Preparation of Giant Unilamellar Vesicles and Solid Supported Bilayer from Large Unilamellar Vesicles: Model Biological Membranes

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Authors

  • Department of Physics, Jadavpur University, Kolkata- 700032 ,IN
  • Department of Physics, Jadavpur University, Kolkata- 700032 ,IN
  • ,IN
  • ,IN

DOI:

https://doi.org/10.18311/jsst/2016/7753

Keywords:

AFM, Dynamic Light Scattering, Model Membranes, Optical Microscopy, Solid Supported Bilayer, Vesicles

Abstract

Giant Unilamellar Vesicles (GUV) and supported planar membranes are excellent model biological systems for studying the structure and functions of membranes. We have prepared GUV from Large Unilamellar Vesicles (LUV) using electroformation and Supported planar Lipid Bilayer (SLB) by vesicle fusion method. LUV was prepared using an extrusion method and was characterized using Dynamic Light Scattering (DLS) and zeta potential measurements. The techniques for obtaining GUV as well as SLB from LUV have been demonstrated. We have directly observed the formation of GUV under phase contrast microscopy. This study will provide some insights into the physico-chemical properties of both nano and micron size vesicles. We believe that this method could be extremely useful for reconstituting various bio-molecules in GUV. We have presented one example where an antimicrobial peptide NK-2 was reconstituted in GUV prepared from LUV. SLB formation was monitored and characterized using Atomic Force Microscopy (AFM).

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Published

2017-04-05

How to Cite

Basu, A., Maity, P., Karmakar, P., & Karmakar, S. (2017). Preparation of Giant Unilamellar Vesicles and Solid Supported Bilayer from Large Unilamellar Vesicles: Model Biological Membranes. Journal of Surface Science and Technology, 32(3-4), 85–92. https://doi.org/10.18311/jsst/2016/7753
Received 2016-10-05
Accepted 2016-10-07
Published 2017-04-05

 

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