Bee flies (Diptera: Bombyliidae) are beneficial insects as pollinators or natural enemies of some agricultural pests. The present paper documents 58 species from 26 genera and six subfamilies (Usiinae, Phthiriinae, Toxophorinae, Heterotropinae, Bombyliinae and Anthracinae) from different parts of Iran. This work also includes the details on collection localities and zoogeographical distribution of the collected species.