Development and Validation of Simple HPLC Method for Estimation of Flubendiamide in Blood


  • College of Veterinary and Animal Science, Sardar Vallabhbhai Patel University of Agriculture and Technology, Department of Veterinary Pharmacology and Toxicology, Meerut, Uttar Pradesh, 250110, India
  • College of Veterinary Science and Animal Husbandry, U. P. Pt. Deen Dayal Upadhyay Pashu Chikitsa Vigyan Vishwavidyalaya, Evam Go- Anusandhan Sansthan (DUVASU), Department of Veterinary Pharmacology and Toxicology, Mathura, Uttar Pradesh, 281001, India
  • Central Institute for Research on Goat (CIRG), Mathura, Uttar Pradesh, 281122, India


Flubendiamide, novel insecticide used for control of lepidopteron insects on agriculture crops in India. Flubendiamide exposure of to human and animals occurs through ingestion of contaminated feed/fodder, food-products and drinking water etc. Aim of the present study was to develop a simple HPLC method for flubendiamide estimation in blood plasma. Result indicated that HPLC estimation of flubendiamide in blood plasma shows linear standard curve in the range of 0.039-5.0 μ with correlation coefficient (R2) 0.999. Limit of Detection (LOD) and Limit of Quantification (LOQ) was 0.039 μgml-1. Intra-day, inter-day coefficient of variance and mean recovery were found to be 2.70%, 3.50% and approx. 100% respectively, by using C18 column and mixture of acetonitrile: water (70:30, v/v) as mobile phase with flow rate of 0.5 mlmin-1 at 210 λ. Result of this study concluded that this HPLC method is very simple, reliable and repeatable for flubendiamide estimation blood plasma.


Flubendiamide, Plasma, HPLC

Subject Discipline

Pharmacology & Toxicology

Full Text:


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