Effect of Dietary Vanaspati Alone and in Combination with Stressors on Sero-biochemical Profile and Immunity in White Leghorn Layers

Authors

  • Department of Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad - 500 030
  • Department of Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad - 500 030
  • College of Veterinary Science, Korutla - 505 326
  • Department of Pharmacology and Toxicology, College of Veterinary Science, Tirupati - 517 502

Keywords:

Immunity, layers, white leghorn, vanaspati

Abstract

A total of 160 White Leghorns of 20 wk age were divided randomly into eight groups. Groups 1, 3, 4 and 5 were fed basal feed and the rest were fed 5% vanaspati supplemented feed until 42 wk of age. From 42 to 54 wk, groups 3, 4 and 5 were fed 1% ferrous sulfate, 100 ppm chlorpyrifos (CPS) and 100 ppm cadmium, respectively, along with basal feed and groups 6, 7 and 8 were fed similar stressors, respectively, along with 5% vanaspati. Groups 1 and 2 served as controls for basal feed and 5% vanaspati feed. Alkaline phosphatase (ALP), alanine transaminase (ALT), total protein, albumin, globulin, A/G ratio, total cholesterol, high density cholesterol (HDL), triglycerides, creatinine, hemagglutination inhibition (HI) titer, and phytohemagglutination (PHA) index were studied. Supplementation of vanaspati resulted in a significant reduction in PHA, cholesterol, albumin and HI titer. Cadmium significantly increased ALP, AST, creatinine and paradoxically increased HDL cholesterol and HI titers. Vanaspati along with cadmium showed similar effects. Administration of CPS lowered PHA index, whereas supplementation along with vanaspati decreased the HI titers and increased the PHA index. Supplementation of vanaspati alone and in combination revealed harmful effects and aggravated the toxicities of CPS and cadmium. Hence, it is concluded that consumption of vanaspati could be harmful.

Downloads

Download data is not yet available.

References

Willett WC, Ascherio A. Trans fatty acids: Are the effects only marginal? American J Public Hlth 1994;84:722-4.

Singh RB, Niaz AM, Ghosh S. Association of trans fatty acids (vegetable ghee) and clarified butter (Indian ghee) intake with higher risk of coronary heart disease in rural and urban populations with low fat consumption. Intl J Cardiol 1996;56:289-98.

Mukherjee AK. India's health: Today and tomorrow. J Indian Med Assoc 1995;93:312-5.

Reddy KS, Yusuf S. Emerging epidemic of cardiovascular disease in developing countries. Circulation 1998;97:596-601.

Chambers JC, Obeid OA, Refsum H. Plasma homocysteine concentrations and risk of coronary heart disease in UK Indian Asians and European men. Lancet 2000;355:523-7.

Ascherio A. Trans fatty acids and coronary heart disease. New Engl J Med 1999;340:1994-8.

Van Barneveld RJ. Physical and chemical contaminants in grains used in livestock feed. Aust J Agric Res 1999;50:807-23.

Cunningham CH. A laboratory guide in virology. 6th Ed. USA: Burgers Publishing Company; 1966. p. 24-34.

Edelman AS, Sanchez PL, Robinson ME, Hochwald GM, Thorbecke GJ. Primary and secondary wattle swelling response to phytohemagglutinin as a measure of immunocompetence in chickens. Avian Dis 1985;30:105-11.

Kaneko JJ, Harvey JW, Michael LB. Clinical Biochemistry of Domestic Animals. 5th ed. New York: Academic Press; 1997.

Demerdesh FM, Yousef MI, Kedwany FS, Baghdadi HH. Cadmium induced changes in lipid peroxidation, blood haematology, biochemical parameters and semen quality of male rats, Protective role of vitamin E and β-carotene. Food Chem Toxicol 2004;42:1563-71.

Kara H, Karatas F, Canatan H, Serv K. Effects of exogenous metallothionein on acute cadmium toxicity in rats. Biol Trace Ele Res 2005;104:223-32.

Yadav N, Dogna RKS, Khan MY, Khandelwal. Prevention of acute cadmium toxicity by Picroliv. Human Exp Toxicol 2005;26:529-38.

Mackness AB, Mackness MI, Arrol S, Turkie W, Durrington PN. Effect of the molecular polymorphisms of human paraoxonase (PON1) on the rate of hydrolysis of paraoxon. Br J Pharmacol 1996;122:265-8.

Richardson RJ. Assessment of the neurotoxic potential of chlorpyrifos relative to other organophosphorus compounds: A critical review of the literature. J Toxicol Environ Haelth 1995;44:135-65.

Peter JH. Regulation of cholesterol biosynthesis by diet in humans. Am J Clin Nutr 1997;66:438-46.

Dulmelis G. Effect of dietary fatty acids, time of feeding and immune response in poultry. Thesis submitted to Oregon State University in partial fulfillment of requirement for the degree of Master of Science. United States: Oregon State University; 2009.

Robohm RA. Paradoxical effects of cadmium exposure on antibacterial antibody response in two fish species: Inhibition in cummers (Tautogolabrus adspersus) and enhancement in striped bass (Morone saxatilis). Vet Immunol Immunopathol 1986;12:251-62.

Ahmad E, Leila A. Home use of vegetable oils, markers of systemic inflammation, and endothelial dysfunction among women. Am J Clin Nutr 2008;88:913-21.

Koga T, Nonaka M, Gu JY, Sugano M. Linoleic and alpha -linoleic acids differently modify the effects of eladic acid on polyunsaturated fatty acid metabolism amd some immune indicies in rats. British J Nutr 1997;77:645-56.

Published

2018-05-21

How to Cite

Raj, M. A., Reddy, A. G., Reddy, A. R., & Adilaxmamma, K. (2018). Effect of Dietary Vanaspati Alone and in Combination with Stressors on Sero-biochemical Profile and Immunity in White Leghorn Layers. Toxicology International, 18(1), 31–34. Retrieved from https://www.informaticsjournals.com/index.php/toxi/article/view/21287

Issue

Section

Original Research

Most read articles by the same author(s)

1 2 > >>