A study on free radical-induced renal toxicity due to cyclophosphamide and its amelioration with N-acetyl cysteine

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Cyclophosphamide (CYP), N-acetyl cysteine (NAC), Oxidative stress


A study was conducted to evaluate the protective effect of N-acetyl cysteine (NAC) against experimentally-induced renal toxicity due to cyclophosphamide (CYP) in rats. Three groups (2, 3 and 4) of female Wistar Kyoto rats with 6 animals in each group were administered with CYP @ 40 mg/kg bwt on 1st, 3rd, 5th and 7th day by intraperitoneal route. Group 2 remained as toxic control. Group 3 received NAC from 1st to 14th day @ 0.2% of feed and group 4 was treated with NAC from 8th to 14th day. Group 1 was maintained on basal diet for 14 days. The study revealed a significant (P < 0.05) increase in the concentration of serum creatinine, blood urea nitrogen, thiobarbituric acid reacting substances (TBARS) and protein carbonyls, and a decrease in the reduced glutathione (GSH) concentration of kidney. These results are suggestive of CYP-induced oxidative stress resulting in renal damage. Feeding of NAC could not offer protection against CYPinduced renal damage in this study.


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Jyothi, K., Reddy, A. G., K. S., G., Kumar, B. A., & Reddy, G. D. (2018). A study on free radical-induced renal toxicity due to cyclophosphamide and its amelioration with N-acetyl cysteine. Toxicology International, 16(2), 137–139. Retrieved from https://www.informaticsjournals.com/index.php/toxi/article/view/20872



Original Research



Balasubramanian, KA, Manohar, M and Mathan, VI (1988). An unidentified inhibitor of lipid peroxidation in intestinal mucosa. Biochim Biophysic Acta, 962: 51-58.

Dekhuijzen, PNR (2004). Antioxidant properties of Nacetylcysteine: their relevance in relation to chronic

obstructive pulmonary disease. European Respiratory J., 23: 629-636.

Flora De S, Izzotti, A, D' Agostini, F and Balansky (2001). Mechanisms of N-acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking – related end – points. Carcinogenisis, 22: 999 – 1013.

Georgia, JP, Dianne, C, Michael, B, Sally, L, Eric, W, Brian, H, Colin, JH and Roland, CW (2005). Role of hepatic cytochrome P450s in the pharmacokinetics and toxicity of cyclophosphamide: Studies with the hepatic cytochrome P450 reductase Null mouse. Cancer Res., 65: 4211-4217.

Henry, SF, Colvin, OM, Aisaka, K, Jim, P, Bossen, EH, Reimer, KA, Powell, JB, John H, Gross, SS, Roberto, L, Bigner, DD and Griffith, OW (1990). Glutathione protects cardiac and skeletal muscle from cyclophosphamide-induced toxicity. Cancer Res., 50: 2455-2462.

Kondala, RA, John, J, Leonard, AH and Leonore, AH (2007). N-Acetylcysteine - a safe antidote for cysteine/glutathione deficiency. Current Opinion in Pharmacol., 7: 355-359.

Levine, RL, Garland, D, Oliver, CN, Amici, A, Climent, I, Lenz, AG, Ahn, BW, Shaltiel, S and Stadtman, ER (1990). Determination of carbonyl content in oxidatively modified proteins. Methods in Enzymol., 186: 464-478.

Moore, MJ (1991). Clinical Pharmacokinetics of cyclophosphamide. Clinical Pharmacokinetics, 20: 194-208.

Moron, MS, Depierre, JW and Mannervik, B (1979). Levels of glutathione, glutathione reductase and glutathione S transferase in rat lung and liver. Biochim Biophysic Acta, 582: 67-68.

Senthilkumar, S, Devaki, T, Manohar, BM and Babu, MS (2006). Effect of squalene on cyclophosphamideinduced toxicity. Clinica Chimica Acta, 364: 335-342.

Sudharsan, PT, Mythili, Y, Selvakumar and Varalakshmi, P (2006): Lupeol and its ester inhibit alteration of myocardial permeability in cyclophosphamide administered rats. Mol. Cell Biochem., 292: 39-44.

Tepel, M, van der Giet, M and Schwarzfeld, C (2000). Prevention of radiographic-contrast-agent-induced reductions in renal function by acetylcysteine. New Engl J, Med., 343: 180-184.

Zhang, S, Chai, FY, Yan, HGY and Harding, JJ (2008). Effects of N-acetylcysteine and glutathione ethyl ester drops on streptozotocin-induced diabetic cataract in rats. Mol Vision, 14: 862-870.

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