Evaluation of Herbal Neonatal Chick Care Against Iron-induced Toxicity in Broilers


  • College of Veterinary Science, Rajendranagar, Department of Pharmacology and Toxicology, Hyderabad, 500 030, India


A study was conducted to evaluate the protective effect of herbal Neonatal Chick care (NNCC) against iron-induced oxidative stress. A total of 130 day-old sexed male broiler chicks (Vencobb strain) were randomly divided into six groups consisting of 25 chicks each in groups 1–4 and 15 each in groups 5 and 6. Group 1 was maintained on basal diet, groups 2 and 3 on herbal NNCC at 6 and 8 g/chick/day, respectively, for 2 days immediately after hatching and later continued with basal diet up to 6 wk. Group 4 was given FeSO4 at 0.5% of feed for 6 wk, while groups 5 and 6 were given NNCC as in groups 2 and 3, and later continued with the FeSO4 as in group 4 for 6 wk. The concentration of thiobarbituric acid reactive substances (TBARS), protein carbonyls, glucose and calcium, and the activity of alanine transaminase (ALT) were significantly (P<0.05) increased in group 4 at the end of 6th week, while the concentration of reduced glutathione (GSH), and the activities of superoxide dismutase (SOD) and catalase, phytohemagglutinin (PHA) index and HI titer were significantly (P<0.05) decreased in group 4. The NNCC treated groups (2, 3, 5 and 6) showed marked improvement in all the above parameters. It can be concluded that herbal NNCC offered protection and proved beneficial in resisting the adverse effects of stressor.


Ferrous sulfate, herbal neonatal chick care, oxidative stress

Subject Discipline


Full Text:


Halliwell B. Reactive oxygen species and the central nervous system. J Neurochem 1992;59:1609-23.

Beard J. Iron deficiency alters brain development and functioning. J Nutrition 2003;133:14685-725.

Halliwell B, Gutteridge JM. Oxidative stress: Adaptation, damage, repair and death. Free Radicals in Biology and Medicine. 3rd ed. UK: Oxford University Press; 1999. p. 246-350.

Droge W. Oxidative stress and ageing. Adv Exp Med Biol 2003;543:191-200.

Farrukh A, Iqbal A, Zafar M. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turkish J Biol 2006;30:177-83.

Baba, Lisa MS, McGrath RN, Jacqueline M, Faan RN. Oxygen free radicals: effects in the newborn period. Adv Neonatal Care 2008;8:256-64.

Madesh M, Balasubramanian KA. Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J Biochem Biophys 1998;35:184-8.

Asru KS. Colorimetric assay of catalase. Anal Biochem 1971;47:389-94.

Balasubramanian KA, Manohar M, Mathan VI. An unidentified inhibitor of lipid peroxidation in intestinal mucosa. Biochem Biophys Acta 1988;962:51-8.

Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S transferase in rat lung and liver. Biochem Biophys Acta 1979;582:67-8.

Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, et al. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 1990;186:464-78.

Krishnamoorthy P, Vaithinathan S, Vimalrani A, Bhuvaneswari A. Effect of Terminalia chebula fruit extract on lipid peroxidation and antioxidative system of testis of albino rats. African J Biotechnol 2007;6:1888-91.

Jadona A, Monika B, Sangeeta S. Protective effect of Terminalia bellerica Roxb. and gallic acid against CCl4 induced damage in rats. J Ethnopharmacol 2006;109:214-8.

Alexandre AA, Graf U, Guperres ZR, Warwick EK, Mario AS. Protective effects of proanthocyanidines of grape (Vitis vinifera L.) seeds on DNA damage induced by doxorubicin in somatic cell of Drosophila melanogaster. Food Chem Toxicol 2009; 31:1-2.

Kaur P, Amandeep W, Kumar S, Kaur S. Antigenotoxic activity of polyphenolic rich extracts from Aegle marmelos (L) correa in human blood lymphocytes and E.coli PQ 37. Nat Prod Rec 2009;3:68-75.

Hanaa H, Baky AE, Farouk KE, Gamal SE. Phenolics from Spirulina maxima: Over production and in vitro protective effect of its phenolics on carban tetrachloride induced hepatotoxicity. J Med Plants Res 2009;3:24-30.

Sing R, Rao HS. Hepatoprotective effect of the pulp/seed of Aegle marmelos correa ex Roxb against CCl4 induced liver damage in rats. Intl J Green Pharmacol 2008;2:232-4.

Senthilkumar GP, Subramanian SP. Biochemical studies on the effect of Terminalia chebula on the levels of glycoproteins in streptozotocin-induced experimental diabetes in rats. J Appl Biomed 2008;6:105-15.

Shamim AQ, Warda A, Sultana V. The effect of Phyllanthus emblica Linn on Type-ll diabetis, triglycerides and liver – specific enzyme. Pakistan J Nutr 2009;8:125-8.


  • There are currently no refbacks.