Neuroprotective Effect of Naringin on Acrylamideinduced Cytotoxicity in U87MG Cells and Wistar Rats: An <i>in-vitro</i> and <i>in-vivo</i> Study

Sathuluri Vineela; Chinta Manga Devi; Thakur Santhrani

doi:10.18311/ti/2021/v28i1/26267

Authors

Sathuluri Vineela
Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram, Guntur, 522019, Andhra Pradesh
Chinta Manga Devi
Division of Pharmacology, Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Visvavidyalayam, Tirupati, 517502, Andhra Pradesh
Thakur Santhrani
Division of Pharmacology, Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Visvavidyalayam, Tirupati, 517502, Andhra Pradesh

DOI:

https://doi.org/10.18311/ti/2021/v28i1/26267

Keywords:

Acrylamide, Apoptosis, Naringin, Neurotoxicity, Oxidative Stress, U87MG Cells

Abstract

Acrylamide (ACR) is a potent neurotoxic to humans and animals. Neuroprotective effect of naringin was evaluated on ACR induced cytotoxicity using U87MG cells as in-vitro model and rat as in vivo model. ACR (50 mg/kg, i.p.) and Naringin (50 & 100 mg/kg) were administered to rats for 4 weeks. After 4 weeks, rats were sacrificed and sciatic nerves were isolated to determine the biochemical and apoptotic parameters. The exposure of U87MG cells to ACR reduced cell viability. Pretreatment of cells with 5–300 Î¼g/ml naringin before ACR treatment signiï¬cantly attenuated ACR cytotoxicity in a dose-dependent manner. Naringin down-regulated the Bax and up-regulated the Bcl2 protein expression levels and also scavenged ACR induced free radicals in rats. In conclusion, our results indicated that pretreatment with naringin protected cells and rats from ACR-induced cytotoxicity and the neuroprotective effect was due to its antiapoptotic and antioxidant potential.

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