@article{S. Badrunissa_Vinitha Ramanath Pai_2023, title={Structural Elucidation of Anti-Pseudomonas Component from <i>Eucalyptus Tereticornis</i>}, volume={23}, url={https://www.informaticsjournals.com/index.php/jnr/article/view/31925}, DOI={10.18311/jnr/2023/31925}, abstractNote={&lt;p&gt;&lt;em&gt;Eucalyptus&lt;/em&gt; species is a well-known medicinal plant from ancient times with diverse biological functions, antimicrobial activity being one of them. This study attempts to isolate a secondary metabolite with antibacterial properties from ethanol extracts of &lt;em&gt;Eucalyptus tereticornis &lt;/em&gt;leaf by antibacterial - against &lt;em&gt;Pseudomonas aeruginosa&lt;/em&gt; - activity-guided procedure. Ethanol extract of the leaf powder was obtained by soxhlation and subjected to liquid-liquid extraction with organic solvents – ethyl acetate, n-hexane, n-butane, and chloroform. The active n-hexane and chloroform extracts were purified by column chromatography, and the components of the active eluant fraction were separated by Thin-layer chromatography (TLC). The purity of the antibacterial compound was checked by high performance liquid chromatography (HPLC), the molecular weight determined by Liquid chromatography-mass spectroscopy (LC-MS) and the structure elucidated and identified by nuclear magnetic resonance (NMR) and by Fourier transform infrared spectrometer (FTIR). TLC of n-hexane extract of liquid-liquid extraction showed a single spot with antibacterial activity. A single major peak was observed on HPLC, and LC-MS revealed that the compound is a formylated phloroglucinol component (FPC) with a molecular weight of 471.3. The NMR and FTIR analysis identified that the isolated compound is Macrocarpal A. This study reveals that the isolated relatively pure anti-&lt;em&gt;Pseudomonas&lt;/em&gt; compound from the leaf extracts of &lt;em&gt;E. tereticornis&lt;/em&gt; is Macrocarpal A, a flavanoid from FPC.&lt;/p&gt;}, number={1}, journal={Journal of Natural Remedies}, author={S. Badrunissa and Vinitha Ramanath Pai}, year={2023}, month={Mar.}, pages={237–246} }