Effect of Monosodium Glutamate on Various Lipid Fractions and Certain Antioxidant Enzymes in Arterial Tissue of Chronic Alcoholic Adult Male Mice
Keywords:Alcohol, lipid peroxidation, monosodium glutamate, oxidative stress and atherosclerosis
AbstractOral ingestion of monosodium glutamate (MSG) to chronic alcoholic adult male mice at dose levels of 4 and 8 mg/g body weight for seven consecutive days caused a significant increase in lipid fractions, lipid peroxidation, xanthine oxidase, whereas the levels of superoxide dismutase, catalase, glutathione, and its metabolizing enzymes like glutathione peroxidase and glutathione reductase were significantly decreased in the arterial tissue. These observations suggested that ingestion of MSG to chronic alcoholic animals had no beneficial effect and thereby, could act as an additional factor for the initiation of atherosclerosis.
World Health Organization (WHO). Global Status Report on alcohol. 2004.
Malik VBT, Ahluwalia P. Studies on effect of MSG on various fractions of lipids and certain carbohydrate metabolic enzymes in liver and blood of adult male mice. ToxicolLett 1994;74:69-77.
Ahluwalia P, Tewari K, Choudhary P. Studies on the effects of monosodium glutamate (MSG) on oxidative stress in erythrocytes of adult male mice. ToxicolLett 1996;84:161-5.
Choudhary P, Malik VBT, Puri S, Ahluwalia P. Studies on the effects of monosodium glutamate on hepatic microsomal lipid peroxidation, calcium, ascorbic acid and glutathione and its dependent enzymes in adult male mice. ToxicolLett 1996; 89:71-6.
Singh K, Ahluwalia P. Studies on the effect of MSG administration on some antioxidant enzymes in the arterial tissue of adult male mice. J NutrSciVitaminol (Tokyo) 2003;49:145-8.
Singh K, Ahluwalia P. Alteration in some antioxidant enzymes in cardiac tissue upon MSG administration to adult male mice.Indian J Clin Biochem 2005;20:43-6.
Sharma J, Kuldip S, Ahluwalia P, Arvindpreet K. Alteration in serum homocysteine levels upon ingestion of monosodium glutamate to adult rats. J Life Sci 2009;1:103-5.
Ahluwalia P, Jyoti S, Uma S, Kuldip S, Akhtar M. Effect of administration of monosodium glutamate on carbohydrate metabolizing enzymes in intestinal tissue of adult male mice.Inter Toxicol 2005;12:93-6.
Pavlovic V, Pavlovic D, Kocic G, Sokolovic D, Jevtovic ST, CekicS, et al. Effect of monosodium glutamate on oxidative stress and apoptosis in rat thymus. Mol Cell Biochem 2007; 303:161-6.
Wu D, Cederbaum AI. Alcohol, oxidative stress and free radical damage. Alcohol Res Health 2003;27:277-84.
Husain K, Vazquez-Ortiz M, Lalla J. Down regulation of aortic nitric oxide and antioxidant systems in chronic alcohol-induced hypertension in rats. Hum ExpToxicol 2007;26:427-34.
Lieber CS. Alcoholic fatty liver: its pathogenesis and mechanism of progression to inflammation and fibrosis.Alcohol 2004;341:9-19.
Oh SI, Kim CI, Chun HJ, Park SC. Chronic ethanol consumption affects glutathione status in rat liver. J Nutr 1998;128:758-63.
Frings CS, Fendley TW, Dunn RT, Queen CA. Improved determination of serum lipids by the sulfo-phospho-vanillin reaction. ClinChem 1972;18:673-4.
Fiske CH, Subarow Y. The colorimetric determination of phosphorus. J BiolChem 1925;66:375-400.
McGowan MW, Artiss JD, Strandbergh DR, Zak B. A peroxidasecoupled method for the colorimetric determination of serum triglycerides. ClinChem1983;29:538-42.
Zlatkis A, Zak B, Boyle AJ. A new method for the direct determination of serum cholesterol. J Lab Clin Med 1953;41:486-92.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folinphenol reagent. J BiolChem1951;193:265-75.
Beuge JA, Aust SD. Microsomal lipid peroxidation in methods in enzymology.Vol. 52. NY and London: Academic Press;1978.p. 302-10.
Fried R, Fried, LW.Xanthine oxidase (Xanthine dehydrogenase) Method of enzymatic analysis.In: Bergmeyers HU, editor. Vol.2.New York, London:Academic Press. New York, London, 1974. p. 644-9.
Kono Y. Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase.ArchBiochemBiophys1978;186:189-95.
Luck H. Catalase, method of enzymatic analysis. In:Bermeyer HO, editor. New York, London:Academic Press; 1971. p.855-93.
Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61;882-8.
Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: biochemical role as a component of glutathione peroxidase. Science1973;179:588-90.
Williams CHJ, Arscott ID. Glutathione reductase. Methods in enzymology. Vol.17B. in: Tabor H, Tabor DW(eds) Academic Press, New York (USA) 1971.p. 503-9.
Zhang X, Li SY, Brown RA. Ren J. Ethanol and acetaldehyde in alcoholic cardiomyopathy: from bad to ugly en route to oxidative stress. Alcohol 2004;32:175-86.
Vasdev S, Gill V, Singal PK. Beneficial effect of low ethanol intake on the cardiovascular system: possible biochemical mechanisms.Vasc Health Risk Manag 2006;2:263-76.
Albano E. Alcohol, oxidative stress and free radical damage.ProcNutrSoc 2006;65:278-90.
Ceresosimo E, Williams P, Hoxworth B, Lacy W, Abumrad N.Glutamine blocks lipolysis and ketogenesis of fasting. Am J Physiol 1986;250:E248-52.
Xu P, Huecksteadt TP, Harrison R, HoidalJR. Molecular cloning, tissue expression of human xanthine dehydrogenase. Biochem Biophys Res Commun 1994;199:998-1004.
Gill P, Fernando F, Angela C. Encarnation and malondialdehyde: A possible marker of aging. Gerontol 2002;48:209-14.
Marklund SL. Properties of extracellular superoxide dismutase from human lung. Biochem J 1984;220:269-72.
Bast A, Haenen GR. Cytochrome P450 and glutathione: what is the Significance of their interrelationship in lipid peroxidation?Trends. BioSci 1984;9:510-3.
Kim BJ, Hood BL, Aragon RA, Hardwick JP, Conrads TP, Veenstra TD, et al. Increased oxidation and degradation of cytosolic proteins in alcohol-exposed mouse liver and hepatoma cells. Proteomics 2006;6:1250-60.
Onyema OO, Farombi EO, Emerole GO, Ukoha AI, Onyeze GO. rats.Indian J Biochem Biophys 2006;43:20-4.
Padmini E, Sundari BT. Erythrocyte glutathione depletion impairs resistance to haemolysis in women consuming alcohol. J ClinBiochemNutr 2008;42:14-20.
Nakazawa H, Genka C, Fujishima M. Pathological aspects of active oxygens/ free radicals.JpnJ Physiol 1996;46:15-32.
Blum J, Fridovich I. Inactivation of glutathione peroxidase by superoxide radical. Arch BiochemBiophys 1985;240:500-8.
Raes M, Michiels C, Remacle J. Comparative study of the enzymatic defense systems against oxygen-derived free radicals: the key role of glutathione peroxidase. Free RadicBiol Med 1987;3:3-7.
Lapennna D, de Gioia S, Ciofani G, MezzettiA, Ucchino S, Calafiore AM, et al. Glutathione related antioxidants defence in human atherosclerotic plaques. Circulation 1998;97:1930-4.
Gul M, Kutay FZ, Temocin S, Hanninen O. Cellular and clinical implications of glutathione. Indian J ExpBiol 2000;38:625-34.