Camphora Augments Humoral Mediated Immunity and Decreases Delayed type Hypersensitivity in BALB/c Mice

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Authors

  • Drug Standardization Department, Dr Anjali Chatterji Regional Research Institute for Homeopathy, Under Central Council for Research in Homeopathy, Kolkata - 700035, West Bengal ,IN
  • Drug Standardization Department, Dr Anjali Chatterji Regional Research Institute for Homeopathy, Under Central Council for Research in Homeopathy, Kolkata - 700035, West Bengal ,IN
  • Drug Standardization Department, Dr Anjali Chatterji Regional Research Institute for Homeopathy, Under Central Council for Research in Homeopathy, Kolkata - 700035, West Bengal ,IN
  • Drug Standardization Department, Central Council for Research in Homeopathy, New Delhi - 110058 ,IN
  • Drug Standardization Department, Dr D P Rastogi Central Research Institute for Homeopathy, Under Central Council for Research in Homeopathy, Noida - 201301, Uttar Pradesh ,IN

DOI:

https://doi.org/10.18311/jnr/2023/31420

Keywords:

Camphora, Cell-mediated Immunity, Delayed Type Hypersensitivity, Homeopathic Medicine, Humoral Immunity, Immunomodulation

Abstract

Background: Immunomodulation encompasses all therapeutic interventions aimed at modifying the immune response. The immune response augments are desirable to prevent infection in immunodeficiency states and fight established diseases. In this context, the present study investigated the effect of the homeopathic medicine, Camphora, in 6CH, 30CH, and 200CH potencies on immunomodulation in experimental animals. The acute oral toxicity study was also carried out in 6CH, 30CH, and 200CH potencies to determine the safe dose volume for the immunomodulatory study. Methodology: Acute oral toxicity studies of Camphora in 6CH, 30CH, and 200CH potencies were carried out as per OECD guideline 423 with slight modifications in Wistar albino rats. Humoral immunity, i.e., primary and secondary humoral responses, was assessed by measuring the hemagglutination titre of sheep red blood cells. Delayed-Type Hypersensitivity (DTH) was evaluated by measuring footpad thickness in BALB/c mice. Results: Camphora in 6CH, 30CH and 200CH potencies at a dose volume of 2000 µl/kg did not cause any mortality in the rats when administered as a single dose. Camphora in 6CH, 30CH and 200CH potencies showed augmented primary and secondary humoral responses against the SRBC antigen in BALB/c mice. However, the values were statistically non-significant except in the case of 6 CH potency (p<0.01), which showed statistically significant primary anti-SRBC antibodies. In the DTH assay, Camphora in 6CH, 30CH and 200CH potencies significantly decreased the paw volume ratio after 24 hrs of SRBC injection in the paw, thus insinuating its role in reducing cell-mediated immunity. Camphora in 6CH, 30CH and 200CH potencies also showed enhanced antibody titres and decreased paw volume compared to vehicle control, i.e. dispensing alcohol, suggesting that the effect was imminent because of Camphora. Conclusion: The study results indicate that Camphora in 6CH, 30CH, and 200 CH potencies is safe up to a dose volume of 2000 µl/kg when administered as a single dose, augments the primary and secondary humoral immunity, and decreases DTH in experimental animals. The current study’s findings suggest that Camphora might be useful as an immunomodulator in treating immune system disorders and infectious diseases and require further investigation to investigate its mechanism of action.

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Published

2023-03-23

How to Cite

Saka, V. P., Kumar, G. V. N., Goswami, A., Verma, D., & Gupta, P. (2023). <i>Camphora</i> Augments Humoral Mediated Immunity and Decreases Delayed type Hypersensitivity in BALB/c Mice. Journal of Natural Remedies, 23(1), 157–167. https://doi.org/10.18311/jnr/2023/31420

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Section

Research Articles
Received 2022-10-10
Accepted 2023-01-09
Published 2023-03-23

 

References

Kajaria D, Tripathi J, Tiwari S, Pandey B. Immunomodulatory effect of ethanolic extract of Shirishadi compound. AYU (An Int. Q. J. Res. Ayurveda). 2013; 34:322. https://doi. org/10.4103/0974-8520.123136 DOI: https://doi.org/10.4103/0974-8520.123136

Abood WN, Fahmi I, Abdulla MA, Ismail S. Immunomodulatory effect of an isolated fraction from Tinospora crispa on intracellular expression of INF-γ, IL-6 and IL-8. BMC Complement. Altern. Med. 2014; 14:205. https://doi.org/10.1186/1472- 6882-14-205 DOI: https://doi.org/10.1186/1472-6882-14-205

Yuandani, Jantan I, Rohani AS, Sumantri IB. Immunomodulatory Effects and Mechanisms of Curcuma Species and Their Bioactive Compounds: A Review. Front. Pharmacol. 2021; 12. https://doi. org/10.3389/fphar.2021.643119 DOI: https://doi.org/10.3389/fphar.2021.643119

Acharya D, Shrivastava A. Indigenous herbal medicines. (Aavishkar Publishers, Distributors, 2008).

Melchart D, et al. Results of Five Randomized Studies on the Immunomodulatory Activity of Preparations of Echinacea. J. Altern. Complement. Med. 1995; 1:145-160. https://doi.org/10.1089/acm.1995.1.145 DOI: https://doi.org/10.1089/acm.1995.1.145

Bellavite P, Ortolani R, Conforti A. Immunology and Homeopathy. 3. Experimental Studies on Animal Models. Evidence-Based Complement. Altern. Med. 2006; 3:171-186. https://doi.org/10.1093/ecam/ nel016 DOI: https://doi.org/10.1093/ecam/nel016

Kishore L, Singh R. Ameliorative effect of Cephalandra indica homeopathic preparation in STZ induced diabetic nephropathy rats. J. Ayurveda Integr. Med. 2019; 10:255-261. https://doi.org/10.1016/j. jaim.2017.07.010

Sarkar A, Datta P, Das AK, Gomes A. Anti-rheumatoid and anti-oxidant activity of homeopathic Guaiacum officinale in an animal model. Homeopathy. 2014; 103:133-138. https://doi.org/10.1016/j. homp.2013.08.006 DOI: https://doi.org/10.1016/j.homp.2013.08.006

Kishore L, Singh R. Ameliorative effect of Cephalandra indica homeopathic preparation in STZ induced diabetic nephropathy rats. J. Ayurveda Integr. Med. 2019; 10:255-261. https://doi.org/10.1016/j. jaim.2017.07.010 DOI: https://doi.org/10.1016/j.jaim.2017.07.010

1Lamba C, et al. Evaluation of homoeopathic treatment in polycystic ovary syndrome: A single blind, randomised, placebo-controlled pilot study. Indian J. Res. Homoeopath. 2018; 12:35. https://doi. org/10.4103/ijrh.ijrh_18_18 DOI: https://doi.org/10.4103/ijrh.ijrh_18_18

Jyothilakshmi V, et al. Beneficial Antioxidative Effect of the Homeopathic Preparation of Berberis vulgaris in Alleviating Oxidative Stress in Experimental Urolithiasis. Res. Complement. Med. 2014; 21:7-12. https://doi.org/10.1159/000360240 DOI: https://doi.org/10.1159/000360240

Hanif K, Kumar M, Singh N, Shukla R. Effect of homeopathic Lycopodium clavatum on memory functions and cerebral blood flow in memory impaired rats. Homeopathy. 2015; 104:24-28. https:// doi.org/10.1016/j.homp.2014.08.003 DOI: https://doi.org/10.1016/j.homp.2014.08.003

Kundu T, Shaikh A, Jacob S. To evaluate the role of homoeopathic medicines as add-on therapy in patients with rheumatoid arthritis on NSAIDs: A retrospective study. Indian J. Res. Homoeopath. 2014; 8:24. https://doi.org/10.4103/0974-7168.129674 DOI: https://doi.org/10.4103/0974-7168.129674

Kundu N, et al. Homeopathic Treatment of Rheumatoid Arthritis: An Open, Observational Trial. Homœopathic Links. 2019; 32:216-223. https://doi. org/10.1055/s-0039-3402080 DOI: https://doi.org/10.1055/s-0039-3402080

Vo HW, Michalsen A, Brunjes R. Efficacy and tolerability of a complex homeopathic drug in children suffering from dry cough - A double-blind, placebo controlled, clinical trial. Drug Res. (Stuttg). 2018; 68:444-449. https://doi.org/10.1055/s-0043-124179 DOI: https://doi.org/10.1055/s-0043-124179

Gibson R, Gibson S, MacNeill A, Buchanan W. Homoeopathic therapy in rheumatoid arthritis: evaluation by double‐blind clinical therapeutic trial. Br. J. Clin. Pharmacol. 1980; 9:453-459. https://doi. org/10.1111/j.1365-2125.1980.tb05840.x DOI: https://doi.org/10.1111/j.1365-2125.1980.tb05840.x

Nahar K. Homoeopathy and immunomodulation: A narrative review article. Int. J. Homoeopath. Sci. 2021; 5:246-249. https://doi.org/10.33545/26164485.2021. v5.i2d.391 DOI: https://doi.org/10.33545/26164485.2021.v5.i2d.391

Singh R, Jawaid T. Cinnamomum camphora (Kapur): Review. Pharmacogn. J. 2012; 4:1-5. https://doi. org/10.5530/pj.2012.28.1 DOI: https://doi.org/10.5530/pj.2012.28.1

Ministry of Health and Family Welfare(India). Homoeopathic Pharmacopoeia of India. Govt. of India. 1990; 1:1-234.

Ministry of AYUSH. Essential Drug List Homeopathy. 2013.

Clarke JH. Materia Medica. 1902.

Takacs M, et al. Adjunctive homeopathic treatment of hospitalised COVID-19 patients (COVIHOM): A retrospective case series. Complement. Ther. Clin. Pract. 2021; 44:101415. https://doi.org/10.1016/j. ctcp.2021.101415 DOI: https://doi.org/10.1016/j.ctcp.2021.101415

Chen W, Vermaak I, Viljoen A. Camphor - A Fumigant during the Black Death and a Coveted Fragrant Wood in Ancient Egypt and Babylon - A Review. Molecules. 2013; 18:5434-5454. https://doi. org/10.3390/molecules18055434 DOI: https://doi.org/10.3390/molecules18055434

Philpott NW. Intramuscular Injections of Camphor in the Treatment of Engorgement of the Breasts. Can. Med. Assoc. J. 1929; 20:494-5.

Lee HJ, et al. In vitro anti-inflammatory and anti oxidative effects of Cinnamomum camphora extracts. J. Ethnopharmacol. 2006; 103:208-216. https://doi. org/10.1016/j.jep.2005.08.009 DOI: https://doi.org/10.1016/j.jep.2005.08.009

Xiao S, et al. The anti-inflammatory potential of Cinnamomum camphora (L.) J. Presl essential oil in vitro and in vivo. J. Ethnopharmacol. 2021; 267:113516. https://doi.org/10.1016/j.jep.2020.113516 DOI: https://doi.org/10.1016/j.jep.2020.113516

Bespalov A, Wicke K, Castagne V. Blinding and Randomization. Handb. Exp. Pharmacol. 2020; 257:81-100. https://doi.org/10.1007/164_2019_279 DOI: https://doi.org/10.1007/164_2019_279

Al-Afifi NA, Alabsi AM, Bakri MM, Ramanathan A. Acute and sub-acute oral toxicity of Dracaena cinnabari resin methanol extract in rats. BMC Complement. Altern. Med. 2018; 18:50. https://doi. org/10.1186/s12906-018-2110-3 DOI: https://doi.org/10.1186/s12906-018-2110-3

Puri A, et al. Immunostimulant Agents from Andrographis paniculata. J. Nat. Prod. 1993; 56:995- 999. https://doi.org/10.1021/np50097a002 DOI: https://doi.org/10.1021/np50097a002

Singh S, et al. Role of homoeopathic mother tinctures in rheumatoid arthritis: An experimental study. Indian J. Res. Homoeopath. 2015; 9:42. https://doi. org/10.4103/0974-7168.154348 DOI: https://doi.org/10.4103/0974-7168.154348

Singh S, et al. Attenuation of Complete Freund’s Adjuvant-induced arthritis by different dilutions of Eupatorium perfoliatum and Crotalus horridus and their safety evaluation. Indian J. Res. Homoeopath. 2019; 13:107-117. https://doi.org/10.4103/ijrh. ijrh_23_19 DOI: https://doi.org/10.4103/ijrh.ijrh_23_19

Puri A, et al. Immunostimulant activity of Nyctanthes arbor-tristis L. J. Ethnopharmacol. 1994; 42:31-37. https://doi.org/10.1016/0378-8741(94)90020-5 DOI: https://doi.org/10.1016/0378-8741(94)90020-5

Gokhale A, Damre A, Saraf M. Investigations into the immunomodulatory activity of Argyreia speciosa. J. Ethnopharmacol. 2003; 84:109-114. https://doi. org/10.1016/S0378-8741(02)00168-X DOI: https://doi.org/10.1016/S0378-8741(02)00168-X

Percie du Sert N, et al. The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. PLOS Biol. 2020; 18:e3000410. https://doi. org/10.1371/journal.pbio.3000410 DOI: https://doi.org/10.1371/journal.pbio.3000410

Smith AJ, Clutton RE, Lilley E, Hansen KE. A. and Brattelid, T. PREPARE: guidelines for planning animal research and testing. Lab. Anim. 2018; 52:135- 141. https://doi.org/10.1177/0023677217724823 DOI: https://doi.org/10.1177/0023677217724823

World Health Organization. Safety issues in the preparation of homeopathic medicines. World Health Organization. 2009.

OECD. Test No. 423: Acute Oral toxicity - Acute Toxic Class Method. Oecd Guidel. Test. Chem. 2002; 1-14. https://doi.org/10.1787/9789264071001-en DOI: https://doi.org/10.1787/9789264071001-en

Janhavi P, Divyashree S, Sanjailal KP, Muthukumar SP. DoseCal: a virtual calculator for dosage conversion between human and different animal species. Arch. Physiol. Biochem. 2022; 128(2):426-430. https://doi. org/10.1080/13813455.2019.1687523 DOI: https://doi.org/10.1080/13813455.2019.1687523

Nfambi J, Bbosa GS, Sembajwe LF, Gakunga J, Kasolo JN. Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in Wistar albino rats. J. Basic Clin. Physiol. Pharmacol. 2015; 26. https://doi. org/10.1515/jbcpp-2014-0104 DOI: https://doi.org/10.1515/jbcpp-2014-0104

Kind TJ, Osborn BA, Goldsby RA. Kuby Immunology. (W.H. Freeman and Company, 2007).

Zaveri M, Gohil P, Jain S. Immunostimulant Activity of n-Butanol Fraction of Root Bark of Oroxylum indicum, vent. J. Immunotoxicol. 2006; 3:83-99. https://doi.org/10.1080/15476910600725942 DOI: https://doi.org/10.1080/15476910600725942

Bhat J, Kabelitz D. An introduction to immunology and epigenetics. Epigenetics of the Immune System (Taylor and Francis, 2020). https://doi.org/10.1016/ B978-0-12-817964-2.09991-3

Elgert KD. Immunology: understanding the immune system. Wiley. 2009.

Burlingham WJ, Jankowska-Gan E, VanBuskirk AM, Pelletier RP, Orosz CG. Delayed Type Hypersensitivity Responses in Measuring Immunity. Elsevier. 2005; 407-418. https://doi.org/10.1016/B978-012455900- 4/50297-X DOI: https://doi.org/10.1016/B978-012455900-4/50297-X

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