A New Method for Determining Acute Toxicity in Animal Models

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Acute toxicity, lethal dose50, new method


Background: The intake of pharmacological substances by man has solely increased and this may be in the form of food, medicines and beverages, other industrial and household products. These substances are capable of eliciting chronic and acute toxicity, which may be mild or severe, depending upon their nature. Acute toxicity is defined as the unwanted effect(s) that occurs either immediately or at a short time interval after a single or multiple administration of such substance within 24 hours. The principal aim of this paper is to introduce a new method for testing toxicity, which if adopted, should produce more accurate and reproducible results using few animals. Materials and Methods: The proposed method is divided into three stages, with the outcome of each stage determining whether to terminate testing of proceed to the next stage. A confirmatory (confidence) test is used to validate the final test result. The method shows enormous advantages, which include the use of few animals, exploration of a wide range of doses, it is simple and inexpensive. Conclusion: Furthermore, accurate and reproducible result can be gotten through this method. We therefore recommend that the method should be considered for endorsement for the testing of acute toxicity by the regulatory bodies.


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How to Cite

Chinedu, E., Arome, D., & Ameh, F. S. (2018). A New Method for Determining Acute Toxicity in Animal Models. Toxicology International, 20(3), 224–226. Retrieved from https://www.informaticsjournals.com/index.php/toxi/article/view/21739



Original Research
Received 2018-08-10
Accepted 2018-08-10
Published 2018-08-10



Sperling F. Introduction to toxicity evaluation session. Environ Health Perspect 1979;32:259.

Walum E. Acute oral toxicity. Environ Health Perspect 1998;106 Suppl 2:497"‘503.

Akhila JS, Deepa S, Alwar MC. Acute toxicity studies and determination of median lethal dose. Curr Sci 2007;93:917"‘20.

Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol 1983;54:275"‘87.

Turner R. Acute toxicity: The determination of LD50. In: Screening Methods in Pharmacology. New York: Academic Press; 1965.p. 61"‘3.

Bruce RD. An up"‘and"‘down procedure for acute toxicity testing.Fundam Appl Toxicol 1985;5:151"‘7.

Organization for Economic Co"‘operation and Development (OECD).Guideline for the testing of chemicals 423. Documentation on acute oral toxicity and acute class method, 2001. Available from: http://www.oecd.org. [Last accessed on 2013 Apr 25].

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