Toxic effects of sub-chronic oral exposure of indoxacarb on biochemical parameters in buffalo calves
Keywords:Buffalo calves, indoxacarb, insecticides, sub-chronic toxicity
AbstractRepeated oral administration of Indoxacarb, an oxadiazine insecticide, at the dose rate of 1 mg/kg/day for 90 consecutive days produced mild signs of toxicity in buffalo calves. Indoxacarb significantly elevated the plasma levels of aspartate aminotransferase (38.3%), alanine aminotransferase (52.9%), alkaline phosphatase (29.5%) and acid phosphatase (36.6%). Cholinesterase levels in plasma, whole blood and erythrocyte also showed elevation. Indoxacarb did not produce any significant alterations in levels of blood urea nitrogen and creatinine. However there was an increase in blood glucose (16.9%), plasma cholesterol (19.6%) and total plasma proteins (10.8%). It can be concluded from the present study that indoxacarb is a low risk insecticide in buffalo calves.
Brar, RS, Sandhu, HS and Singh, A (2000). Veterinary Clinical Diagnosis by Laboratory Methods. Kalyani Publishers, Ludhiana- New Delhi.
Cohn, C and Kaplan, A (1971). Clinical blood chemistry. In: Textbook of Clinical Pathology, 8th edn. (Eds: Miller S E and Weller J M) pp, 235-80. The Williams and Wilkins Company, Baltimore, U.S.A.
Cornelius, CE (1989). Liver functions. In: Clinical Biochemistry of Domestic Animals. (Ed) Kaneko J J. pp. 364-397, Academic Press Inc, San Diego.
Dinman, BD, Hamdi, EA, Fox, CF and Frazola, WJ (1963). Carbon tetrachloride toxicity: Hepatostructural and enzymatic changes. Arch. Environ. Hlth., 7: 630-646.
Dinter, A and Wiles, JA (2000). Safety of the new DuPont insecticide "Indoxacarb” to beneficial arthropods: an overview, Bull. OILB/ SROP, 23: 149–156.
Frankel, S, Reitmen, S and Sonnerwirth, AC (1970). Gradhiwhol's Clinical Laboratory Methods and Diagnosis. Vol., pp. 82-83, The C.V. Mosby Co.,St. Louis.
Harder, HH, Riley, SL, McCann, SF and Sherrod, DW (1997). DPXMP062: A novel broad-spectrum, environmentally soft, insect control compound, Proceedings Beltwide Cotton Conferences Vol. 1, New Orleans, LA pp. 48– 50.
Kaplan, MM and Righetti, A (1970). Introduction of rat liver alkaline phosphatase, the mechanism of serum elevation in bile duct obstruction. J. Clin. Invest. 49: 508-16.
Lapied, B, Grolleau, F and Sattelle, DB (2001). Indoxacarb, an oxadiazine insecticide, blocks insect neuronal sodium channels. British J. Pharmacol., 132: 587-595.
Moroi, K, Ushaya, S, Satoh, T and Kuga, T (1976). Enzyme induction by repeated administration of tetrachlorvinphos in rats. Toxicol. Appl. Pharmacol., 37: 162167.
National Registration Authority for Agricultural and Veterinary Chemicals (2000). ISSN 1443- 1335, Public release Summary on evaluation of the new active indoxacarb in the product Dupont Steward Insecticide: Canberra Australia.
Oser, BL (1976). Hawk's Physiological Chemistry. McGrawHill Book Company, New York, London.
Ramazzotto, LJ and Carlin, R (1978). Effects of DMSO on SGOT during hypothermia in adrenalectomized rats. Life Sci., 22: 329-336.
Reinhold, JG (1953). Total Proteins. In: Standard Methods of Clinical Chemistry, Vol. 1(Ed: Reiner M) pp.88.
Academic Press, New York.
Seetharam, S, Sussman, NL, Komoda, T and Alpers, DH (1986). The mechanism of elevated alkaline phosphatase activity after duct ligation in rats. Hepatol., 6: 374-380.
Snedecor, GW and Cochran WG (1967). Statistical methods, 6th edn, New Delhi, Oxford and I B H, Calcutta.
Tennant, BC (1997). Hepatic fuction. In: Clinical Biochemistry of Domestic Animals. 5th edn (Eds: Kaneko J J, Harvey J W and Bruss M L) pp, 327-352, Academic Press, San Diego.
Voss, G and Sachse, K (1970). Red cell and plasma cholinesterase activities in microsamples of human and animal blood determined simultaneously by a modified acetylcholie/DTNB procedure. Toxicol. App.
Pharmacol., 16: 764-842.
Welch, WJ ( 1993). How cells respond to stress. Scientific American 268(5): 34-41.
Wilson, R, Doell, BH, Groger, W, Hope, J and Gallatey, JB (1970). The physiology of liver enlargement. In: Metabolic Aspects of food Safety. Roe F J C (Ed). Blackwell Scientific Pub., Oxford. pp 363.
Wing, KD, Schnee, ME, Sacher, M and Connair, M (1998). A novel oxadiazine insecticide is bioactivated in lepidopteran larvae. Arc. of Insect Biochem. and Physiol., 37: 91-103.
Wing, KD, Sacher, M, Kagaya, Y, Tsurubuchi, Y, Mulderig, L, Connair, M and Schnee, M (2000). Bioactivation and mode of action of the oxadiazine indoxacarb in insects. Crop Protect., 19: 537-545
Wotton, IDP (1964). Microanalysis in Medical Biochemistry. J and A Churchill Ltd., London.
Zhao, X, Nagata, K, Marszalec, W, Yeh, JZ and Narahashi, T (1999). Effects of the oxadiazine insecticide indoxacarb, DPX- MPO62, on neuronal nicotinic acetylcholine receptors in mammalian neurons, Neurotoxicol., 20(4): 70
Zlotkin, E (2001). Insecticides Affecting Voltage-Gated Ion Channels. In: Biochemical Sites Important to Insecticide Action and Resistance; Ishaaya, I., Ed.; SpringerVerlag: Berlin; 43–76.