Chemical Composition and Evaluation of Methanol Leaf Extract of Combretum dolichopetalum on Body Weights and Haematological Indices of Phenylhydrazine Induced-anaemic Rats


  • College of Medical Sciences, Alex Ekwueme Federal University, Department of Physiology, Faculty of Basic Medical Sciences, Abakaliki, Ebonyi, Nigeria
  • College of Medicine, University of Nigeria, Department of Physiology, Faculty of Basic Medical Sciences, Enugu, Nigeria
  • Michael Okpara University of Agriculture Umudike, Department of Biochemistry, Umuahia, Abia, Nigeria
  • College of Medical Sciences, Alex Ekwueme Federal University, Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Abakaliki, Ebonyi, Nigeria
  • Alex Ekwueme Federal University, Department of Chemistry/Biochemistry, Faculty of Sciences, Abakaliki, Ebonyi, Nigeria
  • College of Medical Sciences, Alex Ekwueme Federal University, Department of Anatomy, Faculty of Basic Medical Sciences, Abakaliki, Ebonyi, Nigeria
  • College of Medicine, Ebonyi State University, Department of Physiology, Faculty of Basic Medical Sciences, Abakaliki, Ebonyi, Nigeria


Anaemia is a serious health challenge in developing countries. This study evaluated the chemical composition of Combretum dolichopetalum (CD) leaves, the effect of its methanol extract on the body weights and haematological indices of phenylhydrazine induced-anaemic rats. Fresh matured leaves of CD were collected, identified and authenticated. Phytochemicals, proximate compositions, mineral elements, anti-nutritional factors and Gas Chromatography-Mass Spectrometry (GC-MS) analysis were determined. A total number of twenty (20) mature inbred apparently healthy male albino rats of the Wistar strain (100-150 g) were used for this study. Anaemia was induced by administering phenylhydrazine to rats in Groups 2, 3 and 4 comprising of five rats per group. Group 1 (non-anaemic control) (n = 5) and Group 2 (anaemic control) received distilled water respectively while Groups 3 and 4 were administered 200 and 400 mg/kg of CD leaf extract orally using oropharyngeal cannula once per day for 28 days. Blood samples were obtained for haematological analysis using standard methods. The result showed a significant increase in Red Blood Cell (RBC), Packed Cell Volume (PCV) and haemoglobin concentration in Groups 3 and 4 compared with the anaemic group. Chemical analysis showed the presence of some phytochemicals, proximate compositions, mineral elements and anti-nutritional factors. GC-MS analysis showed the presence of nine (9) compounds. This study indicates that CD is nutritionally rich and contains some important bioactive principles that support its anti-anaemic properties.


Anaemia, Combretum dolichopetalum, Haematological Parameters, Mineral Elements, Phytochemicals, Proximate Compositions

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