Protective Assessment of Euphorbia neriifolia and its Isolated Flavonoid Against N"‘nitrosodiethylamine"‘induced Hepatic Carcinogenesis in Male Mice: A Histopathological Analysis



Euphorbia neriifolia, flavonoid, liver, necrosis, N"‘nitrosodiethylamine


Aims: The aim of this study was to examine the impacts of N"‘nitrosodiethylamine (DENA), a potent environment carcinogen on liver tissue of mice which was attenuated by isolated flavonoid and hydro"‘ethanolic extract of Euphorbia neriifolia (HEEN) leaves. Materials and Methods: Carcinogenicity was induced in albino mice by a single oral administration of DENA (50 mg/kg body weight). The HEEN (150 and 400 mg/kg body weight), butylated hydroxyanisole (BHA; 0.5 and 1%) and E. neriifolia flavonoid (ENF; 50 mg/kg body weight) were estimated to examine the possible anti"‘cancer potential. Results: DENA exposed animals showed alterations in normal hepatic histo"‘architecture, which comprised of necrosis (N), dilated sinusoids and vacuolization of the cells. Mice treated with E. neriifolia lower (ENL) and higher (ENH) dose and ENF before intoxicated with DENA showed that the liver cells were normal, with very little necrosis (Day 31). On the other hand, BHA higher (BHAH) and lower (BHAL) dose failed to diminish the abnormalities caused by the DENA. Conclusion: Results of the present study suggests that the ENH and ENF protects the hepatic tissue against DENA"‘induced hepatic carcinoma. The results could also be expressed in the order of ENH> ENF> ENL> BHAH> BHAL.


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Gao J, Xie L, Yang WS, Zhang W, Gao S, Wang J, et al. Risk factors of hepatocellular carcinoma – Current status and perspectives. Asian Pac J Cancer Prev 2012;13:743"‘52.

Maideen NM, Velayutham R, Manavalan G. Activity of Prosopis cineraria against N"‘nitrosodiethylamine induced liver tumors by regulating the levels of tumor marker, lipid peroxidation and antioxidants. Asian J Pharm Life Sci 2012;2:1"‘9.

Bansal AK, Trivedi R, Soni GL, Bhatnagar D. Hepatic and renal oxidative stress in acute toxicity of N"‘nitrosodiethylamine in rats. Indian J Exp Biol 2000;38:916"‘20.

Sharma V, Janmeda P, Singh L. N"‘nitrosodiethylamine and carcinogenicity: An over review. Int J Environ Rehabil Conserv 2012;3:56"‘67.

IARC. Overall Evaluations of Carcinogenicity. IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Humans, Overall evaluation of carcinogenicity: An updating of IARC Monographs. Supplement 7. Lyon, France: Int Agency Research Cancer; 1987. 1"‘42, p. 440.

Pracheta, Sharma V, Paliwal R, Sharma S, Singh L, Janmeda BS, et al. Chemo"‘protective activity of hydro"‘ethanolic extract of Euphorbia neriifolia Linn. leaves against DENA"‘induced liver carcinogenesis in mice. Biol Med 2011;3:36"‘44.

Sharma V, Janmeda P, Paliwal R, Sharma S. Antihepatotoxic activity of Euphorbia neriifolia extract against N-nitrosodiethylamineinduced hepatocarcinogenesis in mice. J Chinese Integrat Med 2012;10:1303-9.

Singh L, Pracheta. Cancer: Plant based chemoprevention and therapy: An overview. J Biochem Cell Arch 2012;12:1"‘20.

Singh L, Pracheta, Sharma V, Paliwal R. Bio"‘prospecting anticarcinogenic plants of Rajasthan. J Plant Dev Sci 2012;4:115"‘23.

Harborne JB. A Guide to Modern Techniques of Plant Analysis. Phytochemical Methods. 3rd ed. New Delhi: Springer (India) Pvt. Ltd.; 1998. p. 124.

Bharali R, Tabassum J, Azad MR. Chemomodulatory effect of Moringa oleifera, Lam, on hepatic carcinogen metabolising enzymes, antioxidant parameters and skin papillomagenesis in mice. Asian Pac J Cancer Prev 2003;4:131"‘9.

Pracheta P, Sharma V, Singh L, Paliwal R, Sharma S, Yadav S, et al. Chemopreventive effect of hydroethanolic extract of Euphorbia neriifolia leaves against DENA"‘induced renal carcinogenesis in mice. Asian Pac J Cancer Prev 2011;12:677"‘83.

Sharma V, Pracheta, Paliwal R, Singh L, Sharma V, Sharma SH. Anticarcinogenic potential of Euphorbia neriifolia leaves against N"‘nitrosodiethylamine"‘induced nephrotoxicity in mice. J Biochem Cell Arch 2011;11:393"‘8.

Sharma V, Janmeda P. Chemopreventive Role of Euphorbia neriifolia (Linn) and its isolated flavonoid against N"‘nitrosodiethylamine"‘induced Renal Histopathological damage in male mice. Toxicol Int 2013;20:101"‘7.

Yuan LP, Chen FH, Ling L, Dou PF, Bo H, Zhong MM, et al. Protective effects of total flavonoids of Bidens pilosa L. (TFB) on animal liver injury and liver fibrosis. J Ethnopharmacol 2008;116:539"‘46.

McManus JF, Mowry RW. Staining methods. In: Histological and Histochemical. New York: Harper and Row; 1965. p. 136.

Soni M, Mohanty PK, Jaliwala YA. Hepatoprotective activity of fruits of Prunus domestica. Int J Pharma Biosci 2011;2:439"‘53.

Janani P, Sivakumari K, Parthasarathy C. Hepatoprotective activity of bacoside A against N"‘nitrosodiethylamine"‘induced liver toxicity in adult rats. Cell Biol Toxicol 2009;25:425"‘34.

Tsutsumi M, Ohashi K, Tsujiuchi T, Kobayashi E, Kobitsu K, Kitada H, et al. Disturbance of the cell cycle with colchicine enhances the growth advantage of diethylnitrosamine"‘initiated hepatocytes in rats. Jpn J Cancer Res 1996;87:5"‘9.

Sundaresan S, Subramanian P. Evaluation of chemopreventive potential of garlic extract on N"‘nitrosodiethylamine"‘induced hepatocarcinoma in rats. Pharm Biol 2002;40:548"‘51.

Ismail MF, Ali DA, Fernando A, Abdraboh ME, Gaur RL, Ibrahim WM, et al. Chemoprevention of rat liver toxicity and carcinogenesis by spirulina. Int J Biol Sci 2009;5:377"‘87.

Bansal AK, Bansal M, Soni G, Bhatnagar D. Protective role of Vitamin E pre"‘treatment on N"‘nitrosodiethylamine induced oxidative stress in rat liver. Chem Biol Interact 2005;156:101"‘11.

Sultana S, Ahmed S, Jahangir T. Emblica officinalis and hepatocarcinogenesis: A chemopreventive study in Wistar rats. J Ethnopharmacol 2008;118:1"‘6.

Zhang CL, Zeng T, Zhao XL, Yu LH, Zhu ZP, Xie KQ. Protective effects of garlic oil on hepatocarcinoma induced by N"‘nitrosodiethylamine in rats. Int J Biol Sci 2012;8:363"‘74.

Sharma V, Pracheta. Microscopic studies and preliminary pharmacognostic evaluation of Euphorbia neriifolia (Linn.) leaves. Indian J Nat Prod Resour, 2013;4(4):348-57.

Pracheta, Sharma V, Paliwal R, Sharma S. In vitro free radical scavenging and antioxidant potential of ethanolic extract of Euphorbia neriifolia Linn. Int J Pharm Pharm Sci 2011;3:238-42.

Pracheta, Sharma V, Paliwal R, Sharma S. Preliminary phytochemical screening and In vitro antioxidant potential of hydro-ethanolic extract of Euphorbia neriifolia Linn. Int J Pharm Tech Res 2011;3:124-32.

Sharma V, Janmeda P, Singh L. A Review on Euphorbia neriifolia (Sehund). Spatulla DD 2011;1:107-11.

Sharma V, Pracheta. Remedial effect of Euphorbia neriifolia leaves and isolated flavonoid against N-Nitrosodiethylamine induced renal-carcinogenesis in mice. Indian J Biochem Bio, 2013;50:521-8.

Krinsky NI. Antioxidant functions of carotenoids. Free Radic Biol Med 1989;7:617-35.



How to Cite

Sharma, V., & Janmeda, P. (2018). Protective Assessment of Euphorbia neriifolia and its Isolated Flavonoid Against N"‘nitrosodiethylamine"‘induced Hepatic Carcinogenesis in Male Mice: A Histopathological Analysis. Toxicology International, 21(1), 37–43. Retrieved from



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