Mass Multiplication and formulation of Metarhizium anisopliae (Metsch.) Sorokin

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Authors

  • Department of Plant Pathology, College of Agriculture Nagpur 440001, Maharashtra ,IN
  • Department of Plant Pathology, College of Agriculture Nagpur 440001, Maharashtra ,IN
  • Department of Plant Pathology, College of Agriculture Nagpur 440001, Maharashtra ,IN
  • Department of Plant Pathology, College of Agriculture Nagpur 440001, Maharashtra ,IN

DOI:

https://doi.org/10.18311/jbc/2003/3971

Keywords:

Helicoverpa armigera, Media, Metarhizium anisopliae, Pathogenicity, Temperature.

Abstract

Metarhizium anisopliae is a potent microbial insecticide against Helicoverpa armigera (Hübner), a potential threat to successful cultivation of many economically important crops, Pathogenicity study conducted with 108 spores/ml concentration of fungal suspention revealed larval mortality of 100.00,90.00,76.67, and 56.67 per cent against Ist, IInd, IIIrd and IVth instar larvae of H. armigera, respectively. Five media were compared at four temperature levels. Emersons's Yeast Phosphate Soluble Starch (YPSS) medium recorded highest conidial count of 22.00 x 107 spores/ml at 27° C followed. by 250 C, Sabouraud's Dextrose Broth + Yeast (SDB+Y) and Potato Dextrose Broth (PDB) being on par with YPSS at 270 C. Poor cultural growth was recorded in Barners medium at all temperature levels. Amongst the five media used for the mass multiplication studies, Potato Dextrose Broth was found to be hetter in terms of spore production, viability of conidia and its production cost. Bioassay data analyzed by log dose Probit method revealed LC50 values of 6.0x104, 1.2xlO6, 4.8x106 and 5.0x107 spores/ml for Ist, IInd, IIIrd and IVth instar H. armigera larvae, respectively, with very shallow slopes.

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Published

2003-12-06

How to Cite

Wadyalkar, S. R., Wasule, D. L., Bhoyte, S. J., & Wadaskar, R. M. (2003). Mass Multiplication and formulation of <I>Metarhizium anisopliae</I> (Metsch.) Sorokin. Journal of Biological Control, 17(2), 141–146. https://doi.org/10.18311/jbc/2003/3971