Impact of di-n-butyl phthalate on haematology and biochemistry of albino rat


  • Department of Zoology, DAV (P.G.) College, Dehradun-248001
  • Department of Zoology, DAV (P.G.) College, Dehradun-248001
  • Department of Zoology, DAV (P.G.) College, Dehradun-248001


Cholesterol, DBP, lymphocyte and neutrophil


Di-n-butyl phthalate is a low volatile chemical added to plastic, paint, glue, hair spray, toys and house hold products. Human being and animal are exposed to di-n-butyl phthalate through air, water and food contamination in the environment. For present study, a group of six infection free rats were acclimatized under laboratory condition and divided in to two groups three in each. Group I (control) albino rat fed commercial feed with corn oil. Group II albino rats were given 50mg/kg/day di-n-butyl phthalate with corn oil for 2 week. The study revealed that haemaglobin gm%, RBC and lymphocytes were decreased, 14.6% to 7.8%, 6.17-4.19 millions/cmm and 78 to 44% respectively. However, number of neutrophils were increased .A significant decrease in cholesterol level (121- 51 mg/dl), protein (10 to 4.6 gm/dl) and glucose was observed in di-n-butyl phthalate exposed rats.


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Bell, F.P. (1982). Effect of phthalate esters on lipid metabolism in various tissue, cells and organelles. Environ. hlth. perspect., 45: 41-50.

Brandt, K. (1985). Final report on the safety assessment of di butyl phthalate, dimethyl phthalate and di-ethyl phthalate. J. Toxicol., l4: 267-303.

Blount, B.C., Silva, M.J., Candill, S.P. and Needham, L.L. (2000). Level of seven urinary phthalate metabolites in human reference population. Environ. hlth. perspect., 108-979.

Frode, W., Rune, T. and Odd, G.N. (1982). Phthalate esters I: Effect on cytochrome P-450 mediated metabolism in rat liver, lung, serum enzymatic activities and serum protein levels. Arch .Toxicol., 50: 1-10.

Fukuoka, M., Kobayashi, T. and Hayakawa, T. (1994). Mechanism of testicular atrophy induced by di-n-butyl phthalate. Biol. Bull., 17(12): 1609-12.

Fukuoka, M., Nimmi, S., Zhou, Y., Kobayashi, T., Hayaka, T. (1996). Responsiveness of rat non-parenchymal liver cells and spleen cells to decomposition of erythrocytes exposed to di-n-butyl phthalate and its metabolites. Biol. Pharm. Bull., 19(9):1154-1159.

Mushtaq, M., Shrivastava, S.P. and Seth, P.K. (1980). Effect of di-2-ethyl hexyl phthalate on glycogen metabolism in rat liver. Toxicol., 16(2): 153-161.

Marsman, D.(1995). NTP technical report on the toxicity studies of di-n-butyl phthalate administered in feed to F334/N rats and B6c3F1 mice.Toxic. Rep. Ser., 30: 1-5.

Seth, P.K. (1982). Hepatic effect of phthalate esters: Environ. Hlth. Perspect., 45: 27-34.

Short, R.D., Robinson, E.C., Lington. A.W. and Chin E.C. (1987). Metabolic and peroxisome proliferation studies with di (2-ethyl hexyl phthalate) in rats and monkeys. Toxicol. Indian Health, 3: 185-194.

Thomson, C.J., Ross, S.M. and Gaido, K.W. (2004). Di-nbutyl phthalate impairs cholesterol transport and steroid genesis in fetal rat testis through a rapid and reversible mechanism. Endocrinol., 145 :1227-1237.



How to Cite

Kiran, S., Joshi, M., & ., G. (2018). Impact of di-n-butyl phthalate on haematology and biochemistry of albino rat. Toxicology International, 16(2), 117–119. Retrieved from



Original Research