Microbial Production and Purification of Cellulase Enzyme on Waste as a Substrate
Keywords:Aspergillus niger, Cellulase, Fermentation, Purification.
AbstractThe cellulase is of industrial importance and there is a high demand to produce low cost cellulase. The cellulase production was carried out by Aspergillus niger on wastes like saw dust and wheat straw, as it is a need to reduce and reuse waste from the environment. The production was optimized at 72 hrs for saw dust and wheat straw with enzyme activity of 249.10 U/ml/min and 338.02 U/ml/min respectively. The produced enzyme was purified, the specific enzyme activity of Cellulase after precipitation at 80% (NH4)2SO4 was observed as 4.71 U/min/mg for saw dust and 6.91 U/min/mg for wheat straw respectively. After ion-exchange chromatography best specific enzyme activity was observed in saw dust at 0.6 M NaCl with 3498.04 U/min/mg and for wheat straw at 0.4 M NaCl with 1692.6 U/min/mg. The industrial importance is to produce cellulase at low cost which can be achieved using waste as substrate as well as reduces toxicity of waste.
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