Efficacy of Trans-2-Hydroxycinnamic Acid Against Trichlorfon-Induced Oxidative Stress in Wistar Rats
Keywords:Oxidative stress, trans-2-hydroxycinnamic acid, trichlorfon, Wistar rats
AbstractTrichlorfon is an organophosphate insecticide used to control cockroaches, crickets, silverfish, bedbugs, fleas, cattle grubs, flies, ticks, leaf miners, and leaf-hoppers. It is also used to treat domestic animals for control of internal parasites. Trans-2-hydroxycinnamic acid (T2HCA) is a hydroxyl derivative of cinnamic acid. The present study highlights trichlorofon-induced toxicity and the protective role of T2HCA in the liver, kidney, and brain of female Wistar rats. The rats were given a single dose of trichlorofon (150 mg / kg bw) and pre- and post-treatment T2HCA (50 mg / kg bw) for seven days. Trichlorofon enhanced oxidative stress in liver, kidney, and brain of the rats, which was evident from the elevation of lipid peroxidation (LPO). The reduced level of non-enzymatic antioxidant glutathione (GSH) also indicated the presence of an oxidative insult. The activity of enzymatic antioxidants like superoxide dismutase (SOD), catalase (CAT), glutathione-s-transferase (GST), glutathione reductase (GR), and glutathione peroxidase (GPx) was significantly decreased on trichlorfon administration. Pre and post treatment with T2HCA decreased the LPO level and increased SOD, CAT, GST, GR, GPx, and GSH in the brain, liver, and kidney. Trichlorfon-induced reduction in acelylcholinestrase was also ameliorated with T2HCA treatment. In conclusion, trichlorfon-mediated induction in the reactive oxygen species and disturbance in the antioxidant enzymes' defense system was moderately ameliorated by antioxidant trans-2-hydroxycinnamic acid.
Rice-Evans C, Halliwell B, Lunt GG. Free radicals and oxidative stress, environment, drugs and food additives. London: Portland Press; 1995.
Thomson WT. Insecticides, acaricides and avicides agricultural chemicals. Book I. Fresno, CA: Thomson Publications; 1986.
Hayes WJ, Laws ER. Classes of pesticides. Handbook of Pesticide Toxicology. Vol. 3. New York: Academic Press, Inc; 1991.
US Environmental Protection Agency. Oct 31. EPA chemical profile. Trichlorfon. Washington, DC: DHHS Publication No.(CDC)90-8416,1990
Occupational Health Services. MSDS for trichlorfon. Syracuse, NJ: OHS Inc; 1991.
Staples RE, Goulding EH. Dipterex teratogenicity in the rat, hamster, and mouse when given by gavage. Environ Health Perspect 1979;30:105-13.
Astroff AB, Freshwater KJ, Eigenberg DA. Comparative organophosphate-induced effects observed in adult and neonatal Sprague-Dawley rats during the conduct of multigeneration toxicity studies. Reprod Toxicol 1998;12:619-45.
Singh R, Sharma P. Hepatoprotective effect of curcumin on lindane-induced oxidative stress in male Wistar rats. Toxicol Int 2011;18:124-9.
Hoult JR, Payá M. Pharmacological and biochemical actions of simple coumarins: Natural products with therapeutic potential.Gen Pharmacol 1996;27:713-22.
Taubert D, Breitenbach T, Lazar A, Censarek P, Harlfinger S, Berkels R, et al. Reaction rate constants of superoxide scavenging by plant antioxidants. Free Radic Biol Med 2003;35:1599-607.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70-7.
Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 1984;21:130-2.
Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389-94.
Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130-9.
Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: Biochemical role as a component of glutathione peroxidase. Science 1973;179:588-90.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75.
Ellman GL, Courtney KD, Andres V Jr, Feather,-Stone RM. A new and rapid colorimetric determination of acelylcholinesterase activity. Biochem pharmacol 1961;7:88-95.
Büyükokuroğlu ME, Taysi S, Polat F, Göçer F. Mechanism of the beneficial effects of dantrolene sodium on ethanol-induced acute gastric mucosal injury in rats. Pharmacol Res 2002;45:421-5.
Yavuz T, Delibas N, Yildirim B, Altuntas I, Candir O, Cora A, et al.Vascular wall damage in rats induced by organophosphorus insecticide methidathion. Toxicol Lett 2005;155:59-64.
Bano M, Bhatt DK. Neuroprotective role of a novel combination of certain antioxidants on pesticide induced toxicity in cerebrum of mice. Res J Agri Bio Sci 2007;3:664-9.
Pompella A, Visvikis A, Paolicchi A, De Tata V, Casini AF. The changing faces of glutathione, a cellular protagonist. Biochem Pharmacol 2003;66:1499-503.
Dwivedi N, Bhutia YD, Kumar V, Yadav P, Kushwaha P, Swarnkar H, et al. Effects of combined exposure to dichlorvos and monocrotophos on blood and brain biochemical variables in rats. Hum Exp Toxicol 2010;29:121-9.
Mansour MK, El-Kashairy AA, Rashid MA, Kortem KM. Oxidative and biochemical alterations induced by profenofos insecticide in rats. Nat and Sci 2009;7:1-14.
Irshad M, Chaudhuri PS. Oxidant-antioxidant system: Role and significance in human body. Indian J Exp Biol 2002;40:1233-9.
Yarsan E, Cakin O. Efforts of dichlorvos on lipid peroxidation in mice on sub-acute and sub-chronic periods. Pestic Biochem Physiol 2006;86:106-7.
Sahoo A, Samanta L, Chainy GB. Mediation of oxidative stress in HCH-induced neurotoxicity in rat. Arch Environ Contam Toxicol 2000;39:7-12.
Fridovich I. Biological effects of the superoxide radicals. Arch Biochem Biophys 1986;287:1-11.
Sharma P, Singh R. Protective role of curcumin on lindane induced reproductive toxicity in male Wistar rats. Bull Environ Contam Toxicol 2010;84:378-84.
Kavalci C, Durukan P, Ozer M, Cevik Y, Kavalci G. Organophosphate poisoning due to a wheat bagel. Intern Med 2009;48:85-8.
Yadav CS, Kumar V, Suke SG, Ahmed RS, Mediratta PK, Banerjee BD. Propoxur-induced acelylcholine esterase inhibition and impairment of cognitive function: Attenuation by Withania somnifera. Indian J Biochem Biophys 2010;47:117-20.
Foley S, Navaratnam S, McGarvey DJ, Land EJ, Truscott TG, RiceEvans CA. Singlet oxygen quenching and the redox properties of hydroxycinnamic acids. Free Radic Biol Med 1999;26:1202-8.
Nardini M, D'Aquino M, Tomassi G, Gentili V, Di Felice M, Scaccini C. Inhibition of human low-density lipoprotein oxidation by caffeic acid and other hydroxycinnamic acid derivatives. Free Radic Biol Med 1995;19:541-52.
Jin CC, Fang D, Bo Z, Li Y, Zhong LL. Antioxidant activity of hydroxycinnamic acid derivatives in human low density lipoprotein: Mechanism and structure–activity relationship.Food Chem 2007;104:132-9.