Development and Validation of Simple HPLC Method for Estimation of Flubendiamide in Blood

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Authors

  • Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Science, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut – 250110, Uttar Pradesh ,IN
  • Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, U. P. Pt. Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya, Evam Go Anusandhan Sansthan (DUVASU), Mathura – 281001, Uttar Pradesh ,IN
  • Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, U. P. Pt. Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya, Evam Go Anusandhan Sansthan (DUVASU), Mathura – 281001, Uttar Pradesh ,IN
  • Central Institute for Research on Goat (CIRG), Makhdoom, Farah, Mathura – 281122, Uttar Pradesh ,IN
  • Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, U. P. Pt. Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya, Evam Go Anusandhan Sansthan (DUVASU), Mathura – 281001, Uttar Pradesh ,IN

DOI:

https://doi.org/10.18311/ti/2020/v27i1&2/24930

Keywords:

Flubendiamide, Plasma, HPLC
Toxicology

Abstract

Flubendiamide, novel insecticide used for control of lepidopteron insects on agriculture crops in India. Flubendiamide exposure of to human and animals occurs through ingestion of contaminated feed/fodder, food-products and drinking water etc. Aim of the present study was to develop a simple HPLC method for flubendiamide estimation in blood plasma. Result indicated that HPLC estimation of flubendiamide in blood plasma shows linear standard curve in the range of 0.039-5.0 μg.ml-1 with correlation coefficient (R2) 0.999. Limit of Detection (LOD) and Limit of Quantification (LOQ) was 0.039 μgml-1. Intra-day, inter-day coefficient of variance and mean recovery were found to be 2.70%, 3.50% and approx. 100% respectively, by using C18 column and mixture of acetonitrile: water (70:30, v/v) as mobile phase with flow rate of 0.5 mlmin-1 at 210 λ. Result of this study concluded that this HPLC method is very simple, reliable and repeatable for flubendiamide estimation blood plasma.

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Published

2020-10-01

How to Cite

Mandil, R., Prakash, A., Yadav, R. K. S., Rahal, A., & Garg, S. K. (2020). Development and Validation of Simple HPLC Method for Estimation of Flubendiamide in Blood. Toxicology International, 27(1&2), 23–27. https://doi.org/10.18311/ti/2020/v27i1&2/24930

Issue

Section

Research Articles
Received 2020-02-28
Accepted 2020-06-23
Published 2020-10-01

 

References

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