In-vitro Antioxidant and Anti-lipid Peroxidation Activity of Ethanolic Extracts of Bougainvillea shubhra, Bougainvillea peruviana and Bougainvillea bhuttiana Golden Glow: A Comparative Study


Affiliations

  • Dr. D.Y Patil University, School of Biotechnology and Bioinformatics, Navi Mumbai, 400 614, India
  • D.Y Patil University, School of Biotechnology and Bioinformatics, Navi Mumbai, Maharashtra, 400 614, India

Abstract

The aim of our present study was to evaluate the antioxidant and anti-lipid peroxidation activity of ethanolic extracts of Bougainvillea species viz B. shubhra, B. peruviana and B. bhuttiana golden glow. Phytochemical constituents viz. total phenolics, flavonoids and tannins were assayed using standard protocol. The antioxidant activity was studied by DPPH assay, FRAP assay, reducing power assay and in-vitro inhibition of lipid peroxidation. Of the three species, B. bhuttiana golden glow showed highest amount of phenols (6.78±0.001), flavonoids (27.7±0.012) and tannin (11.08±0.008) contents. It also showed highest antioxidant activity of 15.15± 0.008 and 8.08± 0.018 as determined by FRAP assay and reducing power assay respectively. The percent DPPH radical scavenging activity was 82.72% and in-vitro inhibition of lipid peroxidation in mitochondrial membrane was also found to be highest in B. bhuttiana golden glow.

Keywords

Antioxidant activity, flavonoids, lipid peroxidation, DPPH

Subject Discipline

Pharmacy and Pharmacology

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References

Khalaf NA, Shakya AK, Al-Othman A, et al. Antioxidant activity of some common plants. Tur J Biology. 2008;32(1):51–5.

Patel VR, Patel PR, Kajal SS. Antioxidant activity of some selected medicinal plants in western region India. Adv Biol Res. 2010; 4:23–6.

Elumalai A, Eswariah CM, Chowdary VCH, et al.Screening of Thrombolytic activity of Bougainvillea glabra leaves extract by Invitro. Asian J Res Pharm Sci. 2012;2(4):134–6.

Umamaheswari A, Shreevidya R, Aparna Nuni. In vitro Antibacterial Activity of Bougainvillea spectabilis Leaves Extracts. Adv in Biol Res. 2008; 2(1-2):1–5.

Joshny J, Devi RD, Hari VBN. Anti-Cancer and Anti-Microbial Activity of Hydro Alcoholic Extract of Bougainvillea glabra. IntJ of Curr Pharma Rev and Res.2013; 3(4):79–85.

Venkatachalam RN, Kanchanlata S, Marar T. Bougainvillea spectabilis, a good source of antioxidant phytochemicals.RJPBCS. 2012; 3(3):605.

Joshi DD, Mujumdar AM, Narayanan CR. Antiinflammatory activity of Bougainvillea spectabilis leaves.Ind J of Pharma Sciences.1984; 46(5):187–8.

Balasaraswathi R, Sadasivam S, Ward M, Walker JM. An antiviral protein from Bougainvillea spectabilis roots; Purification and characterization. Phytochemistry. 1998;47(8):1561–5.

Edwin E, Sheeja E, Toppo E, et al. Effect of antimicrobiano, antiulcerosoy antidiarreico de las hojas de bouganvilla (Bougainvillea glabra choisy) Anti-diarrhoeal, anti ulcer and antimicrobial activities of leaves of Bougainvillea glabra choisy. Ars Pharm. 2007; 48(2):135–44.

Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic phosphotungestic acid reagents.Am J Enol Viticult. 1965; 16:144–58.

Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content inpropolis by two complementary colorimetric methods. J Food Drug Anal. 2002; 10:178–82.

Ordonez AAL, Gomez JD, Vattuone MA, Isla MI. Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chem. 2006; 97:452–8.

McCune LM, Johns T. Antioxidant activity in medicinal plants associated with the symptoms of diabetes mellitus used by the indigenous peoples of the North American boreal forest. J Ethnopharmacol. 2002; 82:197–205.

Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958; 181:1199–200

Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power” the FRAP assay. Anal Biochem. 1996; 239:70–6.

Athukorala Y, Kim KN, Jeon YJ. Antiproliferative andantioxidant properties of an enzymatic hydrolysate frombrown alga Ecklonia cava. Food Chem. Toxicol. 2006;44:1065–74.

Oyaizu M. Studies on products of browning reactions:antioxidative activities of products of browning reaction prepared fromglucosamine. Jap J Nutr. 1986;44:307–15.

Chappell JB, Hansford RG. Preparation of mitochondria from animal tissues and yeasts. In: Birnie GD, editor. Subcellular components: preparation and fractionation.London: Butterworths;1972. p. 77–91.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin phenol reagent. J Biol Chem.1951; 193(1):265–75.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95(2):351–8

Ilavarasan R, Malika M, Venkataraman S. Antiinflammatory and antioxidant activities of Cassia Fistula Linn bark extracts. AJTCAM. 2005; 2(1):70–85

Benavente-Garcia O, Castillo J, Marin FR, Ortuño A, Del-Rio JA. Uses and properties of Citrus flavonoids. J Agric Food Chem. 1997; 45:4505–15.

Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka GI, Nishioka I. Study on the inhibitory effect of tannins and flavonoids against the 1,1-diphenyl-2 picrylhydrazyl radical. Biochem Pharmacol. 1998; 56(2):213–22

Chaires-Martinez L, et al. Determination of Radical Scavenging Activity of Hydroalcoholic and Aqueous Extracts from Bauhinia divaricata and Bougainvillea spectabilis Using the DPPH Assay. PharmacognosyResearch. 2009; 1(5): 238–44.

Yildirm A, Mavi, A, Oktay, M, Kara AA, Algur F,BilalogluV. Comparison of antioxidant and antimicrobial activities of Tilia (Tilia Argentea Desf Ex DC), Sage (Salvia triloba L.), and Black Tea (Camellia sinensis) extracts. Critical Reviews in Food Science and Nutrition. 2000;48(10):5030–4.

Yen GC, ChenHY. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J of Agr and Food Che. 1995; 43(1):27–32.

Devi PK, Suganthy N, Kesika P, Pandian SK. Bioprotective properties of seaweeds: In vitro evaluation of antioxidant activity and antimicrobial activity against food bornebacteria in relation to polyphenolic content. BMC Complementary and Alternative Medicine. 2008; 8:38


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