Trans‑Resveratrol Restores the Damages Induced by Organophosphate Pesticide‑Monocrotophos in Neuronal Cells


Affiliations

  • Integral University, Department of Biotechnology

Abstract

The restorative potential of trans‑resveratrol (RV) was investigated in a rat neuronal cell line (PC12) exposed to organophosphate pesticide‑monocrotophos (MCP). RV shows significant protection against MCP‑induced alterations in PC12 cells by restoration of oxidative stress‑mediated apoptosis and cytotoxicity. RV treatment significantly reduced reactive oxygen species (ROS) production and lipid peroxidation, and also restored glutathione levels and mitochondrial membrane potential, in cells receiving MCP. Restoration of markers such as cytochrome c, Bax, Bcl‑2 and caspase‑3 also confirms the effectiveness of RV against MCP‑induced, mitochondria‑mediated apoptosis in PC12 cells. The data identify the protective/restorative potential of RV against MCP‑induced neuronal damages by affecting ROS production and the level of antioxidant defence enzymes.

Keywords

Monocrotophos, oxidative stress, PC12 cells, trans‑resveratrol

Full Text:

References

Ray DE, Richards PG. The potential for toxic effects of chronic, low‑dose exposure to organophosphates. Toxicol Lett 2001;343‑51.

Li Q, Kobayashi M, Kawada T. Chlorpyrifos induces apoptosis in human T cells. Toxicology 2009;255:53‑7.

Costa LG. Current issues in organophosphate toxicology. Clin Chim Acta 2006;366:1‑13.

Kashyap MP, Singh AK, Siddiqui MA, Kumar V, Tripathi VK, Khanna VK, et al. Caspase cascade regulated mitochondria mediated apoptosis in monocrotophos exposed PC12 cells. Chem Res Toxicol 2010;23:1663‑72.

Kashyap MP, Singh AK, Kumar V, Tripathi VK, Srivastava RK, Agrawal M, et al. Monocrotophos induced apoptosis in PC12 cells: Role of xenobiotic metabolizing cytochrome P450s.PLoS One 2011;6:e17757.

Kashyap MP, Singh AK, Kumar V, Yadav DK, Khan F, Jahan S, et al. Pkb/Akt1 mediates Wnt/GSK3b/b‑catenin signaling induced apoptosis in human cord blood stem cells exposed to organophosphate pesticide‑monocrotophos. Stem Cells Dev 2013;22:224‑38.

Singh AK, Kashyap MP, Jahan S, Kumar V, Tripathi VK, Siddiqui MA, et al. Expression and inducibility of cytochrome P450s (CYP1A1, 2B6, 2E1, 3A4) in human cord blood CD34(+) stem cell‑derived differentiating neuronal cells. Toxicol Sci 2012;129:392‑410.

Kazi AI, Oommen A. Monocrotophos induced oxidative damage associates with severe acetylcholinesterase inhibition in rat brain. Neurotoxicology 2012;33:156‑61.

Li F, Gong Q, Dong H, Shi J. Resveratrol, a neuroprotective supplement for Alzheimer’s disease. Curr Pharm Des 2012;18:27‑33.

Huang TC, Lu KT, Wo YY, Wu YJ, Yang YL. Resveratrol protects rats from Ab‑induced neurotoxicity by the reduction of iNOS expression and lipid peroxidation. PLoS One 2011;6:29102.

Siddiqui MA, Kashyap MP, Kumar V, Al‑Khedhairy AA, Musarrat J, Pant AB. Prophylactic potential of trans‑resveratrol against 4‑hydroxynonenal induced damages in PC12 cells. Toxicol In Vitro 2010;24:1592‑8.

Agarwal M, Kumar V, Kashyap MP, Randhawa GS, Pant AB.Ischemic insult induced apoptotic changes in PC12 cells: Protection by trans‑resveratrol. Eur J Pharmacol 2011;666:5‑11.

Agrawal M, Kumar V, Singh AK, Kashyap MP, Yadav S, Khanna VK, et al. trans Resveratrol protects ischemic PC12 cells by inhibiting the expression of hypoxia associated transcription factors and increasing the levels of antioxidant defense enzymes. ACS Chem Neurosci 2013;4:285‑94.

Srivastava RK, Pant AB, Kashyap MP, Kumar V, Lohani M, Jonas L, et al. Multi‑walled carbon nanotubes induce oxidative stress and apoptosis in human lung cancer cell line – A549. Nanotoxicology 2011;5:195‑207.

Auten RL, Davis JM. Oxygen toxicity and reactive oxygen species: The devil is in the details. Pediatr Res 2009;66:121‑7.

Sgambato A, Ardito R, Faraglia B, Boninsegna A, Wolf FI, Cittadini A. Resveratrol, a natural phenolic compound, inhibits cell proliferation and prevents oxidative DNA damage. Mutat Res 2001;496:171‑80.

Virgili M, Contestabile A. Partial neuroprotection of in vivo excitotoxic brain damage by chronic administration of the red wine antioxidant agent, trans‑resveratrol in rats. Neurosci Lett 2000;281:123‑6.

Nicolini G, Rigolio R, Miloso M, Bertelli AA, Tredici G.Anti‑apoptotic effect of trans‑resveratrol on paclitaxel‑induced apoptosis in the human neuroblastoma SH‑SY5Y cell line.Neurosci Lett 2001;302:41‑4.

Moser VC. Age‑related differences in acute neurotoxicity produced by mevinphos, monocrotophos, dicrotophos, and phosphamidon. Neurotoxicol Teratol 2011;33:451‑7.


Refbacks

  • There are currently no refbacks.