Single dose neurotoxicity screening studies of insecticide combination (Cypermethrin and Profenofos) in Wistar rats
Keywords:Cypermethrin, Profenofos, FOB, Neurotoxicity, Neurobehaviour, Motor activity
AbstractThe present investigation has been taken up to determine the toxic effects of combination insecticide (Profenofos 40% + Cypermethrin 4% EC) on nervous system of rats. A single oral dose of test substance was administered to five rats per sex at a concentration of 25, 75 and 225mg/kg body weight to experimental animals. The animals were observed under carefully standardized conditions with sufficient frequency to ensure the detection and quantification of behavioral and/ or neurologic abnormalities. In addition, serum cholinesterase was estimated after 24 hours of dosing. Motor activity of individual animal was measured using an automated device. Perfused (in-situ) nervous tissues were examined microscopically. The test article at the dose level of 75 and 225 mg/kg induced treatment related and dose dependent neurobehavioural alterations in rats of either sex. However, observed alterations were reversible, further no structural/ histopathological alterations were apparent. Single dose of test article at 25 mg/kg did not reveal any toxic signs or neurobehavioral alterations hence, it is considered to be the No Observed Adverse Effect Level (NOAEL).
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