An Acute Toxicity Study of <i>Tylophora Indica<i> Leaves Extract in Wistar Rats

Authors

DOI:

https://doi.org/10.18311/ti/2018/v25i3/23751

Keywords:

Acute Toxicity, OECD Guideline 423, Tylophora indica, Wistar Rats

Abstract

The present investigation was carried out to evaluate the safety of hydroalcoholic extract of Tylophora indica leaves after single dose administration to determine its potential toxicity individually as per Organisation for Economic Co-operation and Development [OECD] 423 guideline. General behaviour, adverse effects and mortality parameters were studied for up to 14 days. Extract was found safe at the oral doses of 2000 mg/kg b. wt. There were no apparent toxicity signs and death observed during experimental trial. Gross necropsy examination revealed no abnormal lesions. In view of the dose of Tylophora indica leaves consumed in traditional medicine, it is observed that there is a wide margin of safety for the therapeutic use.

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References

Meyer SA, Kulkarni AP. Hepatotoxicity. Introduction to Biochemical Toxicology. 3rd edn. New York: John Wiley and Sons; 2001. p. 487.

Wolf PL. Biochemical diagnosis of liver diseases. Indian J Clin Biochem. 1999; 14:59–90. PMid: 23105203 PMCid: PMC3453559. https://doi.org/10.1007/BF02869152.

Rao GMM, Rao CV, Pushpangadan P, Shirwaikar A.Hepatoprotective effects of rubiadin, a major constituent of Rubia cordifolia Linn. Journal of Ethnopharmacology. 2006; 103(3):484–90. PMid: 16213120. https://doi.org/10.1016/j.jep.2005.08.073.

Thomas S, Seethe Devi B. Phytochemical and in vitro anthelmintic studies of hydro-alcoholic extract of Costus pictus D. Don. Int J of Pharm Sci. 2013; 5(3):639–41.

Ayurvedic Pharmacopeia of India. Part I, Vol VI. Appendix6; 2008. p. 247.

OECD/OCDE OECD Guideline for testing of chemicals.Acute Oral Toxicity - Acute Toxicity Class Method; 2011.p. 423.

Raina V, Raina S. The responsiveness of leukocyte adenyl cyclase to tylophorine in asthmatic subjects. Biochemical and Biophysical Research Communications. 1980; 94(4):1074– 7. https://doi.org/10.1016/0006-291X(80)90528-8.

Mujeeb M, Aeri V, Bagri P, Khan SA. Hepatoprotective activity of the methanolic extract of Tylophora indica (Burm. f.) Merill leaves IJGP. 2009; 3(2):125–7. https://doi.org/10.4103/0973-8258.54901.

Published

2019-10-22

How to Cite

Bembde, N. S., Meshram, P. V., Gadhave, P. D., Vaidya, V. M., Sole, S. S., & Rohi, R. R. (2019). An Acute Toxicity Study of <i>Tylophora Indica<i> Leaves Extract in Wistar Rats. Toxicology International, 25(3), 163–168. https://doi.org/10.18311/ti/2018/v25i3/23751