org/10.1016/S0031-9384(96)00307-1

Sharmin T, Rahman MS, Mohammadi H. Investigation of biological activities of the flowers of Lagerstroemia speciosa, the Jarul flower of Bangladesh. BMC Complement Altern Med. 2018; 18(1). https://doi.org/10.1186/s12906-018-2286-6. PMid:30081877. PM Cid:PMC6080514 DOI: https://doi.org/10.1186/s12906-018-2286-6

Owoyele VB, Oloriegbe YY, Balogun EA, Soladoye AO. Analgesic and anti-inflammatory properties of Nelsonia canescens leaf extract. J Ethnopharmacol. 2005; 99(1):153–6. https://doi.org/10.1016/j.jep.2005. 02.003. PMid:15848036 DOI: https://doi.org/10.1016/j.jep.2005.02.003

Saha D, Paul S. Study of antidiarrhoeal activity of two Bangladeshi medicinal plants in castor-oil induced diarrhoea. Res J Pharm Technol. 2012; 5(6):800–4.

Michael UA, David BU, Theophine CO, Philip FU, Ogochukwu AM, Benson VA. Antidiabetic effect of combined aqueous leaf extract of Vernonia amygdalina and metformin in rats. J Basic Clin Pharm. 2010; 1(3):197.

Zheng T, Shu G, Yang Z, Mo S, Zhao Y, Mei Z. Antidiabetic effect of total saponins from Entada phaseoloides (L.) Merr. in type 2 diabetic rats. J Ethnopharmacol. 2012; 139(3):814–21. https://doi.org/10.1016/j.jep.2011.12.025. PMid:22212505 DOI: https://doi.org/10.1016/j.jep.2011.12.025

Brahmachari G. Bio-flavonoids with promising antidiabetic potentials: A critical survey. Research signpost. 2011; 661(2):187–212.

Hu X, Wang S, Xu J, Wang DB, Chen Y, Yang GZ. Triterpenoid saponins from Stauntonia chinensis ameliorate insulin resistance via the AMP-activated protein kinase and IR/IRS-1/PI3K/Akt pathways in insulin-resistant HepG2 cells. Int J Mol Sci. 2014; 15(6):10446–58. https://doi.org/10.3390/ijms 150610446. PMid:24918297. PMCid:PMC4100161 DOI: https://doi.org/10.3390/ijms150610446

Adisakwattana S, Roengsamran S, Hsu WH, Yibchok-anun S. Mechanisms of antihyperglycemic effect of p-methoxycinnamic acid in normal and streptozotocin-induced diabetic rats. Life Sci. 2005; 78(4):406–12. https://doi.org/10.1016/j.lfs.2005.04.073. PMid:16139846 DOI: https://doi.org/10.1016/j.lfs.2005.04.073

Sabina E, Chandel S, Rasool MK. Evaluation of analgesic, antipyretic and ulcerogenic effect of Withaferin A. Intl J Integr Biol. 2009; 6(2):52–6.

Ahmed F, Hossain MH, Rahman AA, Shahid IZ. Antinociceptive and sedative effects of the bark of Cerbera odollam Gaertn. Adv Tradit Med.2006; 6(4):344–8. https://doi.org/10.3742/OPEM.2006.6.4.344 DOI: https://doi.org/10.3742/OPEM.2006.6.4.344

Ferdous M, Rouf R, JA, Uddin SJ. Short communication antinociceptive activity of the ethanolic extract of Ficus racemosa Lin. (Moraceae). Orient Pharm Exp Med. 2008; 8(1):93–6. https://doi.org/10.3742/OPEM.2008.8.1.093 DOI: https://doi.org/10.3742/OPEM.2008.8.1.093

Narayana KR, Reddy MS, Chaluvadi MR, Krishna DR. Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potential. Indian J Pharmacol. 2001 Feb; 33(1):2–16

Ramesh M, Rao YN, Rao AA, Prabhakar MC, Rao CS, Muralidhar N, Reddy BM. Antinociceptive and anti-inflammatory activity of a flavonoid isolated from Caralluma attenuata. J Ethnopharmacol. 1998; 62(1):63– 6. https://doi.org/10.1016/S0378-8741(98)00048-8 DOI: https://doi.org/10.1016/S0378-8741(98)00048-8

Uche FI, Aprioku JS. The phytochemical constituents, analgesic and anti-inflammatory effects of methanol extract of Jatropha curcas leaves in Mice and Wister albino rats. Journal of applied science and environmental management. JASEM. 2008; 12(4). https://doi.org/10.4314/jasem.v12i4.55247 DOI: https://doi.org/10.4314/jasem.v12i4.55247

Ramprasath VR, Shanthi P, Sachdanandam P. Immunomodulatory and anti-inflammatory effects of Semecarpus anacardium L INN. nut milk extract in experimental inflammatory conditions. Biol Pharm Bull. 2006; 29(4):693–700. https://doi.org/10.1248/bpb.29.693. PMid:16595901 DOI: https://doi.org/10.1248/bpb.29.693

Razan MR, Rahman MM, Tahia F, Hossain MK, Rashid MA. Analgesic and antidiarrheal activities of leaf of Podocarpus neriifolius D. Don. Bangladesh J Pharmacol. 2016; 19(2):215–8. https://doi.org/10.3329/bpj.v19i2.29283 DOI: https://doi.org/10.3329/bpj.v19i2.29283