Protective Effect of <i>Curculigo orchioides</i> Extract on Cyclophosphamide"‘Induced Neurotoxicity in Murine Model


  • Drug Discovery Laboratory, Shri Ram Institute of Technology-Pharmacy, Jabalpur
  • Drug Discovery Laboratory, Shri Ram Institute of Technology-Pharmacy, Jabalpur
  • Pharmaceutics Research Laboratory, Shri Ram Institute of Technology-Pharmacy, Jabalpur
  • Pinnacle Biomedical Research Institute, Bhopal, MP
  • Drug Discovery Laboratory, Shri Ram Institute of Technology-Pharmacy, Jabalpur


Antioxidant enzymes, brain, <i>Curculigo orchioides</i>, neuroprotective


Free radicals are one of the frequent products of normal cellular metabolism. Disparity of metabolism and excessive generation of free radicals predisposes to disorders like Parkinson's disease, Alzheimer's disease, and aging phenomenon. Curculigo orchioides Gaertn. is known for "adaptogen” and "aphrodisiac” activity and has been proved for antiasthmatic, estrogenic, antiosteoporotic activity along with protection from cisplatin"‘induced cell damage. C. orchioides was powdered and subjected to soxhlet extraction using methanol. Phytochemical studies and estimation of polyphenols and flavonoids was performed. Acute toxicity studies were performed by Organization for Economic Cooperation and Development OECD guidelines. Animals were treated with cyclophosphamide to induce neurotoxicity. Curculigo orchioides was powdered and subjected to soxhlet extraction using methanol. Catalase, superoxide dismutase, glutathione peroxidase, and lipid peroxidation were estimated by reported methods. C. orchioides (400 mg/kg) significantly promoted restoration of catalase (P < 0.005), superoxide dismutase (P < 0.005), and glutathion (P < 0.05) levels. Similarly, a very significant decrease (P < 0.005) in the levels of malondialdehyde was observed. In all cases as mentioned previously, C. orchioides at dose 200 mg/kg promoted significant (P < 0.05) restoration of enzyme levels. C. orchioides (Kali Musli) is rich source of phytochemicals like flavonoids and polyphenols. Flavonoids and polyphenols are reputed to demonstrate neuroprotective effect. These phytochemicals in the present study might be responsible to demonstrate neuroprotective effect.


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Uttara B, Singh AV, Zamboni P, Mahajan RT. Oxidative stress and neurodegenerative diseases: A review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol 2009;7:65"‘74.

Senthilkumar S, Yogeeta SK, Subashini R, Devaki T. Attenuation of cyclophosphamide induced toxicity by squalene in experimental rats. Chem Biol Interact 2006;160:252"‘60.

Oboh G, Rocha JB. Polyphenols in red pepper (Capsicum annuum var. aviculare) and their protective effect on some pro"‘oxidants induced lipid peroxidation in brain and liver. Eur Food Res Technol 2007;225:239"‘47.

Chauhan NS, Sharma V, Thakur M, Dixit VK. Curculigo orchioides: The black gold with numerous health benefits. Zhong Xi Yi Jie He Xue Bao 2010;8:613"‘23.

Pandit P, Singh A, Bafna AR, Kadam PV, Patil MJ. Evaluation of Antiasthmatic Activity of Curculigo orchioides Gaertn. Rhizomes. Indian J Pharm Sci 2008;70:440"‘4.

Vijayanarayana K, Rodrigues RS, Chandrashekhar KS, Subrahmanyam EV. Evaluation of estrogenic activity of alcoholic extract of rhizomes of Curculigo orchioides. J Ethnopharmacol 2007;114:241"‘5.

Jiao L, Cao DP, Qin LP, Han T, Zhang QY, Zhu Z, et al. Antiosteoporotic activity of phenolic compounds from Curculigo orchioides. Phytomedicine 2009;16:874"‘81.

Kang TH, Hong BN, Jung SY, Lee JH, So HS, Park R, et al. Curculigo orchioides protects cisplatin"‘induced cell damage. Am J Chin Med 2013;41:425"‘41.

Harborne JB. Phytochemical method: A guide to modern techniques of plants analysis. New York: Chapman and Hall; 1983, 11"‘23.

Mustafa RA, Abdul Hamid A, Mohamed S, Bakar FA. Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants. J Food Sci 2010;75:C28"‘35.

Organization for Economic Cooperation and development (OECD). Guideline 423 for testing chemicals. Paris: OECD Guidelines; 2001. p. 1"‘14.

Nitharwal RK, Patel H, Karchuli MS, Ugale RR. Chemoprotective potential of Coccinia indica against cyclophosphamide"‘induced toxicity. Indian J Pharmacol 2013;45:502"‘7.

Gornal AC, Bardawill CJ, David MM. Determination of serum protein by means of biuret reaction. J Biol Chem 1949;177:751"‘66.

Liu J, Simon LM, Phillips JR, Robin ED. Superoxide dismutase (SOD) activity in hypoxic mammalian systems. J Appl Physiol Respir Environ Exerc Physiol 1977;42:107"‘10.

Khynriam D, Prasad SB. Changes in endogenous tissue glutathione level in relation to murine ascites tumor growth and the anticancer activity of cisplatin. Braz J Med Biol Res 2003;36:53"‘63.

Rekka EA, Kourounakis AP, Kourounakis PN. Investigation of the effect of chamazulene on lipid peroxidation and free radical processes. Res Commun Mol Pathol Pharmacol 1996;92:361"‘4.

Oboh G, Akomolafe TL, Adetuyi AO. Inhibition of cyclophosphamide"‘induced oxidative stress in brain by dietary inclusion of red dye extracts from sorghum (Sorghum bicolor) stem. J Med Food 2010;13:1075"‘80.

Hsiao G, Fong TH, Tzu NH, Lin KH, Chou DS, Sheu JR. A potent antioxidant, lycopene, affords neuroprotection against microglia activation and focal cerebral ischemia in rats. In Vivo 2004;18:351"‘6.

Malstrom B, Andreasson L, Reinhammer B. Ed. The Enzymes. X11B, Academic Press, New York; 1975. p. 533.

Cheng L, Kellogg EW 3rd, Packer L. Photoinactivation of catalase. Photochem Photobiol 1981;34:125"‘9.

Coyle JT, Puttfarcken P. Oxidative stress, glutamate, and neurodegenerative disorders. Science 1993;262:689"‘95.

Al"‘Majed AA, Sayed"‘Ahmed MM, Al"‘Omar FA, Al"‘Yahya AA, Aleisa AM, Al"‘Shabanah OA. Carnitine esters prevent oxidative stress damage and energy depletion following transient forebrain ischaemia in the rat hippocampus. Clin Exp Pharmacol Physiol 2006;33:725"‘33.

Essa MM, Subramanian P. Protective role of Pongamia Pinnata leaf extract on tissue antioxidant status and lipid peroxidation in Ammonium chloride induced hyperammonemic rats. Toxicol Mech Methods 2006;16:477"‘83.

Abbasi E, Nassiri"‘Asl M, Shafeei M, Sheikhi M. Neuroprotective effects of vitexin, a flavonoid, on pentylenetetrazole"‘induced seizure in rats. Chem Biol Drug Des 2012;80:274"‘8.

Vauzour D, Vafeiadou K, Rodriguez"‘Mateos A, Rendeiro C, Spencer JP. The neuroprotective potential of flavonoids: A multiplicity of effects. Genes Nutr 2008;3:115"‘26.

Basli A, Soulet S, Chaher N, Mérillon JM, Chibane M, Monti JP, et al. Wine polyphenols: Potential agents in neuroprotection. Oxid Med Cell Longev 2012;2012:805762.

Mo H, Chen Y, Huang L, Zhang H, Li J, Zhou W. Neuroprotective effect of tea polyphenols on oxyhemoglobin induced subarachnoid hemorrhage in mice. Oxid Med Cell Longev 2013;2013:743938.



How to Cite

Ramchandani, D., Ganeshpurkar, A., Bansal, D., Karchuli, M. S., & Dubey, N. (2018). Protective Effect of <i>Curculigo orchioides</i> Extract on Cyclophosphamide"‘Induced Neurotoxicity in Murine Model. Toxicology International, 21(3), 232–235. Retrieved from



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