Induction of Chronic Renal Failure in Goats Using Cisplatin: A New Animal Model

Authors

Keywords:

Blood urea nitrogen, chronic renal failure, cisplatin, GGT, glomerular filtration rate, goat, plasm creatinine

Abstract

Cisplatin was administered at the dose rate of 30 mg m"‘2 daily intravenously consecutive for 7 days in goats. Blood samples (2 ml) were collected from each goat at ‘0' hr and then at weekly interval and centrifuged immediately at 3000 rpm for 20 min to separate plasma, which were used for estimation of blood urea nitrogen (BUN), plasma creatinine (CRT), gamma glutamyltransferase (γGT), and glomerular filtration rate (GFR). Total volume of urine of each goat was recorded, and 5 ml of urine samples were collected for estimation of GFR. Blood urea nitrogen started to increase significantly from 7 days post"‘dosing and achieved a peak on day 14. Higher values persisted up to 91 days. Plasma creatinine level was significantly higher in all samples on day 7 onwards, and it was maintained up to day 91 post"‘dosing compared to control samples (‘0' day) whilst GFR declined significantly from day 7 and attained a minimum values on day 70. GFR was almost <60% up to 91 days. The signs like emaciation, loss of body weight, and oliguria were observed. The values of all 4 biomarkers showed a chronic renal failure in goats.

Downloads

Download data is not yet available.

References

Yao X, Panichpisal K, Kurtzman N, Nugent K. Cisplatin nephrotoxicity: A review. Am J Med Sci 2007;334:115"‘24.

The Merck Veterinary Manual, 50th Anniversary ed. N.J., U.S.A.: Merck and Co., Inc White House Station; 2008.

Kim HJ, Han KS, Chung YK, Chang MS, Lee MG. Pharmacokinetic changes of a new proton pump inhibitor, YJA"‘20379"‘8, after intravenous and oral administration to rats with uranyl nitrate"‘induced acute renal failure. Res Commun Mol Pathol Pharmacol 1998;102:43"‘56.

Dutta B, Mandal TK, Chakraborty AK. Pharmacokinetics of cefotaxime in goats with experimentally induced kidney damage.Indian J Pharmacol 2003;35:173"‘6.

Shaktidevan RK, Jha KC, Sar TK, Das SK, Chatterjee US, Chakraborty AK, et al. Effect of induced surgical stress and acute renal failure on disposition kinetics of ceftriaxone in goats.Indian J Pharmacol 2005;37:186"‘8.

Daugaard G. Cisplatin nephrotoxicity: Experimental and clinical studies. Danish Med Bull 1990;37:1"‘12.

Lee AS, Kang DG, Yaun JM, Woo WH, Kim YC, Sohn EJ, et al. Butein ameliorates renal concentrating ability in disposition induced acute renal failure in rats. Biol Pharmacol Bull 2004;27:366"‘70.

Zhou H, Kato A, Miyaji T, Yasuda H, Fujigaki Y, Yamamoto T, et al. Urinary marker for oxidative stress in kidney un cisplatin"‘induced acute renal failure in rats. Nephrol Dial Transplant 2006;21:616"‘23.

Kaneko JJ, Harvey JW, Bruss ML. Clinical Biochemistry of Domestic Animals. 5th ed. USA: Academic Press; 1997. p. 468"‘73.

Coulambe SS, Fawreon S. New the semimicro method determination of urea. Clin Chem 1963;9:23"‘82.

Varly H. Practical Clinical Biochemistry. New Delhi, India: Arnold"‘Heinemann Publishers (India) Pvt. Ltd.; 1975.

Tate SS, Meister A. Gamma"‘Glutamyl transpeptidase from kidney.Methods Enzymol 1985;113:400"‘19.

Cockkroft DW, Gault MH. Prediction of creatinine clearance from serum creatinine. Nephrons 1976;16:34"‘41.

Snedecor GW, Cochran WG. Statistical Methods. Ames, IA: Iowa State University Press; 1976.

Asna N, Lewy H, Ashkenazi IE, Deutsch V, Peretz H, Inbar M, et al. Time dependent protection of amifostine from renal and hematopoietic cisplatin induced toxicity. Life Sci 2005;4;76:1825"‘34.

Wein. Campbell"‘Walsh Urology, 9th ed. Philadelphia: Saunders, An Imprint of Elsevier; 2007. p. 741"‘7.

Wainford RD, Weaver RJ, Stewart KN, Brown P, Hawksworth GM.Cisplatin nephrotoxicity is mediated by gamma Glutamyl transpeptidase, not via a C"‘s Lyase governed biotransformation pathways. Toxicology 2008;249:184"‘93.

Arany I, Safirstein RL. Cisplatin nephrotoxicity. Semin Nephrol 2003;23:460"‘4.

Published

2018-08-09

How to Cite

Mishra, A., Chatterjee, U. S., & Mandal, T. K. (2018). Induction of Chronic Renal Failure in Goats Using Cisplatin: A New Animal Model. Toxicology International, 20(1), 56–60. Retrieved from https://www.informaticsjournals.com/index.php/toxi/article/view/21703

Issue

Section

Original Research

Most read articles by the same author(s)