Host-insect and host-plant associated diversity in microbiota isolated from most important Oriental-Australian region egg parasitoid

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Authors

  • ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore – 24, Karnataka ,IN
  • ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 89, Karnataka ,IN
  • 1ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore – 24, Karnataka ,IN
  • 1ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore – 24, Karnataka ,IN
  • ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore – 24, Karnataka ,IN
  • ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore – 24, Karnataka ,IN
  • ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore – 24, Karnataka ,IN

DOI:

https://doi.org/10.18311/jbc/2017/18228

Keywords:

Egg parasitoid, evolutionary relationship, host-insects, host-plants, microbiota
Trichogrammatids

Abstract

Host-insect and host-plant associated differentiation of genetically divergent microbiota were recorded from economically important egg parasitoid collected from 26 locations in India, Trichogramma chilonis constituted 86.8% of the populations collected. It was recorded from 14 host-insects, 14 different crops and weed plants from 12 states. Nine species of yeast were recorded from parasitoid from 5 host-insects with Wickerhamomyces anamalus was isolated from 36.4% samples and highest numbers were recorded from parasitoid collected on sugarcane. Bacillus cereus, Pseudomonas sp. and Stenotrophomonas maltophilia from T. chilonis constitute 64.3% of bacterial diversity based on their 16S rDNA sequences. For taxonomic identification using 16s rDNA and ITS sequences, we performed taxonomic classification of total 33 ITS isolates against UNITE Fungal ITS database and assigned taxonomy hierarchy to the sequences. Also, a total of 13 isolates 16s rDNA sequences were taxonomically assigned against RDP 16s rDNA database using RDP Naive Bayesian rRNA Classifier Version 2.1. Most of the species are correctly identified in the respective species members with high confidence threshold value support.

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Author Biography

S. K. Jalali, ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore – 24, Karnataka

Principal Scientist

Division of Molecular Entomology

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Published

2018-04-06

How to Cite

Jalali, S. K., Sriram, S., Venkatesan, T., More, R. P., Navik, O., Lalitha, Y., & Ojha, R. (2018). Host-insect and host-plant associated diversity in microbiota isolated from most important Oriental-Australian region egg parasitoid. Journal of Biological Control, 31(4), 229–239. https://doi.org/10.18311/jbc/2017/18228

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Section

Research Articles
Received 2017-11-23
Accepted 2018-02-01
Published 2018-04-06

 

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