Assessment of Testosterone Biogenesis Enhancement by a Lupeol Derivative Separated from Seeds of Hygrophila spinosa T. Ander in vitro

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Authors

  • Niraj Vyas
     Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat ,IN ORCID logo http://orcid.org/0000-0001-9187-8510
  • Kanan Gamit
     Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat ,IN
  • Manan Raval
     Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat ,IN
  • Samir Patel
     Department of Pharmaceutical Chemistry and Analysis, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat ,IN

DOI:

https://doi.org/10.18311/jnr/2021/26314

Keywords:

Hygrophila spinosa, Lupeol, Leydig Cells, Steroids, Tri-terpenoids, Testosterone
Pharmacogonsy

Abstract

The present study aimed towards assessing the isolated lupeol derivative in vitro for testosterone biogenesis enhancement by the action on isolated rat leydig cells. Studies were carried out to separate a novel bioactive molecule from an Indian medicinal plant, Hygrophila spinosa, used as an aphrodisiac and spermatogenic for years. Unsaponifiable bioactive fraction, prepared from seeds of the plant, was subjected to isolation of phytoconstituents by column and flash chromatography. The isolated compound was characterized by spectral studies as lupeol methyl ether. Later, it was subjected to in vitro studies using isolated leydig cells from rat for its action. Different amounts of isolated compound were incubated with leydig cells for 3 hours and concentration of testosterone after incubation was considered as a parameter. Dehydroepiandrosterone was used as positive control in the studies. The results indicated that the isolated compound was active and enhanced the testosterone biogenesis, as evident from increased amount of testosterone found in a medium treated with compound. This was the first report included isolation, chemical characterization and biological activity of Lupeol methyl ether from Hygrophila spinosa T. Ander.

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Published

2021-10-01

How to Cite

Vyas, N., Gamit, K., Raval, M., & Patel, S. (2021). Assessment of Testosterone Biogenesis Enhancement by a Lupeol Derivative Separated from Seeds of <I>Hygrophila spinosa</I> T. Ander <I>in vitro</I>. Journal of Natural Remedies, 21(4), 317–322. https://doi.org/10.18311/jnr/2021/26314

Issue

Volume 21, Issue 4, October 2021

Section

Research Articles
Crossref
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Europe PMC
Received 2020-10-27
Accepted 2021-04-25
Published 2021-10-01

 

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