Antibacterial and Antifungal Activity of Streptomyces Sp. VITTDK3 Isolated from Ennore Coast, Tamil Nadu, India


Affiliations

  • VIT University, Division of Biomolecules and Genetics, School of Biosciences and Technology, Vellore, 632014, India

Abstract

Actinobacteria isolated from soil samples collected at the Ennore saltern, Tamil Nadu was screened for antibacterial and antifungal activity against common clinical pathogens. The isolate VITDDK3 exhibited more promising antagonistic activity against Escherichia coli, Pseudomonas aeruginosa, Aspergillus flavus and Aspergillus fumigatus. Presence of LL-Diaminopimelic acid and glycine assigned the isolate VITDDK3 to the genus Streptomyces. Further 16S rRNA partial gene sequence was carried out to confirm the chemotaxonomic results. BLAST analysis showed that the strain VITDDK3 possessed 98% similarity with Streptomyces sp. A254Ydz-ZZ but phylogenetic analysis revealed that its closest neighbour was Streptomyces tricolor HBUM175176. The rRNA secondary structure and the restriction sites of VITDDK3 were predicted using Genebee and NEBCutter online softwares respectively.

Keywords

Streptomyces sp. VITDDK3, Antibacterial Activity, Antifungal Activity, Chemotaxonomy, Phylogenetic Analysis.

Subject Discipline

Pharmacy and Pharmacology

Full Text:

References

Vijayakumar R, Muthukumar C, Thajuddin N, Panneerselvam A, Saravanamuthu, Studies on the diversity of actinomycetes in the Palk Strait region of Bay of Bengal, India, Actinomycetologica, 21, 2007, 59-65.

Imran S, Clarisse BFFY, Khaled AS, Shahida H, Hartmut L, Antifungal and antibacterial activities of indigenous Streptomyces isolates from saline farmlands: prescreening, ribotyping and metabolic diversity, World J Microbiol Biotechnol, 25, 2009, 601–610.

Jensen P, William F, Strategies for the discovery of secondary metabolites from marine bacteria. Ecological perspectives, Annu Rev Microbiol, 48, 1994, 559–584.

Imada C, Enzyme inhibitors and other bioactive compounds from marine actinomycetes, Antonie von Leewenhoek, 87, 2004, 59–63.

Zhang L, An R, Wang J, Sun N, Zhang S, Hu J, Kuai J, Exploring novel bioactive compounds from marine microbes, Curr Opinion Microbiol, 8, 2005, 276–281.

Takahashi Y, Omura S, Isolation of new actinomycete strains for the screening of new bioactive compounds, J Gen Appl Microbiol, 49, 2003, 141–154.

Berdy J, Bioactive microbial metabolites: a personal view, J Antibiot, 58(1), 2005, 1–26.

Sanglier JJ, Wellington EMH, Behal V, Feidler HP, Ghorbel RE, Finance C, Hacene M, Kamoun A, Kelly C, Mercer DK, Prinzis S and Tringo C, Novel bioactive compounds from actinomycetes, Res. Microbiol, 144, 1993, 661-663.

Deepika TL, Kannabiran K, A morphological, biochemical and biological studies of halophilic Streptomyces sp isolated from saltpan environment, American Journal of Infectious Diseases, 5 (3), 2009, 207-213.

Strohl WR, Antimicrobials In Microbial Diversity and Bioprospecting, Edited by Bull AT. ASM Press, 2004, 336-355.

Mann J, Natural products as immunosuppressive agents, Nat. Prod. Rep, 18, 2001, 417-430.

Blunt JW, Prinsep MR, Marine natural products, Nat. Prod. Rep. 23, 2006, 26-78.

Bibb MJ, Regulation of secondary metabolism in Streptomycetes, Curr Opin Microbiol, 8, 2005, 208215.

Khamna S, Yokota A, Peberdy JF, Lumyong S, Antifungal activity of Streptomyces spp. Isolated from rhizosphere of Thai medicinal plants. International Journal of Integrative Biology, 6(3), 2009, 143-147.

Deepika TL, Kannabiran K, A report on antidermatophytic activity of actinomycetes isolated from Ennore coast of Chennai, Tamil Nadu, India. International Journal of Integrative Biology, 6(3), 2009, 132-136.

Williams ST, Goodfellow M, Alderson G, Wellington EMH, Sneath PHA, Sackins MJ, Numerical Classification of Streptomyces and Related Genera, Journal of General Microbiology, 129, 1983, 17431813.

Tolbot GH, Bradley J, Edwards JE, Gilbert D, Scheld M, Bartlett, JG, Bad bugs need drugs: An update on the development pipeline from the antimicrobial availability task force of the infectious diseases society of America, Clin. Infect. Dis, 42, 2006, 657668.

Pulikesi M, Baskaralingam P, Elango D, Rayudu VN, Ramamurthi V, Sivanesan S, Air quality monitoring in Chennai, India, in the summer of 2005, J Hazardous Material, 136, 2007, 589-596.

Collins CH, Lyne PM, Grange JM, In: Microbiological methods. London: Butterworth and Heinemann Publishers, 1995, 129-131.

Stanley TW, Elizabeth MS, John GH, Bergeys’s manual of systematic bacteriology, 2001, ISBN: 9780-387-98771-2.

Lechevalier MP, Lechevalier HA, Chemical composition as a criterion in the classification of aerobic actinomycetes, International Journal of Systematic Bacteriology, 20, 1970, 435-443.

Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA, Practical Streptomyces Genetics, 2000.

Stach JEM, Maldonado LA, Ward AC, Goodfellow M, Bull AT, New primers for the class Actinobacteria: application to marine and terrestrial environments, Environ Microbiol, 5, 2003, 828–841.

Farris MH, Oslon JB, Detection of Actinobacteria cultivated from environmental samples reveals bias in universal primers, Letters in Applied Microbiology, 45, 2007, 376–381.

Saitou N, Nei M, The neighbor-joining method: a new method for reconstructing phylogenetic trees, Mol Biol Evol, 24, 1987, 189-204.

Thangadurai N, Srinivasalu S, Jonathan MP, Rajeshwara RN, Santhosh KR, Pre-Tsunami chemistry of sedimentsalong the inner continental shelf off, Ennore, Chennai, southeast coast of India, Indian Journal of Marine Sciences, 34, 2005, 274-278.

Narayana KPJ, Prabhakar P, Vijayalakshmi M, Venkateswarlu Y and Krishna SJP, Study on Bioactive compounds from Streptomyces sp. ANU 6277, Polish Journal of Microbiology, 57(1), 2008, 35-39.

Kiran RK, Amol K, Hardikar BP, Antimicrobial and cytotoxic activity of Streptomyces sp. from Lonar Lake, African Journal of Biotechnology, 8 (23), 2009, 6645-6648.

Augustine SK, Bhavsar SP, Kapadnis BP, A nonpolyene antifungal antibiotic from Streptomyces albidoflavus PU 23, J. Biosci, 30(2), 2005, 201–211.

Flavio L, Raya K, Luisa S, Larissa BMB, Svetlana P, Identification of Nocardiopsis dassonvillei in a Blood Sample from a Child, American Journal of Infectious Diseases, 1 (1), 2005, 1-4.

Masato T, Yasuhiro G, Toru S, Osami T, Kawamura T, Kamimura T, Toshiaki H, SF2809 Compounds, Novel Chymase Inhibitors from Dactylosporangium sp., Journal of Antibiotics, 57(2), 2004.

Rattanaporn S, Kimiko K, Morakot S, Shinji T, Coastal Soil Actinomycetes: Thermotolerant Strains Producing N-Acylamino Acid Racemase, Science Asia, 30, 2004, 123-126.


Refbacks

  • There are currently no refbacks.