A study on free radical-induced renal toxicity due to cyclophosphamide and its amelioration with N-acetyl cysteine


Affiliations

  • College of Veterinary Science, Hyderabad, Department of Pharmacology & Toxicology, College of Veterinary Science, Andhra Pradesh, 500030, India
  • College of Veterinary Science, Hyderabad, Department of Pharmacology & Toxicology, Andhra Pradesh, 500030, India

Abstract

A study was conducted to evaluate the protective effect of N-acetyl cysteine (NAC) against experimentally-induced renal toxicity due to cyclophosphamide (CYP) in rats. Three groups (2, 3 and 4) of female Wistar Kyoto rats with 6 animals in each group were administered with CYP @ 40 mg/kg bwt on 1st, 3rd, 5th and 7th day by intraperitoneal route. Group 2 remained as toxic control. Group 3 received NAC from 1st to 14th day @ 0.2% of feed and group 4 was treated with NAC from 8th to 14th day. Group 1 was maintained on basal diet for 14 days. The study revealed a significant (P < 0.05) increase in the concentration of serum creatinine, blood urea nitrogen, thiobarbituric acid reacting substances (TBARS) and protein carbonyls, and a decrease in the reduced glutathione (GSH) concentration of kidney. These results are suggestive of CYP-induced oxidative stress resulting in renal damage. Feeding of NAC could not offer protection against CYPinduced renal damage in this study.

Keywords

Cyclophosphamide (CYP), N-acetyl cysteine (NAC), Oxidative stress

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