Transforming Growth Factor β: Cloning and Expression Profiling in the Brain of Catfish Model

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Authors

  • Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad - 500046, Telangana ,IN
  • Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad - 500046, Telangana ,IN
  • Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad - 500046, Telangana ,IN

DOI:

https://doi.org/10.18311/jer/2022/29665

Keywords:

Brain, Development, Expression, Signalling Molecule, Tgf-?

Abstract

Transforming growth factor-beta (tgf-?) is implicated as a signalling molecule and considered a vital regulator for neuronal survival, development, ovarian growth, and many other biological processes. The present study was aimed to analyze the expression of tgf-? and its significance during the early brain and gonadal development. Initially, cloning of partial cDNA of tgf-? from the female brain was performed followed by expression analysis during various developmental stages using quantitative real-time PCR. Cloned tgf-? is approximately 210 bp, which encodes a putative protein of 69 amino acid residues, and showed high similarity with other counterparts. The tissue-specific expression of tgf-? was ubiquitous but significantly higher in brain as well as gonads of both male and female. Further, in ontogeny analysis, tgf-? mRNA levels were significantly greater in 75-, 100- and 125-days post-hatch during development. The pre-spawning stage showed higher expression of tgf-? when compared to all other reproductive stages. Immunolocalization study revealed the presence of Tgf-? protein in the preoptic area-hypothalamus which was well correlated with the mRNA expression in discrete regions of the catfish brain. Expression of tgf-? seems to have a role in the regulation of gonadal function through the brain-pituitary axis.

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Published

2022-04-30

How to Cite

Mamta, S.-K., Anusha, N., & Senthilkumaran, B. (2022). Transforming Growth Factor β: Cloning and Expression Profiling in the Brain of Catfish Model. Journal of Endocrinology and Reproduction, 26(1), 25–33. https://doi.org/10.18311/jer/2022/29665

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Original Research

 

References

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