In Vitro Antioxidant and Anti-Diabetic Evaluation of a Polyherbal Formulation

Jump To References Section

Authors

  • Department of Pharmacognosy, Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai – 600116, Tamil Nadu ,IN
  • Department of Pharmacognosy, Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai – 600116, Tamil Nadu ,IN
  • Department of Pharmacognosy, Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai – 600116, Tamil Nadu ,IN
  • Department of Pharmacognosy, Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai – 600116, Tamil Nadu ,IN
  • Department of Pharmacognosy, Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai – 600116, Tamil Nadu ,IN

DOI:

https://doi.org/10.18311/jnr/2022/27294

Keywords:

Aegle marmelous, Churna, Diabetes Mellitus, ? Amylase
Herbal formulation

Abstract

Diabetes Mellitus (DM) is a major metabolic disorder causing morbidity and mortality worldwide. The prominent adverse effects of allopathic drugs are the principal reason for an escalating number of people seeking alternative and complementary-based therapies that may have less or no adverse effects. The poly-herbal formulation Vilva Kudineer Choornam (VKC) is a combination of Siddha medicinal plants for the treatment of diabetes. The present study aimed to investigate the antioxidant and antidiabetic efficacy of poly-herbal formulation by invitro assays. The Aqueous Extract of Formulation (AEF) and Ethanol Extract of Formulation (EEF) were used for the assay. In the present research work, the antioxidant potential of polyherbal formulation (VKC) was evaluated using DPPH radical scavenging and Nitric oxide radical scavenging assay. The IC50 value of AEF was 333.02 ?g/ml and EEF was 86.37 ?g/ml in DPPH radical scavenging assay whereas, in nitric oxide scavenging assay, the IC50 of AEF and EEF were found to be 8.61 ?g/ml and 447.77 ?g/ml, respectively. The VKC was also screened for in-vitro antidiabetic potential by alpha-amylase inhibition assay and it was shown to have significant percentage inhibition of alpha-amylase. The IC50 of EEF and AEF were found to be 868.84 ?g/ ml and 247.09 ?g/ml, respectively. The findings suggest that the polyherbal formulation (VKC) has significant antioxidant and antidiabetic activity. Further in vitro studies using cell lines and other enzymes may be carried out to fully establish the anti-diabetic potential of the polyherbal formulation.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2022-02-14

How to Cite

Shanthi, S., Faheem, ., Ghousiya, S., Divakar, M., & Harish, R. (2022). <i>In Vitro</i> Antioxidant and Anti-Diabetic Evaluation of a Polyherbal Formulation. Journal of Natural Remedies, 22(1), 91–97. https://doi.org/10.18311/jnr/2022/27294

Issue

Section

Short Communication
Received 2021-03-11
Accepted 2021-09-12
Published 2022-02-14

 

References

Patel DK, Prasad SK, Kumar R, Hemalatha S. An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed. 2012; 2(4): 320-30. https://doi.org/10.1016/S2221-1691(12)60032-X

Bartolomeo Lorenzati, Chiara Zucco, Sara Miglietta, Federico Lamberti, Graziella Bruno. Oral hypoglycemic drugs: Pathophysiological basis of their mechanism of action. Pharmaceuticals (Basel). 2010; 3(9):3005–20. https://doi.org/10.3390/ph3093005. PMid:27713388. PMCid:PMC4034109

Zhaolan Liu, Chaowei Fu, Weibing Wang, Biao Xu. Prevalence of chronic complications of Type 2 diabetes mellitus in outpatients – a cross-sectional hospital based survey in urban China. Health Qual Life Outcomes. 2010; 8:62. https://doi.org/10.1186/1477-7525-8-62. PMid:20579389. PMCid:PMC2906445

Giacco F, Brownlee M. Oxidative stress and diabetic complications. Circ Res. 2010; 107:1058–70. https://doi.org/10.1161/CIRCRESAHA.110.223545. PMid:21030723. PMCid:PMC2996922

Sabiu S, O’Neill FH, Ashafa AOT. Kinetics of β-amylase and β-glucosidase inhibitory potential of Zea mays Linnaeus (Poaceae), Stigma maydis aqueous extract: An in vitro assessment. J Ethnopharmacol. 2016; 183:1–8. https://doi. org/10.1016/j.jep.2016.02.024. PMid:26902829

Kim YJ, Chon S, Oh S, Woo J-t, Kim SW, Rhee SY. Analysis of diabetes quality assessment findings and future directions for the appropriate management of diabetes in Korea. Korean J Intern Med. 2019; 34(1):125–36. https://doi.org/10.3904/kjim.2016.136. PMid:28286936. PMCid:PMC6325451

Farzaei F, Morovati MR, Farjadmand F, Farzaei MH. A mechanistic review on medicinal plants used for diabetes mellitus in traditional Persian medicine. J Evid Based Complementary Altern Med. 2017; 22(4):944–55. https:// doi.org/10.1177/2156587216686461. PMid:29228789. PM Cid:PMC5871259

Reddy VP, Urooj A. Antioxidant properties and stability of Aegle marmelos leaves extracts. J Food Sci Technol. 2013; 50(1):135–40. https://doi.org/10.1007/s13197-010-0221-z. PMid:24425898. PMCid:PMC3550959

Deepak S, Pawar A, Shinde P. Study of antioxidant and antimicrobial activities of Andrographis paniculata. Asian J. Plant Sci. Res. 2014; 4(2):31–41.

Ruan ZP, Zhang LL, Lin YM. Evaluation of the antioxidant activity of Syzygium cumini leaves. Molecules. 2008; 13:2545–56. https://doi.org/10.3390/molecules13102545. PMid:18927517. PMCid:PMC6245362

Park A, Ku T, Yoo I. Antioxidant properties of fermented mango leaf extracts. J Cosmet Sci. 2015; 66(1):1–13.

Rao B, Munjal M, Patnayak A, Loganathan K, Kumar G. Phytochemical Composition, antioxidant, antimicrobial and cytotoxic potential of methanolic extracts of Adhatoda vasica (Acanthaceae). Research J. Pharm. and Tech. 2013; 6(9):997–1002.

Kaskoos RA, Hagop AB, Faraj AB, Ahamad J. Comparative antioxidant activity of Gymnema sylvestre, Enicostemma littorale, Momordica charantia and their composite extract. JPP 2015; 4(1):95–8.

Alzohairy MA. Therapeutics role of Azadirachta indica (Neem) and their active constituents in diseases prevention and treatment. Evid. Based Complementary Altern. Med. 2016; 2016. https://doi.org/10.1155/2016/7382506. PMid:27034694. PMCid:PMC4791507

Anonymous, The siddha formulary of India, part-1, 1st ed, Ministry of Health and Family Welfare, New Delhi; 1992.

Anonymous. Indian Pharmacopoeia. vol. 2, Ministry of Health and Family Welfare, Government of India, The Controller of Publications, New Delhi; 1996.

Petchi RR, Vijaya C, Parasuraman S. Anti-arthritic activity of ethanolic extract of Tridax procumbens (Linn.) in Sprague Dawley rats. Pharmacognosy Res. 2013; 5:113–7 https://doi.org/10.4103/0974-8490.110541. PMid:23798886. PMCid:PMC3685759

Wickramaratne MN, Punchihewa JC, Wickramaratne DBM. In-vitro alpha amylase inhibitory activity of the leaf extracts of Adenanthera pavonina. BMC Complem Altern M. 2016; 16:466. https://doi.org/10.1186/s12906-016-1452-y. PMid:27846876. PMCid:PMC5109804

Revathi D, Rajeswari M. In vitro evaluation of nitric oxide scavenging activity of Guettarda speciosa Linn. Int J Sci Res. 2013; 4(9):962–5.

Dsouza DN, Lakshmidevi N. Models to study in vitro antidiabetic activity of plants: A review. Int J Pharm Bio Sci. 2015; 6(3):(B) 732–41.

WHO Fact Sheet Fact sheet. 2015. N°312 [Internet]. [cited 2016 Jan 24]. Available from: http://www.who.int/mediacentre/ factsheets/fs312.

Mehta JL, Rasouli N, Sinha AK, Molavi B. Oxidative stress in diabetes: A mechanistic overview of its effects on atherogenesis and myocardial dysfunction. Int J Biochem Cell Biol. 2006; 38:794-803. https://doi.org/10.1016/j.biocel.2005.12.008. PMid:16442834

Kumar BD, Mitra A, Manjunatha M. A comparative study of alpha-amylase inhibitory activities of common antidiabetic plants of Kharagpur 1 block. Int J Green Pharm. 2010; 4:115–21. https://doi.org/10.4103/0973-8258.63887

Most read articles by the same author(s)