@article{Siddiqui_Saquib_Ahamed_Ahmad_Al-Khedhairy_Abou-Tarboush_Musarrat_2018, title={Effect of <i>Trans</i>-resveratrol on Rotenone-induced Cytotoxicity in Human Breast Adenocarcinoma Cells}, volume={18}, url={https://www.informaticsjournals.com/index.php/toxi/article/view/21253}, abstractNote={Rotenone, a botanical insecticide is known to cause apoptosis in various cell types. &lt;em&gt;Trans&lt;em&gt;-resveratrol, a natural phytophenol present in red grapes and wine, is also well documented for its antioxidant, anti-inflammatory, anti-mutagenic, and anticarcinogenic activities. Therefore, the present investigations were carried out to assess the protective effect of trans-resveratrol against rotenone-induced cell death in human breast adenocarcinoma (MCF-7) cells. MCF-7 cells were exposed with various concentrations of rotenone for 24 h, and the loss in percent cell viability was evaluated by MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] and neutral red uptake (NRU) assays. A significant decrease in percent cell viability in MCF-7 cells was observed at 50 µM and above concentrations of rotenone, as compared to untreated control. Furthermore, various concentrations (5, 10, and 25 µÎœ) of trans-resveratrol were used to see its protective role on cell viability in rotenone-induced cell death in MCF-7 cells. Pre- or post- treatment of trans-resveratrol for 24 h was given to the cells. The data exhibited a significant dose dependent increase in the percent cell viability under pre- and post-treatment conditions. However, post-treatment of trans-resveratrol for 24 h after rotenone exposure to the cells was relatively less effective. Overall, the results suggest that trans-resveratrol significantly protects MCF-7 cells from rotenone-induced cell death. This model can be used as an effective and economical alternative to animal models for screening the antioxidant activity of a variety of natural compounds/drugs.&lt;/em&gt;&lt;/em&gt;}, number={2}, journal={Toxicology International}, author={Siddiqui, M. A. and Saquib, Q. and Ahamed, M. and Ahmad, J. and Al-Khedhairy, A. A. and Abou-Tarboush, F. M. and Musarrat, J.}, year={2018}, month={May}, pages={105–110} }