Disc Gel Electrophoresis in Evaluating Spiders for their Predatory Role in Sugarcane Ecosystem

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Authors

  • Central Institute of Cotton Research, P. B. No.225, G.P.O., Nagpur 440001 ,IN
  • Sugarcane Breeding Institute, Coimbatore-641 007 ,IN

DOI:

https://doi.org/10.18311/jbc/1995/7602

Keywords:

Gel Electrophoresis, Predatory Role, Protein Bands, Spiders, Sugarcane Pests.

Abstract

Use of disc gel electrophoresis towards evaluating spiders for their predatory role in sugarcane system was tested under laboratory conditions. Specific protein bands for different pest types and different species of spiders could be distinctly found. Spider species having fed upon a particular prey type gave a handing pattern that comprised the protein band of both. Method of sample preparation for the electrophoresis, precautions to be taken and further applicability of the method in quantitative assessment of field predation by spiders are discussed.