A New Spectrophotometric Method for the Estimation of Total Alkaloids in the Stem Bark and Seed of Holarrhena antidysenterica (Linn.) Wall. and in the Ayurvedic Formulation, Kutajarishta


Affiliations

  • B. V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Ahmedabad, Gujarat, 380054, India
  • B. V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Pharmacognosy and Phytochemistry Department, Ahmedabad, Gujarat, 380054, India

Abstract

Objective: To develop a spectrophotometric method for the estimation of total alkaloids in the stem bark, seed and formulations of Holarrhena antidysenterica. Materials and methods: A spectrophotometric method based on the formation of coloured complex between tropaeolin 'OO' reagent and alkaloids. Results: The developed method shows good accuracy and reproducibility. Conclusion: The method developed is simple, precise and accurate and can be adopted for the routine quality control purposes.

Keywords

Holarrhena antidysenterica, Conessine, Spectrophotometric Method.

Subject Discipline

Pharmacy and Pharmacology

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