Toxicological Evaluation of Pooled Selected Fractions of <i>Mimosa invisa</i> and Protective Effect of Herbal Decoction
Keywords:<i>Boerhaavia diffusa, Hygrophila auriculata, Mimosa invisa, Tribulus terrestris</i>
AbstractThe study was undertaken to evaluate the toxicity produced by the pooled selected fractions of Mimosa invisa in rabbits. An attempt was made to protect the animal from the toxicity using a decoction containing Hygrophila auriculata, Tribulus terrestris and Boerhaavia diffusa. Eighteen adult rabbits were divided into three groups of six animals each. Group I served as control. Group II received pooled fraction of M. invisa at 0.4 g/kg orally. Group III was administered with pooled fraction along with the decoction containing H. auriculata, T. terrestris and B. diffusa equivalent to 5 g/kg. The experiment was conducted for 20 days. Group II showed significant increase in biochemical parameters like alanine transaminase, aspartate transaminase, gamma glutamyl tranferase, creatine kinase, alkaline phosphatase, creatinine and urea, suggesting liver and kidney toxicity. Histopathological examination of the liver and kidney supported these findings. Heart also showed mild histopathological changes. Administration of decoction reversed the biochemical and histopathological changes, indicating the protective effect of decoction.
Rajan A, Manomohan CB, Valsala KV, Sreekumaran T, Lalitha CR, Ramachandran KM, et al. Experimental studies on the toxicity of the plant Mimosa invisa Mart var inermis Adelbert in calves. Kerala J Vet Sci 1986;17:91-8.
Snedecor GW, Cochran WG. Statistical Methods. 8th ed. Calcutta, India: Oxford and IBH Publishing Company; 1985. p. 534.
Alex PC, Rajankutty K, Valsala KV, Nair KN. Mimosa poisoning in a heifer. J Vet Anim Sci 1991;22:134-6.
Alikutty KM, Pillai NP. Mimosa poisoning in a buffalo. Indian J Vet Med 1992;17:59-60.
Flaoyen A, Bratberg B, Froslie A, Gronstol H, Langseth W, Mantle PG, et al. Nephrotoxicity in goats caused by dosing with water extract from stems of Narthecium ossifragum plants. Vet Res Commun 1997;21:499-506.
Flaoyen A, Bratberg B, Gronstol H. Nephrotoxicity in lamb apparently caused by experimental feeding with Narthecium ossifragum. Vet Res Commun 1995;19:75-9.
Burtis CA, Ashwood ER. Tietz Fundamentals of Clinical Chemistry. 4th ed. London: W.B. Saunders Company; 1996. p. 881.
Hewawasam RP, Jayatilaka KA, Pathirana C, Madduwa LK. Protective effect of Asteracantha longifolia extract in mouse liver injury induced by carbon tetrachloride and paracetamol. J Pharm Pharmacol 2003;55:1413-8.
Craig AM, Pearson EG, Mayer C, Schmitz. Clinicopathological studies of tansy ragwort toxicosis in ponies: Sequential serum and histopathological changes. J Equine Vet Sci 1991;11: 261-2.
Flaoyen A, Hove K, Wilkins AL. Tolerance to nephrotoxic component of Narthecium ossifragum in sheep: The effects of repeated oral doses of plant extracts. Vet Res Commun 2001;25:127-36.