Oxidative stress due to ochratoxin and T-2 toxin either alone or in combination and evaluation of protective role of Curcuma longa, Zingiber officinale, toxichek and activated charcoal
Keywords:Activated charcoal (AC), ochratoxin (OTA), trichothecene (T-2)
AbstractA study was conducted on poultry to evaluate the oxidative stress due to ochratoxin and T-2 either alone or in combination. Broiler chicks were fed OTA @2ppm,T-2 @ 1ppm, Zingiber officinale (rhizome shade dried and powdered @ 0.5%, Curcuma longa rhizome @ 0.5%, toxichek @ 0.1% and activated charcoal @ 0.4% in feed in combinations of 20 groups. The study revealed a significant (p<0.05) increase in the activities of glutathione peroxidase, glutathione reductase, catalase and TBARS in toxic groups (OTA, T-2 and OTA+T-2). Results of the present study suggest that OTA and T-2 either alone or in combination induce oxidative stress and supplementation of Curcuma longa, Zingiber officinale, toxichek and activated charcoal in order showed amelioration by stabilizing the antioxidant defenses.
Ahmed, RS, Vandana Seth, Pasha, SH and Banerjee, BD (1999): Influence of dietary ginger (Zingiber officinale Rose) on oxidative stress induced by malathion in rats. Foods and Chemical Toxicol., 38: 443-450.
AOAC (1995). Official Method of Analysis. 16th ed, Association of Official Analytical Chemist, Washington D.C. Asai, A and Miyazawa, T (2001). Dietary curcuminoids prevent high fat diet induced lipid accumulation in rat liver and epididymal adipose tissue. J. Nutrition, 131: 2932-2935.
Balogun, E, Hoque, M Gong, P Killeen, E, Green, CJ, Foresti, R, Alam J, and Motterline, R (2003). Curcumin activates the haemoxygenase 1 gene expression via regulation of NRFZ and the antioxidant response. Element Biochem. J., 371:887-895.
Britton, C and Machlly, AC (1956). Assay of catalase and peroxidases. Methods in Enzymol., 136: 765-768.
Burmeister, (1971). T-2 toxin production by Fusarium tricinctum on solid substrate. Applied Microbiol., 21: 739-742.
Cao, ZF, Chen, ZG, Guo, P, Zhang, S, Lian, LX, Luo, L and Hu, WM (1993). Scavenging effects of ginger on superoxide anion and hydroxyl radical. Chung Kao Chung Yao Tsa Chin. China Journal of Chinese Materia Medica, 18: 750-751.
Hoehler D and Donald, RM (1996). Influence of vitamins E and C on the toxic effect of ochratoxin A and T-2 toxin in chicks. J. Poultry Sci., 75:1508-15151.
Edrington, TS, Kubena, LF, Harvey, RB and Rottinghaus, GE (1997) influence of a super activated charcoal on the toxin effects of aflatoxin on T-2 toxin in growing broilers. Poult. Sci., 76:1205-1211.
Hasinoff, BB,Rahimtula,AD and Omar ,RF (1990). NADPHCytochrome P-450 reductase promoted hydroxyl radical production by the iron (111)- ochratoxin A complex. Biochem. Biophysics Acta, 1036:78-81.
Huff, WE, Harvey, RB, Kubena, LF and Rottinghaus, GE (1998). Toxic synergism between aflatoxin and T-2 toxin in broiler chickens. Poultry Sci., 67:1418-1422.
Joe, B, Vijayakumar, M and Lokesh,BR (2004). Biological properties of curcumin. Cellular and Molecular mechanisms of action. Critical Rev. Food Sci. Nutrition, 44: 97-111.
Naidu, KA and Thippeswamy, NB (2002). Inhibition of human low density lipoprotein oxidation by active principles from spices. Mol. Cellular Biochem., 229:19-23.
Omar, RF, Hasinoff, BB, Mejilla , F and Rahimtula, AD (1990). Mechanism of ochratoxin A stimulated lipid peroxidation. Biochem. Pharmacol., 40: 1183-1191.
Paglia, DE and Valentine , WN (1967). Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxides J. Lab. Clin. Med., 70: 158-159.
Raghuramulu, N (1983). Manual of Laboratory Techniques. National Institute of Nutrition (ICMR).
Raju, MVLN and Dewagowda, G (2000). Influence of esterified glucomannan on performance and organ morphology, serum biochemistry and haematology in broilers exposed to individual and combined mycotoxicosis (aflatoxin, ochratoxin and T-2 toxin). British Poult. Sci., 41: 640-650.
Rizzo, AF, Alroshi, F, Ahotupa, M, Sankari, S and Elovaara, E (1994): Protective effect of anti oxidant against free radical-mediated lipid peroxidation induced by DON or T-2 toxin. JVMA, 41: 81-90.
Romer, TR, Boiling, TM and McDonald, JL (1978). TLC determination of T-2 toxin and diacetoxyscirpenol in corn and mixed feeds. J. AOAC., 61: 801-807.
Rukshamani, T, Mohan, H and Kamat, JP (2007). Modulation of oxidative damage by natural products. Food Chem., 100: 81-90.
Satish, J, Manwar, P, Thirumurugan, D, Konwar, C and Karna, DK (2007). Effects of Azardirachta indica leaf powder supplementation on broiler performance. Indian Vet. J., 84:159-162.
Singh, I and Singh, VP (2005). Effect of plant extracts on mycelial growth and aflatoxin production by Aspergillus flavus. Indian J Microbiol., 45: 139-142.
Snedecor, WG and Cochran, GW (1994). Statistical Methods. 8th edn., Iowa State University Press, USA.
Subramaniyam, KA, Manohar, M and Matham, VI (1988). An unidentified inhibitor of lipid peroxidation in intestinal mucosa. Biochem. Biophys. Acta, 962: 51-58.
Venkatesan, N (1998). Curcumin attenuation of acute adriamycin myocardial toxicity in rats. British J Pharmacol., 123:425-427.
Venkatesan, N, Punithavathi, D and Arumugam, V (2000). Curcumin prevents adriamycin nephrotoxicity in rats. British J Pharmacol., 129:231-234.
Youkosekiwa, Kikue Kubots and Akio Kobayashi (2000). Isolation of novel glucosides related to gingerdiol from ginger and their antioxidative activities. J. Agri. Food Chem., 48: 373-377.
Zhouy and Xu, R (1992): Antioxidative effect of Chinese drugs. Chung kuo chung yao Tsa chin. China J. Chinese Material Medica, 17:368-369.