Experimental Hepatotoxicity Produced by Ethinyl estradiol

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  • Veterinary/Animal Husbandry Department, Government of MP, Jabalpur Division, Jabalpur, MP, 482002 ,IN
  • Departments of Pharmacology, NSCB Medical College, Jabalpur 482 003 ,IN
  • Departments of Zoology and Biotechnology, NES College of Science and Commerce, Jabalpur ,IN


Ethinyl estradiol, female albino rats, hepatotoxicity, histopathological study


Ethinyl estradiol (EO) is the most commonly used as a component of oral contraceptive and hormonal replacement therapy (HRT) in women. However, its excessive and prolonged use may cause cytotoxicity, including cancer of many organs. Hence, the present study was performed to produce the experimental hepatotoxicity in female albino rats. EO was administered to different groups of rats, respectively @ 250, 500 and 750 μg/kg body weight, orally, weekly for 16 and 20 weeks. One group of rats was administered with saline alone to serve as control. The rats were sacrificed after their respective experimental periods, and the livers were collected and preserved in 10% buffered formalin. Later on, the histopathological study of liver tissues was done. On the 17th week, the hepatic tissues showed severe congestion, focal areas of hemorrhage, extreme vacuolation of cytoplasm, distended sinusoids with dilated central veins. Degeneration and necrosis of hepatocytes as evidenced by increased cytoplasmic granularity, and dissolution of nuclear materials were seen. On the 21st weeks, these changes were extremely severe and quite conspicuous. Distinct fibrosis was also noticed. EO caused hepatotoxicity, the extent and severity of which were dose and time dependent, indicating that this drug at higher dose after prolonged duration (500 or 750 μg/kg, orally, weekly for 20 weeks) may cause the standard experimental hepatotoxicity in rats.


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How to Cite

Pandey, G., Pandey, S. P., & Sharma, M. (2018). Experimental Hepatotoxicity Produced by Ethinyl estradiol. Toxicology International, 18(2), 160–162. Retrieved from https://www.informaticsjournals.com/index.php/toxi/article/view/21268
Received 2018-05-18
Accepted 2018-05-18
Published 2018-05-18



Loose DS, Stancel GM. Estrogens and progestins. In: Brunton LL, editor. Goodman and Gilman's the pharmacological basis of therapeutics. 11th ed. New York: McGraw-Hill Co; 2006. p. 1541-71.

Sharma M. Studies on estrogen induced uterine and ovarian carcinogenesis and effect of roImmu in rat [PhD thesis]. Jabalpur, India: RDVV; 2008.

Sharma M, Pandey G. Effect of ProImmu, a herbal drug on estrogen caused uterine and ovarian cytotoxicity. Biomed 2010;5:57-62.

Sharma M, Pandey G, Khanna A. estrogen induced uterine damage in rats. Toxicol Int 2009;161:5-7.

Pandey G, Sharma M. Median lethal dose, acute and chronic toxicities of ethinyl estradiol estrogen. Natl J Life Sci 2008;52: 291-4.

Liehr JG. Genotoxicity of the steroidal estrogens oestrone and estradiol: possible mechanism of uterine and mammary cancer development. Hum Reprod Update 2001;7:273-81.

Estrogen and cancer. Available from: http://www.womenshealth.com [Last cited on 2006].

Pandey G, Sharma M. Efficacy of OptiLiv, a herbal formulation against estrogen induced liver damage in female rats. Int J Green Pharm 2007;1:39-41.

Pandey G, Sharma M, Pandey SP, Shrivastav AB. Hepatic tissue regeneration by OptiLiv in estrogen induced hepatotoxicity. Ind Res Comm 2008;2:47-52.

Culling CF. Hand book of histological techniques. 2nd ed. London: Butterworth and Co Ltd; 1963. p. 25-172.

Sheth AR, Adarkar MA, Rao SS, Virkar KD, Kora S. Liver function tests in women using oral contraceptives. Indian J Med Res 1967;55:1205-12.

Gayathri R, Priya DK, Gunassekaran GR, Sakthisekaran, D. Ursolic acid attenuates oxidative stress-mediated hepatocellular carcinoma by diethylnitrosamine in male Wistar rats. Asian Pac J Cancer Prev 2009;10:933-8.