Effect of Different Grains as Solid Substrates on Sporulation, Viability and Pathogenicity of <I>Metarhizium anisopliae</I> (Metschnikoff) Sorokin

A. K. Pandey; K. R. Kanaujia

doi:10.18311/jbc/2008/3772

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Authors

A. K. Pandey
College of Forestry & Hill Agriculture, G B. Pant Univ. of Ag. & Tech. Hill Campus, Ranichauri, Tehri Garhwal - 249199 (Uttarakhand) ,IN
K. R. Kanaujia
Department of Entomology, G. B. Pant University of Agriculture and Technology, Pantnagar, 263145, Uttarakhand ,IN

DOI:

https://doi.org/10.18311/jbc/2008/3772

Keywords:

Metarhizium, Pathogenicity, Solid Substrates Fermentation, Spodoptera Sporulation, Viability.

Abstract

Influence of different cereal grains as solid substrates on sporulation, viability and pathogenicity of Metarhizium anisopliae (Metschnikoff) Sorokin was studied under laboratory condition. Among the six grains (viz. maize, sorghum, fingermillet, sorghum, barley and wheat), sorghum as solid substrate resulted in highest spore (6.36 í— 10⁷ conidia ml^âˆ’1) production with spore viability of 86.6 per cent. The production and viability of conidia increased when the substrates were supplemented with sucrose as compared to conidia produced from substrates alone. Conidia produced from fingermillet showed highest virulence against third instar larvae of Spodoptera litura with LC₅₀, value of 1,72 í— 10⁶ conidia ml^âˆ’1 compared to conidia from other sources. However, conidia produced from SDA medium showed highest spore production, viability and virulence to S. litura larvae compared to conidia produced from solid substrates.