Identification of bio-active compounds in indigenous Trichoderma asperellem against Fusarium oxysporum f. sp. ciceris: A causal agent of chickpea wilt

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Authors

  • GURURAJ SUNKAD
     Department of Plant Pathology, University of Agricultural Sciences, Raichur – 584104, Karnataka ,IN
  • RANJANA JOSHI
     Department of Plant Pathology, University of Agricultural Sciences, Raichur – 584104, Karnataka ,IN
  • MEGHANA PATIL
     Department of Plant Pathology, University of Agricultural Sciences, Raichur – 584104, Karnataka ,IN

DOI:

https://doi.org/10.18311/jbc/2023/34295

Keywords:

Bio-active compounds, Fusarium oxysporum f. sp. ciceris, GC-MS/MS, Trichoderma asperellum

Abstract

Wilt caused by Fusarium oxysporum f. sp. ciceris (Padwick) is an important soil-borne disease and considered as most serious and widespread disease of chickpea throughout the world. Twenty indigenous isolates of Trichoderma spp. were isolated from the rhizosphere of chickpea in different geographic regions of Karnataka. Among twenty isolates, a higher concentration of volatile compounds was produced by TR-14 (72.22%) followed by TR-19 (66.67%) and TR-9 (65.93%). Further, the efficient strain that is TR-14 was identified as Trichoderma asperellum molecularly and was used to extract metabolites by using solvent extraction technique and subjected to GC-MS/MS analysis. The results have shown presence of twenty-nine compounds at different retention times ranging from 4.910 to 21.868 min. and the mass-to-charge (m/z) ratio from 43 to 190. Out of twenty-nine compounds, the concentration of 2-Imidazol-1-ylmethyl-pyridine 1-oxide (C9H9N3O) was highest with the maximum area (14375440) and retention time (19.842). Apart from this compound, 7-Isopropylidene-5-methyl-2, 3-diazabicyclo (2.2.1) hept-5-ene-2,3 dicarboxylic acid, diethyl ester was present at the highest peak with a retention time of 20.021.

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Published

2023-12-31

How to Cite

SUNKAD, G., JOSHI, R., & PATIL, M. (2023). Identification of bio-active compounds in indigenous <i>Trichoderma asperellem</i> against <i>Fusarium oxysporum</i> f. sp. <i>ciceris:</i> A causal agent of chickpea wilt. Journal of Biological Control, 37(4), 220–225. https://doi.org/10.18311/jbc/2023/34295

Issue

Volume 37, Issue 4, December 2023

Section

Research Articles

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Copyright (c) 2024 GURURAJ SUNKAD, RANJANA JOSHI, MEGHANA PATIL (Author)

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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Received 2023-07-02
Accepted 2023-10-06
Published 2023-12-31

 

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