Effect of Imidacloprid on Histopathological Alterations of Brain, Gills and Eyes in hatchling carp (Cyprinus carpio L.)

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  • Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana ,IN
  • Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana ,IN
  • Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana ,IN
  • Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana ,IN




Brain, Cyprinus carpio, Eye, Gills, Histopathology, Imidacloprid
Zoology, Toxicology, Aquaculture


Imidacloprid, a neonicotinoid pesticide is used globally to control insect pest in rural and urban area where it enters into and contaminate the aquatic ecosystem. The present study was designed to study the toxic impacts of imidacloprid on developing hatchlings of Cyprinus carpio, for which fertilized eggs of C. carpio were exposed to different sublethal concentration of imidacloprid and 48 hrs LC50 was calculated as 78 ppm. Three different sublethal concentration of imidacloprid were selected for further experimentation (7.8 ppm, 15.6 ppm and 23.4 ppm) along with control. Hatchlings developed from treated and control eggs were fixed in Bouins fixative to analyze the histopathological effects of imidacloprid on brain, eyes and gills. The findings depicted several histopathological degenerations in brain, eyes and gills including neuronal degeneration, necrosis, vacuolation, Pyknotic nuclei, fragmented nuclei, mononuclear infiltration, spongiosis, reduced retinal pigmentation, ruptured lens tissue, uplifted sclerotic layer, ruptured arterial wall and degenerated lamellae.


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How to Cite

Harkrishan, ., Saraf, P., Tyor, A. K., & Bhardwaj, J. K. (2020). Effect of Imidacloprid on Histopathological Alterations of Brain, Gills and Eyes in hatchling carp (<i>Cyprinus carpio</i> L.). Toxicology International, 27(1&amp;2), 70–78. https://doi.org/10.18311/ti/2020/v27i1&2/25386



Research Articles
Received 2020-05-23
Accepted 2020-07-08
Published 2020-10-01



Mathur SC. Future of Indian pesticides industry in next millennium. Pesticide Information. 1999; 24:9–23.

Abdou KA, Hend MAE. Epidemiology of pesticides in developing countries. Advances in Clinical Toxicology. 2018; 3:128–33. https://doi.org/10.23880/ACT-16000128

Edwards CA. Pesticide residues in soil and water. In: Edwards CA (ed) Environmental pollution by pesticides. London: Plenum Press; 1973. https://doi.org/10.1007/978- 1-4615-8942-6_12. PMid: 4124892

Murty AS. Toxicity of Pesticides to fish. Boca Raton:CRC Press Inc; 1986.

Hayes WJ, Laws ER. Handbook of pesticide toxicology. San Diego: Academic Press; 1991.

Koprucu K, Aydin R. The toxic effects of pyrethroid deltamethrin on the common carp (Cyprinus carpio L.) embryos and larvae. Pesticide Biochemistry and Physiology. 2004; 80:47–53. https://doi.org/10.1016/j. pestbp.2004.05.004

Sweilum MA. Effect of sublethal toxicity of some pesticides on growth parameters, haematological properties and total production of Nile tilapia (Oreochromis niloticus L.) and water quality of ponds. Aquaculture Research. 2006; 37:1079–89. https://doi.org/10.1111/j.1365-2109.2006.01531.x

Dutta HM, Arends D. Effects of endosulfan on brain acetylcholinesterase activity in juvenile bluegill sunfish. Environmental Research. 2003; 91:157–62. https://doi. org/10.1016/S0013-9351(02)00062-2

Sakr SA, Jamal ALSM. Fenvalerate Induced Histopathological and Histochemical Changes in the Liver of the Catfish Clarias gariepinus. Journal of Applied Sciences Research. 2005; 1:263–67.

Perez MR, Rossi AS, Bacchetta C, et al. In situ evaluation of the toxicological impact of a waste water effluent on the fish Prochilodus lineatus: Biochemical and histological assessment. Ecological Indicators. 2018; 84:345–53. https:// doi.org/10.1016/j.ecolind.2017.09.004

Hovda LR, Hooser SB. Toxicology of newer pesticides for use in dogs and cats. Veterinary Clinics of North America: Small Animal Practice. 2002; 32:455–67. https://doi. org/10.1016/S0195-5616(01)00013-4

Tomlin CDS. The Pesticide Manual, 12th Edition. Bracknel: British Crop Protection Council; 2000.

Tennekes HA, Sanchez-Bayo F. Time-dependent toxicity of neonicotinoids and other toxicants: Implications for a new approach to risk assessment. Journal of Environmental & Analytical Toxicology. 2011; 4:1–8. https://doi. org/10.4172/2161-0525.S4-001

Pearse AGE. Histochemistry: Theoretical and Applied (revised edition of A.G.E. Pearse). Awnsham and John Churchill, London; 1968.

Moraes BS, Loro VL, Glusczak L, Pretto A, Menezes C, Marchezan E, Machado SO. Effects of four rice herbicides on some metabolic and toxicology parameters of teleost fish (Leporinus obtusidens). Chemosphere. 2007; 68:1597– 1601. https://doi.org/10.1016/j.chemosphere.2007.03.006. PMid:17451783

Omitoyin BO, Ajani EK, Fajimi AO. Toxicity Gramoxone (paraquat) to juvenile African catfish, Clarias gariepinus (Burchell, 1822). American-Eurasian Journal of Agricultural & Environmental Sciences. 2006; 1:26–30.

Penrith ML, Robinson JTR. Selenium toxicosis with focal symmetrical poliomielomalacia in postweaning pigs in South Africa. Onderstepoort Journal of Veterinary Research. 1996; 63:171–9.

Machado MR, Fanta E. Effects of the organophosphorous methyl parathion on the branchial epithelium of a freshwater fish Metynnis roosevelti. Brazilian Archives of Biology and Technology. 2003; 46:361–72. https://doi. org/10.1590/S1516-89132003000300008

Sharbidide AA, Metkari V, Patode P. Effect of Diazinon on acetylcholinesterase activity and lipid peroxidation of Poecilia reticulata. Research Journal of Environmental Toxicology. 2011; 5:152–61. https://doi.org/10.3923/ rjet.2011.152.161

Das BK, Mukherjee SC. A histological study of carp (Labeo rohita) exposed to hexachlorocyclohexane. Vet Arh. 2000; 70:169–80.

Rahman MZ, Hossain Z, Mellah MFA, Ahmed GU. Effect of diazinon 60EC on Anabus testudineus, Channa punctatus and Barbades gomonotus. Naga: The ICLARM Quarterly. 2002; 25:8–11.

Das BK. Studies on the effect of some pesticides and common used chemicals on Indian major carp and their ecosystem. PhD Thesis, Orissa University of Agriculture and Technology, Bhubaneswar, Orissa, India; 1997.

Altinok I, Capkin E. Histopathology of rainbow trout exposed to sublethal concentrations of Methiocarb or Endosulfan. Toxicologic Pathology. 2007; 35:405– 10. https://doi.org/10.1080/01926230701230353. PMid:17455089

Santhakumar M, Balaji M, Ramudu K. Histopathological lesions in the brain of ateleost Anabas testudineus exposed to monocrotophos. Journal of Environmental Biology. 2000; 21:297–9.

Jindal R, Kaur M, Kaur J. Lead induced toxicopathic corneal lesions in Ctenopharyngodon idellus (Cuvier and Valenciennes). International Journal of Zoology Studies. 2017; 2:29–33.

Uppal RK, Johal MS, Sharma ML. Toxicological effects and recovery of the corneal epithelium in Cyprinus carpio communis Linn. exposed to monocrotophos: An scanning electron microscope study. Veterinary Ophthalmology. 2013; 18:214–20. https://doi.org/10.1111/vop.12134. PMid:24373492

Chang SW, Chi RF, Wu CC, Su MJ. Benzalkonium chloride and gentamicin cause a leak in corneal epithelial cell membrane. Experimental Eye Research. 2000; 71:3–10. PMid:10880271

Hejduk J, Svobodova Z. Acute toxicity of Carbamate-based Pesticides for fish. Acta Veterinaria Brno. 1980; 49:251–7. https://doi.org/10.2754/avb198049030251

Pain S. Pesticide causes cataracts in salmon. New Science. 1989; 123:30.

Ostaszewska T, Kossakowski MK, Czarnogorski M, Resiak G. Histopathological changes in Juvenile Carp, Cyprinus carpio (L.) continuously Exposed to Alkaline levels of pH, from Hatching. Archives of Polish Fisheries. 1999; 7:329– 42.

Selvanathan J, Vincent S, Nirmala A. Histopathological changes in freshwater fish Clarias batrachus (Linn.) exposed to mercury and cadmium. International Journal of Life science and Pharma Research. 2012; 3:1–11.

Natarajan GM. Effect of sublethal concentration of metasystox on selective oxidative enzymes, tissue respiration and haematology of the freshwater air-breathing fish, Channa striatus (Bleeker). Pesticide Biochemistry and Physiology. 1985; 21:194–8. https://doi.org/10.1016/0048- 3575(84)90054-3

Arellano JM, Storch V, Sarasquete C. Histological changes and copper accumulation in liver and gills of the Senegales sole, Solea senegalensis. Ecotoxicology and Environmental Safety. 1999; 44:62–72. https://doi.org/10.1006/ eesa.1999.1801. PMid:10499990

Babatunde MM, Oladimeji AA, Rafindadi AH. Histopathological changes in the gills, livers and brains of O. niloticus (Trewavas) exposed to paraquat in chronic bioassay. International Journal of Science and Technology. 2014; 4:1–10.

Dutta HM, Richmonds CR, Zeno T. Effects of diazinon on the gills of bluegill sunfish Lepomis macrochirus. Journal of Environmental Pathology, Toxicology, and Oncology. 1993; 12:219–27.

Thigargarajah A, Grizzle JM. Pathology of diethyl nitrosamine toxicity to the fish (Rivulus marmoratus). Journal of Environmental Pathology, Toxicology, and Oncology. 1985; 2:219–32.

Ahmadivand S, Farahmand H, Mirvaghefi AR, Eagderi S, Shokrpoor S, Rahmati-Holasoo H. Histopathological and haematological response of male rainbow trout (Oncorhynchus mykiss) subjected to butachlor. Veterinary Medicine – Czech. 2014; 59:433–9. https://doi. org/10.17221/7683-VETMED

Chinabul S, Limsuwan S, Kanchana K. Toxic and sublethal effects of dipterex on fresh water fishes. NACA, WP; 1987

Ufodike EBC, Omoregie E. Toxicity of Gammalin 20 and Actellic 25 EC to O niloticus. Acta Hydrobiol 1990;32:447- 55.

Ojha J, Rooj NC, Mittal AK, Munshi Datta JS. Light and scanning electron microscopic studies on the effect of biocidal plant sap on the gills of a hill stream fish, Garra lanta (Ham.). Journal of Fish Biology. 1989; 34:165–70. https://doi.org/10.1111/j.1095-8649.1989.tb03299.x

Karan V, Vitorovic S, Tutundzic V, Poleksic V. Functional enzymes activity and gill histology of carp after copper sulfate exposure and recovery. Ecotoxicology and Environmental Safety. 1998; 40:49–55. https://doi. org/10.1006/eesa.1998.1641. PMid:9626535