Molecular Characterisation of some Indian Anthocorid Predators

T. L. Dheemanth; K. Srinivasa Murthy; Chandish R. Ballal

doi:10.18311/jbc/2015/3232

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Authors

T. L. Dheemanth
ICAR-National Bureau of Agricultural Insect Resources, P B.No. 2491, H.A. Farm Post, Bellary road, Bengaluru – 560024 ,IN
K. Srinivasa Murthy
ICAR-National Bureau of Agricultural Insect Resources, P B.No. 2491, H.A. Farm Post, Bellary road, Bengaluru – 560024 ,IN
Chandish R. Ballal
ICAR-National Bureau of Agricultural Insect Resources, P B.No. 2491, H.A. Farm Post, Bellary road, Bengaluru – 560024 ,IN

DOI:

https://doi.org/10.18311/jbc/2015/3232

Keywords:

Anthocorids, COI, PCR, Phylogeny, Predators.

Abstract

Anthocorid predators widely used as biocontrol agents were characterised for genetic diversity and phylogeny. Cardiastethus exiguus Poppius, Xylocoris flavipes Reuter and Blaptostethus pallescens Poppius were characterised using Cytochrome oxidase I (COI) marker to elucidate the diversity and phylogeny. The sequencing of the COI region at 650bp was compared with sequence of Orius majusculus (Reuter), available at NCBI by BLASTn. The cluster analysis based on dendogram revealed B. pallescens and O. majusculus to be more closely related, while X. flavipes and C. exiguus were distinct. The usefulness of characterisation and phylogenetic relationship among the anthocorid populations for their effective utilisation in pest management is discussed.